Influence of chlorobenzoates on the utilisation of chlorobiphenyls and chlorobenzoate mixtures by chlorobiphenyl/chlorobenzoate-mineralising hybrid bacterial strains.


:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be one of the rate-limiting steps in the overall PCB-degradation process. Three hybrid bacteria constructed to have the ability to completely mineralise 2-, 3-, or 4-monochlorobiphenyl respectively, have been studied to establish the range of mono- and diCBAs that can be utilised. The three strains were able to mineralise one or more of the following CBAs: 2-, 3-, and 4-monochlorobenzoate and 3,5-dichlorobenzoate. No utilisation of 2,3-, 2,5-, 2,6-, or 3,4-diCBA was observed, and only a low concentration (0.11 mM) of 2,4-diCBA was mineralised. When the strain with the widest substrate range (Burkholderia cepacia JHR22) was simultaneously supplied with two CBAs, one that it could utilise plus one that it was unable to utilise, inhibitory effects were observed. The utilisation of 2-CBA (2.5 mM) by this strain was inhibited by 2,3-CBA (200 microM) and 3,4-CBA (50 microM). Although 2,5-cba and 2,6-cba were not utilised as carbon sources by strain jhr22, they did not inhibit 2-cba utilisation at the concentrations studied, whereas 2,4-cba was co-metabolised with 2-cba. The utilisation of 2-, 3-, and 4-chlorobiphenyl by strain JHR22 was also inhibited by the presence of 2,3- or 3,4-diCBA. We conclude that the effect of the formation of toxic intermediates is an important consideration when designing remediation strategies.


Arch Microbiol


Archives of microbiology


Stratford J,Wright MA,Reineke W,Mokross H,Havel J,Knowles CJ,Robinson GK




Has Abstract


1996-03-01 00:00:00












  • The use of stable sulfur isotope labelling to elucidate sulfur metabolism by Clostridium pasteurianum.

    abstract::An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: McCready RG,Laishley EJ,Krouse HR

    更新日期:1976-09-01 00:00:00

  • Amelioration effect of chromium-tolerant bacteria on growth, physiological properties and chromium mobilization in chickpea (Cicer arietinum) under chromium stress.

    abstract::In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Shreya D,Jinal HN,Kartik VP,Amaresan N

    更新日期:2020-05-01 00:00:00

  • Characterization of a glucosylglycerol-phosphate-accumulating, salt-sensitive mutant of the cyanobacterium Synechocystis sp. strain PCC 6803.

    abstract::Salt-sensitive mutants of Synechocystis were obtained by random cartridge mutagenesis, and one mutant (mutant 4) was characterized in detail. The salt tolerance of mutant 4 was reduced to about 20% of that of the wild-type. This was caused by a defect in the biosynthetic pathway of the osmoprotective compound glucosyl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hagemann M,Richter S,Zuther E,Schoor A

    更新日期:1996-08-01 00:00:00

  • Glycollate production and excretion by Alcaligenes eutrophus.

    abstract::Autotrophic cultures of the facultative chemolithotroph Alcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Codd GA,Bowien B,Schlegel HG

    更新日期:1976-11-02 00:00:00

  • Microfilament bundles in antheridial nuclei of Achlya ambisexualis E 87.

    abstract::This is the first report of intranuclear microfilaments within gametangial nuclei of oömycetous fungi. Longitudinal sections of four to six microfilaments were frequently observed in meiotic antheridial nuclei of Achlya ambisexualis. Each microfilament measured approximately 7--10 nm in diameter. Spindle tubules (25 n...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Ellzey J,Huizar E,Yanez D

    更新日期:1976-02-01 00:00:00

  • Lactobacillus plantarum ccl gene is non-essential, arginine-repressed and codes for a conserved protein in Firmicutes.

    abstract::Among proteins specifically found in most gram-positive bacteria of the phylum Firmicutes, conserved proteins of the family pfam06177-DUF988-COG4708 are of unknown function. The citrulline cluster-linked (ccl) gene of Lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Arsène-Ploetze F,Nicoloff H,Bringel F

    更新日期:2005-08-01 00:00:00

  • Modulation of nitrogen metabolism of maize plants inoculated with Azospirillum brasilense and Herbaspirillum seropedicae.

