Influence of chlorobenzoates on the utilisation of chlorobiphenyls and chlorobenzoate mixtures by chlorobiphenyl/chlorobenzoate-mineralising hybrid bacterial strains.


:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be one of the rate-limiting steps in the overall PCB-degradation process. Three hybrid bacteria constructed to have the ability to completely mineralise 2-, 3-, or 4-monochlorobiphenyl respectively, have been studied to establish the range of mono- and diCBAs that can be utilised. The three strains were able to mineralise one or more of the following CBAs: 2-, 3-, and 4-monochlorobenzoate and 3,5-dichlorobenzoate. No utilisation of 2,3-, 2,5-, 2,6-, or 3,4-diCBA was observed, and only a low concentration (0.11 mM) of 2,4-diCBA was mineralised. When the strain with the widest substrate range (Burkholderia cepacia JHR22) was simultaneously supplied with two CBAs, one that it could utilise plus one that it was unable to utilise, inhibitory effects were observed. The utilisation of 2-CBA (2.5 mM) by this strain was inhibited by 2,3-CBA (200 microM) and 3,4-CBA (50 microM). Although 2,5-cba and 2,6-cba were not utilised as carbon sources by strain jhr22, they did not inhibit 2-cba utilisation at the concentrations studied, whereas 2,4-cba was co-metabolised with 2-cba. The utilisation of 2-, 3-, and 4-chlorobiphenyl by strain JHR22 was also inhibited by the presence of 2,3- or 3,4-diCBA. We conclude that the effect of the formation of toxic intermediates is an important consideration when designing remediation strategies.


Arch Microbiol


Archives of microbiology


Stratford J,Wright MA,Reineke W,Mokross H,Havel J,Knowles CJ,Robinson GK




Has Abstract


1996-03-01 00:00:00












  • Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family.

    abstract::YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Fujisawa M,Wada Y,Tsuchiya T,Ito M

    更新日期:2009-08-01 00:00:00

  • Isolation, characterization, and fermentative pattern of a novel thermotolerant Prototheca zopfii var. hydrocarbonea strain producing ethanol and CO2 from glucose at 40 degrees C.

    abstract::A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Ueno R,Urano N,Suzuki M,Kimura S

    更新日期:2002-03-01 00:00:00

  • Deletion of two downstream genes alters expression of the hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough.

    abstract::The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Keon RG,Fu R,Voordouw G

    更新日期:1997-06-01 00:00:00

  • Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2.

    abstract::A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were reco...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pfeiffer P,Radler F

    更新日期:1984-04-01 00:00:00

  • [Diurnal progress of NADP-linked glyceraldehyde-3-phosphate-dehydrogenase in synchronous culture of unicellular green alga Ankistrodesmus braunii and its susceptibility to X-irradiation and inhibitiors of protein synthesis].

    abstract::1. The daily progress of NADP-linked GPD-activity rise in synchronous culture of Ankistrodesmus braunii was investigated in respect to short time increase of activity by light. After various exposure times cells were temporarily deprived of light and subsequently the so-called dark value as well as the light value (da...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Theiss-Seuberling HB

    更新日期:1975-06-22 00:00:00

  • Expression of the bifunctional Bacillus subtilis TatAd protein in Escherichia coli reveals distinct TatA/B-family and TatB-specific domains.

    abstract::In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Barnett JP,Lawrence J,Mendel S,Robinson C

    更新日期:2011-08-01 00:00:00

  • Increased mRNA expression of interferon-induced Mx1 and immunomodulation following oral administration of IFN-alpha2b-transformed B. longum to mice.

    abstract::We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Yu Z,Zeng Z,Huang Z,Lian J,Yang J,Deng Q,Zeng W

    更新日期:2010-08-01 00:00:00

  • The correlation of the gene csoS2 of the carboxysome operon with two polypeptides of the carboxysome in thiobacillus neapolitanus

    abstract::The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Baker SH,Lorbach SC,Rodriguez-Buey M,Williams DS,Aldrich HC,Shively JM

    更新日期:1999-10-01 00:00:00

  • Characterization and synthesis regulation of Penicillium italicum 1,6-beta-glucanase.

    abstract::The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Santos T,Nombela C,Villanueva JR,Larriba G

    更新日期:1979-06-01 00:00:00

  • Control of pyrimidine nucleotide formation in Pseudomonas aurantiaca.

    abstract::The control of pyrimidine nucleotide formation in the bacterium Pseudomonas aurantiaca ATCC 33663 by pyrimidines was studied. The activities of the pyrimidine biosynthetic pathway enzymes were investigated in P. aurantiaca ATCC 33663 cells and from cells of an auxotroph lacking orotate phosphoribosyltransferase activi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Domakonda A,West TP

    更新日期:2020-08-01 00:00:00

  • Diaphorobacter ruginosibacter sp. nov., isolated from soybean root nodule, and emended description of the genus Diaphorobacter.

