Abstract：
:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be one of the rate-limiting steps in the overall PCB-degradation process. Three hybrid bacteria constructed to have the ability to completely mineralise 2-, 3-, or 4-monochlorobiphenyl respectively, have been studied to establish the range of mono- and diCBAs that can be utilised. The three strains were able to mineralise one or more of the following CBAs: 2-, 3-, and 4-monochlorobenzoate and 3,5-dichlorobenzoate. No utilisation of 2,3-, 2,5-, 2,6-, or 3,4-diCBA was observed, and only a low concentration (0.11 mM) of 2,4-diCBA was mineralised. When the strain with the widest substrate range (Burkholderia cepacia JHR22) was simultaneously supplied with two CBAs, one that it could utilise plus one that it was unable to utilise, inhibitory effects were observed. The utilisation of 2-CBA (2.5 mM) by this strain was inhibited by 2,3-CBA (200 microM) and 3,4-CBA (50 microM). Although 2,5-cba and 2,6-cba were not utilised as carbon sources by strain jhr22, they did not inhibit 2-cba utilisation at the concentrations studied, whereas 2,4-cba was co-metabolised with 2-cba. The utilisation of 2-, 3-, and 4-chlorobiphenyl by strain JHR22 was also inhibited by the presence of 2,3- or 3,4-diCBA. We conclude that the effect of the formation of toxic intermediates is an important consideration when designing remediation strategies.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Stratford J,Wright MA,Reineke W,Mokross H,Havel J,Knowles CJ,Robinson GKdoi
10.1007/BF01692864subject
Has Abstractpub_date
1996-03-01 00:00:00pages
213-8issue
3eissn
0302-8933issn
1432-072Xjournal_volume
165pub_type
杂志文章abstract：:YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0494-7
更新日期：2009-08-01 00:00:00
abstract：:A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0384-0
更新日期：2002-03-01 00:00:00
abstract：:The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050458
更新日期：1997-06-01 00:00:00
abstract：:A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were reco...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410734
更新日期：1984-04-01 00:00:00
abstract：:1. The daily progress of NADP-linked GPD-activity rise in synchronous culture of Ankistrodesmus braunii was investigated in respect to short time increase of activity by light. After various exposure times cells were temporarily deprived of light and subsequently the so-called dark value as well as the light value (da...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-06-22 00:00:00
abstract：:In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0699-4
更新日期：2011-08-01 00:00:00
abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
abstract：:The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050765
更新日期：1999-10-01 00:00:00
abstract：:The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
abstract：:The control of pyrimidine nucleotide formation in the bacterium Pseudomonas aurantiaca ATCC 33663 by pyrimidines was studied. The activities of the pyrimidine biosynthetic pathway enzymes were investigated in P. aurantiaca ATCC 33663 cells and from cells of an auxotroph lacking orotate phosphoribosyltransferase activi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01842-x
更新日期：2020-08-01 00:00:00
abstract：:A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1102-7
更新日期：2015-06-01 00:00:00
abstract：:The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406170
更新日期：1980-12-01 00:00:00
abstract：:The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406155
更新日期：1980-12-01 00:00:00
abstract：:The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454853
更新日期：1976-07-01 00:00:00
abstract：:The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
abstract：:The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245161
更新日期：1992-01-01 00:00:00
abstract：:Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1503-5
更新日期：2018-09-01 00:00:00
abstract：:The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100286
更新日期：2001-07-01 00:00:00
abstract：:Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransace...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406167
更新日期：1980-12-01 00:00:00
abstract：:A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01774-1
更新日期：2020-04-01 00:00:00
abstract：:In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050350
更新日期：1996-07-01 00:00:00
abstract：:Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410896
更新日期：1984-08-01 00:00:00
abstract：:A novel pathway for 2-aminophenol metabolism by Pseudomonas sp. AP-3 is proposed. The proposed pathway is similar to that known for meta-cleavage of catechol except that one of the hydroxyl groups on the metabolites is replaced by an amino group. During the degradation of 2-aminophenol, 2-amino-2,4-pentadienoic acid i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050624
更新日期：1998-08-01 00:00:00
abstract：:Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0345-3
更新日期：2008-05-01 00:00:00
abstract：:Mercury resistance of Clostridium cochlearium T-2P was found to be controlled by a different mechanism from those reported so far since no mercury-reducing activity was detected in this strain. The H2S generating ability as well as the demethylating activity in this bacterium was eliminated by the treatment of the cur...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00417179
更新日期：1981-03-01 00:00:00
abstract：:NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447300
更新日期：1975-06-20 00:00:00
abstract：:The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100318
更新日期：2001-10-01 00:00:00
abstract：:In a soil/sediment model system we have shown recently that a gram-positive bacterium with natural competence (Bacillus subtilis) can take up transforming DNA adsorbed to sand minerals. Here we examined whether also a naturally transformable soil bacterium of the gram-negative pseudomonad (Pseudomonas stutzeri) can be...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00276535
更新日期：1990-01-01 00:00:00
abstract：:Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0848-4
更新日期：2013-01-01 00:00:00
abstract：:Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1213-9
更新日期：2016-08-01 00:00:00