    abstract::Maize is highly responsive to the application of nitrogen to achieve high productivity. Inoculation with diazotrophic bacteria can improve plant growth with low N fertilization. The objective was to evaluate the inoculation of two species of diazotrophs on N metabolism in maize plants, in the presence of two concentra...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: da Fonseca Breda FA,da Silva TFR,Dos Santos SG,Alves GC,Reis VM

    更新日期:2019-05-01 00:00:00

  • Physiological roles of mycothiol in detoxification and tolerance to multiple poisonous chemicals in Corynebacterium glutamicum.

    abstract::Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Liu YB,Long MX,Yin YJ,Si MR,Zhang L,Lu ZQ,Wang Y,Shen XH

    更新日期:2013-06-01 00:00:00

  • The correlation of the gene csoS2 of the carboxysome operon with two polypeptides of the carboxysome in thiobacillus neapolitanus

    abstract::The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Baker SH,Lorbach SC,Rodriguez-Buey M,Williams DS,Aldrich HC,Shively JM

    更新日期:1999-10-01 00:00:00

  • Regulation of flavoprotein synthesis studied in vivo in a riboflavin-requiring mutant of Arthrobacter oxidans.

    abstract::The biosynthesis of two flavoproteins, 6-hydroxy-D-nicotine oxidase with covalently bound FAD and 6-hydroxy-L-nicotine oxidase containing non-covalently bound FAD, was studied in wild-type cells and in a riboflavin-requiring mutant of Arthrobacter oxidans. In the mutant cells, the rate of synthesis and the maximal act...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hamm HH,Decker K

    更新日期:1978-10-04 00:00:00

  • Directed modification of a ruminal cellulase gene (CMC-1) from a metagenomic library isolated from Yunnan gayal (Bos frontalis).

    abstract::Gayal (Bos frontalis) of the Yunnan region is well adapted to harsh environmental conditions. Its diet consists predominantly of bamboo, reeds, and woody plants, suggesting that the rumen of this species contains many fiber-degrading bacteria and cellulases. The aim of this study was to identify and modify specific ce...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Wu D,Wang S,Vinitchaikul P,Zhu Y,Zhou X,Gu Z,Leng J,Gou X,Deng M,Sun L,Mao H,Yang S

    更新日期:2020-07-01 00:00:00

  • The fine structure of Micrococcus radiophilus and Micrococcus radioproteolyticus.

    abstract::The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sleytr UB,Silva MT,Kocur M,Lewis NF

    更新日期:1976-04-01 00:00:00

  • Role of respiration and glutathione in cadmium-induced oxidative stress in Escherichia coli K-12.

    abstract::Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pacheco CC,Passos JF,Castro AR,Moradas-Ferreira P,De Marco P

    更新日期:2008-03-01 00:00:00

  • The regreening of nitrogen-deficient Chlorella fusca II. Structural changes during synchronous regreening.

    abstract::Chlorella fusca, strain 211-15, cells degreened in a nitrogen-deficient mineral growth medium in the light for 4-6 weeks were regreened for up to 24 hrs in a nitrogen rich medium that leads to synchronous cell division at 24-26 hrs. Structural changes in the plastid membranes during the regreening period were observed...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pyliotis NA,Goodchild DJ

    更新日期:1975-05-05 00:00:00

  • Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes.

    abstract::Mycothiol is the predominant thiol in most actinomycetes, including Mycobacterium tuberculosis, and appears to play a role analogous to glutathione, which is not found in these bacteria. The enzymes involved in mycothiol biosynthesis are of interest as potential targets for new drugs directed against tuberculosis. In ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Koledin T,Newton GL,Fahey RC

    更新日期:2002-11-01 00:00:00

  • Metabolic regulation of pyruvate kinase isolated from autotrophically and heterotrophically grown Paracoccus denitrificans.

    abstract::Pyruvate kinase (ATP: pyruvate phosphotransferase (EC was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Slabas AR,Whatley FR

    更新日期:1977-10-24 00:00:00

  • Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds.

    abstract::Essential oils (EOs) are products of plant origin and include mixtures of different chemical compounds. These volatile substances have many interesting properties, including antifungal properties. Fungi may develop under field conditions on crops such as wheat or corn and are able to synthesize mycotoxins, which adver...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Perczak A,Gwiazdowska D,Marchwińska K,Juś K,Gwiazdowski R,Waśkiewicz A

    更新日期:2019-10-01 00:00:00

  • The phenol oxidases of the ascomycete Podospora anserina. XII. Affinity of laccases II and III to substrates with different substitution patterns.