    abstract::A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Wei XL,Han MS,Xia CC,Ding SL,Xu L,Lin YB,Wang L,Wei GH

    更新日期:2015-06-01 00:00:00

  • Influence of growth phase and carbon source on the content of rubredoxin in Acinetobacter calcoaceticus.

    abstract::The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Claus R,Asperger O,Kleber HP

    更新日期:1980-12-01 00:00:00

  • Direct studies of dikaryotization in Schizophyllum commune. II. Behavior and fate of multikaryotic hyphase.

    abstract::The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Niederpruem DJ

    更新日期:1980-12-01 00:00:00

  • The early formation of the photosynthetic apparatus in Rhodospirillum rubrum.

    abstract::The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Oelze J,Pahike W

    更新日期:1976-07-01 00:00:00

  • Characteration of RNA synthesized during germination of Blastocladia ramosa Zoospores.

    abstract::The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Jaworski AJ,Torzilli AP

    更新日期:1975-12-31 00:00:00

  • Environmental regulation of alcohol metabolism in thermotolerant methylotrophic Bacillus strains.

    abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Arfman N,de Vries KJ,Moezelaar HR,Attwood MM,Robinson GK,van Geel M,Dijkhuizen L

    更新日期:1992-01-01 00:00:00

  • Strand-specific RNA-seq analysis of the Acidithiobacillus ferrooxidans transcriptome in response to magnesium stress.

    abstract::Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Tang D,Duan J,Gao Q,Zhao Y,Li Y,Chen P,Zhou J,Wu Z,Xu R,Li H

    更新日期:2018-09-01 00:00:00

  • Elucidation of the pathways of catabolic glutamate conversion in three thermophilic anaerobic bacteria.

    abstract::The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Plugge CM,van Leeuwen JM,Hummelen T,Balk M,Stams AJ

    更新日期:2001-07-01 00:00:00

  • Acetate thiokinase and the assimilation of acetate in methanobacterium thermoautotrophicum.

    abstract::Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC; Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC and phosphotransace...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Oberlies G,Fuchs G,Thauer RK

    更新日期:1980-12-01 00:00:00

  • Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province.

    abstract::A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Liu L,Shi S,Liang L,Xu L,Chi M,Zhang X,Li L

    更新日期:2020-04-01 00:00:00

  • Morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces.

    abstract::In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Eppard M,Krumbein WE,Koch C,Rhiel E,Staley JT,Stackebrandt E

    更新日期:1996-07-01 00:00:00

  • Alpha-mating-type-specific suppression and a-mating-type-specific enhancement by tunicamycin of sexual agglutinability in the yeast Saccharomyces cerevisiae.

    abstract::Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hasegawa S,Yanagishima N

    更新日期:1984-08-01 00:00:00

  • Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway.

    abstract::A novel pathway for 2-aminophenol metabolism by Pseudomonas sp. AP-3 is proposed. The proposed pathway is similar to that known for meta-cleavage of catechol except that one of the hydroxyl groups on the metabolites is replaced by an amino group. During the degradation of 2-aminophenol, 2-amino-2,4-pentadienoic acid i...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Takenaka S,Murakami S,Shinke R,Aoki K

    更新日期:1998-08-01 00:00:00

  • Exopolysaccharide biosynthesis is important for Mesorhizobium tianshanense: plant host interaction.

    abstract::Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Wang P,Zhong Z,Zhou J,Cai T,Zhu J

    更新日期:2008-05-01 00:00:00

  • Role of hydrogen sulfide in mercury resistance determined by plasmid of Clostridium cochlearium T-2.

    abstract::Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pan-Hou HS,Imura N

    更新日期:1981-03-01 00:00:00

  • Lactate dehydrogenases in cyanobacteria.

    abstract::NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sanchez JJ,Palleroni NJ,Doudoroff M

    更新日期:1975-06-20 00:00:00

  • Starvation survivability of Thermococcus strains isolated from Japanese oil reservoirs.

    abstract::The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Takahata Y,Hoaki T,Maruyama T

    更新日期:2001-10-01 00:00:00

  • Natural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA.

    abstract::In a soil/sediment model system we have shown recently that a gram-positive bacterium with natural competence (Bacillus subtilis) can take up transforming DNA adsorbed to sand minerals. Here we examined whether also a naturally transformable soil bacterium of the gram-negative pseudomonad (Pseudomonas stutzeri) can be...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Lorenz MG,Wackernagel W

    更新日期:1990-01-01 00:00:00

  • PknE, a serine/threonine protein kinase from Mycobacterium tuberculosis has a role in adaptive responses.

    abstract::Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Kumar D,Palaniyandi K,Challu VK,Kumar P,Narayanan S

    更新日期:2013-01-01 00:00:00

  • The response of Serratia marcescens JG to environmental changes by quorum sensing system.

    abstract::Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sun SJ,Liu HJ,Weng CH,Lai CF,Ai LY,Liu YC,Zhu H

    更新日期:2016-08-01 00:00:00