Abstract:
:Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase (pdcA), or phosphoglycerate kinase (pgk1) promoters to drive expression of heterologous proteins. All three plasmids use the pdcA terminator for transcription termination, the pyrG gene for restoration of uracil prototrophy, and an ampicillin resistance gene and origin of replication for maintenance in Escherichia coli. We have expressed green fluorescent protein (GFP) and compared transcription and protein accumulation for each of the expression vectors. Accumulation of GFP transcript and protein was directly correlated with the choice of promoter with pdcA > amyA > pgk1. Transcript level appears to parallel GFP protein accumulation. Plasmid copy number had little impact on transcription or protein accumulation. These vectors should be useful for overexpression of heterologous proteins and potentially, metabolic engineering of Rhizopus strains.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Mertens JA,Skory CD,Ibrahim ASdoi
10.1007/s00203-006-0121-9subject
Has Abstractpub_date
2006-07-01 00:00:00pages
41-50issue
1eissn
0302-8933issn
1432-072Xjournal_volume
186pub_type
杂志文章abstract::Bacillus stearothermophilus strains PV 72 and ATCC 12980 carry a crystalline surface layer (S-layer) with hexagonal (p6) and oblique (p2) symmetry, respectively. Sites of insertions of new subunits into the regular lattice during cell growth have been determined by the indirect fluorescent antibody technique and the p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446749
更新日期:1988-01-01 00:00:00
abstract::To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0582-8
更新日期:2010-07-01 00:00:00
abstract::A Gram-stain-negative, strictly aerobic, beige-pigmented, motile, chemoheterotrophic, rod-shaped or ovoid bacterium, designated strain KMU-37T, was isolated from seawater at Najeong Beach in the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1329-y
更新日期:2017-05-01 00:00:00
abstract::Chloroquine inhibited the outgrowth of Bacillus subtilis spores at a 10-fold lower concentration than that required to prevent vegetative growth. Analysis of macromolecular synthesis in outgrowing spores and vegetative cells in the presence of chloroquine indicated that it acted preferentially on transcription. Differ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409659
更新日期:1989-01-01 00:00:00
abstract::The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050465
更新日期:1997-07-01 00:00:00
abstract::Multi- and pan-antibiotic-resistant bacteria area major health challenge in hospital settings. Furthermore,when susceptible bacteria establish surface-attached biofilm populations, they become recalcitrant to antimicrobial therapy. Therefore, there is a need for novel antimicrobials that are effective against multi-dr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0793-2
更新日期:2012-07-01 00:00:00
abstract::Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00690224
更新日期:1976-11-02 00:00:00
abstract::The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0119-3
更新日期:2006-07-01 00:00:00
abstract::Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050484
更新日期:1997-09-01 00:00:00
abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425807
更新日期:1984-01-01 00:00:00
abstract::Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000160
更新日期:2000-05-01 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425171
更新日期:1989-01-01 00:00:00
abstract::CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429118
更新日期:1978-09-01 00:00:00
abstract::Cryptococcus adeliensis was initially described as a psycrophilic species containing a single strain CBS 8351(T) isolated from decayed algae in Terre Adelie (Antartida). Later, a second strain of this species was isolated from an immunosuppressed patient affected by leukaemia in Germany and recently several strains fr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0134-4
更新日期:2006-09-01 00:00:00
abstract::It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-011-0733-6
更新日期:2012-01-01 00:00:00
abstract::The histidine protein kinase SppK is a peptide pheromone-activated kinase that regulates the production of the bacteriocin sakacin P in Lactobacillus sakei. SppK belongs to subfamily 10 of histidine protein kinases (HPKs), which regulate important processes in Gram-positive bacteria, including virulence, competence an...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0049-5
更新日期:2006-01-01 00:00:00
abstract::Glyoxalase I is a ubiquitous enzyme that detoxifies methylglyoxal, which is derived from glycolysis but inhibits the growth of cells from microorganisms to mammals. Here, the structural gene for glyoxalase I ( glo1(+)) from the fission yeast Schizosaccharomyces pombe was identified. Disruption of glo1(+) enhanced susc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0666-4
更新日期:2004-05-01 00:00:00
abstract::Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF01692864
更新日期:1996-03-01 00:00:00
abstract::Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00410891
更新日期:1984-08-01 00:00:00
abstract::Pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. Extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent bra...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00423069
更新日期:1979-03-12 00:00:00
abstract::A combination of gene loss and acquisition through horizontal gene transfer (HGT) is thought to drive Streptococcus thermophilus adaptation to its niche, i.e. milk. In this study, we describe an in silico analysis combining a stochastic data mining method, analysis of homologous gene distribution and the identificatio...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0671-8
更新日期:2011-04-01 00:00:00
abstract::The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425346
更新日期:1976-04-01 00:00:00
abstract::Chromate resistance in Ralstonia metallidurans CH34 is based on chromate efflux catalyzed by ChrA efflux pumps. The bacterium harbors two chromate resistance determinants, the previously known chr(1) on plasmid pMOL28 (genes chrI, chrB(1), chrA(1), chrC, chrE, chrF(1)) and chr(2) on the chromosome (genes chrB(2), chrA...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0492-5
更新日期:2002-12-01 00:00:00
abstract::The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0609-5
更新日期:2003-12-01 00:00:00
abstract::Organic acid production including lactate and acetate is an economically attractive technology that has gained momentum worldwide over the past years. These series of action need to be performed by an esoteric and complex microbial community, in which different members have distinct roles in the establishment of a col...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01676-2
更新日期:2019-11-01 00:00:00
abstract::Chlorella fusca, strain 211-15, cells degreened in a nitrogen-deficient mineral growth medium in the light for 4-6 weeks were regreened for up to 24 hrs in a nitrogen rich medium that leads to synchronous cell division at 24-26 hrs. Structural changes in the plastid membranes during the regreening period were observed...
journal_title:Archives of microbiology
pub_type: 杂志文章
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abstract::The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406170
更新日期:1980-12-01 00:00:00
abstract::The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050505
更新日期:1997-10-01 00:00:00
abstract::Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were stu...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2021-01-02 00:00:00