    abstract::For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hoffmann P,Esser K

    更新日期:1977-02-04 00:00:00

  • An overview of in silico protein function prediction.

    abstract::As the protein databases continue to expand at an exponential rate, fed by daily uploads from multiple large scale genomic and metagenomic projects, the problem of assigning a function to each new protein has become the focus of significant research interest in recent times. Herein, we review the most recent advances ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审


    authors: Sleator RD,Walsh P

    更新日期:2010-03-01 00:00:00

  • Characterization and localization of phosphatidylglycerophosphate and phosphatidylserine synthases in Rhodobacter sphaeroides.

    abstract::Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km valu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Radcliffe CW,Steiner FX,Carman GM,Niederman RA

    更新日期:1989-01-01 00:00:00

  • Evidence for an arene oxide-NIH shift pathway in the transformation of naphthalene to 1-naphthol by Bacillus cereus.

    abstract::Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Cerniglia CE,Freeman JP,Evans FE

    更新日期:1984-08-01 00:00:00

  • Genome-wide transcriptional changes during the lag phase of Saccharomyces cerevisiae.

    abstract::The set of physiological and metabolic changes occurring immediately after inoculation and during the lag phase is thought to be of vital importance for optimal offset of fermentation. The transcriptional changes taking place during the lag phase after inoculation of a late-respiratory-phase yeast culture into fresh, ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Brejning J,Jespersen L,Arneborg N

    更新日期:2003-04-01 00:00:00

  • Environmental regulation of alcohol metabolism in thermotolerant methylotrophic Bacillus strains.

    abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Arfman N,de Vries KJ,Moezelaar HR,Attwood MM,Robinson GK,van Geel M,Dijkhuizen L

    更新日期:1992-01-01 00:00:00

  • Identification of TTA codon containing genes in Frankia and exploration of the role of tRNA in regulating these genes.

    abstract::The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sen A,Thakur S,Bothra AK,Sur S,Tisa LS

    更新日期:2012-01-01 00:00:00

  • Biosynthesis and cytoplasmic accumulation of a chlorinated catechol pigment during 3-chlorobenzoate aerobic co-metabolism in Pseudomonas fluorescens.

    abstract::A strain of Pseudomonas fluorescens was capable of co-metabolizing 3-chlorobenzoic acid with the production of a chlorinated catechol black pigment. A peroxidase and another enzymatic activity referred to as a polyphenol oxidase were found to be involved in the oxidation of 4-chlorocatechol to 4-chloro-1,2-benzoquinon...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Fava F,Di Gioia D,Romagnoli C,Marchetti L,Mares D

    更新日期:1993-01-01 00:00:00

  • Comparative genomic study of spo0E family genes and elucidation of the role of Spo0E in Bacillus anthracis.

    abstract::The propensity of bacterium to sporulate or retain the vegetative form depends on the amount of phosphorylated Spo0A (Spo0A(-P)), regulated by Spo0E multigene family of phosphatases (Spo0E, YisI and YnzD). Phylogenetic analysis revealed that Spo0E multigene family of phosphatases (SMFP) descends in two distinct clades...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Dubey GP,Narayan A,Mattoo AR,Singh GP,Kurupati RK,Zaman MS,Aggarwal A,Baweja RB,Basu-Modak S,Singh Y

    更新日期:2009-03-01 00:00:00

  • A mechanistic study on SMOB-ADP1: an NADH:flavin oxidoreductase of the two-component styrene monooxygenase of Acinetobacter baylyi ADP1.

    abstract::Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Gröning JA,Kaschabek SR,Schlömann M,Tischler D

    更新日期:2014-12-01 00:00:00

  • Functional analysis of the Streptomyces lividans type I signal peptidases.

    abstract::Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Geukens N,Parro V,Rivas LA,Mellado RP,Anné J

    更新日期:2001-11-01 00:00:00

  • Oxidation and ring cleavage of dibenzofuran by the filamentous fungus Paecilomyces lilacinus.

    abstract::The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Gesell M,Hammer E,Mikolasch A,Schauer F

    更新日期:2004-09-01 00:00:00

  • The SRD2 gene is involved in Saccharomyces cerevisiae morphogenesis.

    abstract::Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Cañizares JV,Pallotti C,Saínz-Pardo I,Iranzo M,Mormeneo S

    更新日期:2002-04-01 00:00:00