Abstract：
:In this study, on evaluating the physiological function and mechanism of putrescine, we found that putrescine supplementation (1 mM) increases transcription of the putrescine aminotransferase gene, ygjG. Putrescine-dependent expression was confirmed by measuring β-galactosidase activity and with reverse transcription-polymerase chain reaction. To understand the role of putrescine in ygjG expression, we genetically characterized and found that a knockout mutation in an alternative sigma factor, rpoS, abolished putrescine-dependent ygjG-lacZ expression. In the rpoS mutant, RpoS overexpression complemented the mutant phenotype. However, RpoS overexpression induced ygjG-lacZ expression with putrescine supplementation but not without supplementation. We also found that the loss of putrescine-dependent ygjG-lacZ expression induced by rpoS was completely restored under nitrogen-starvation conditions. The putrescine-dependent expression of ygjG-lacZ under this condition was clearly dependent on another alternative sigma factor, rpoN, and its cognate activator ntrC. These results show that rpoS is required for putrescine-dependent ygjG-lacZ expression, but the effect of putrescine on this expression is not caused by simple modulation of RpoS synthesis. Putrescine-dependent expression of ygjG-lacZ was controlled by at least two sigma factors: rpoS under excess nitrogen conditions and rpoN under nitrogen-starvation conditions. These results suggest that putrescine plays an important role in the nitrogen regulation system.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Kim YS,Shin HC,Lee JHdoi
10.1007/s00203-014-0991-1subject
Has Abstractpub_date
2014-09-01 00:00:00pages
611-8issue
9eissn
0302-8933issn
1432-072Xjournal_volume
196pub_type
杂志文章abstract：:Differences in the rumen bacterial community have been previously reported for Soay sheep housed under different day length conditions. This study extends this previous investigation to other organs of the digestive tract, as well as the analysis of ciliated protozoa and anaerobic fungi. The detectable concentrations ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01652-w
更新日期：2019-09-01 00:00:00
abstract：:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288947
更新日期：1994-01-01 00:00:00
abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413098
更新日期：1977-04-01 00:00:00
abstract：:The role of HoxX in hydrogenase biosynthesis of Alcaligenes eutrophus H16 was re-examined. The previously characterized hoxX deletion mutant HF344 and a newly constructed second hoxX mutant carrying a smaller in-frame deletion were studied. The second mutant was impaired in the activity of both the soluble and the mem...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050667
更新日期：1998-11-01 00:00:00
abstract：:Of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. Thirteen soil microorganisms were selected to study the utilization of lindane. Lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachlo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454850
更新日期：1976-07-01 00:00:00
abstract：:Synthetic biology, the design and synthesis of synthetic biological systems from DNA to whole cells, has provided us with the ultimate tools for space exploration and colonisation. Herein, we explore some of the most significant advances and future prospects in the field of synthetic biology, in the context of astrobi...
journal_title：Archives of microbiology
pub_type： 信件
doi：10.1007/s00203-019-01651-x
更新日期：2019-08-01 00:00:00
abstract：:Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1415-9
更新日期：2018-01-01 00:00:00
abstract：:A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436334
更新日期：1975-03-12 00:00:00
abstract：:BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050688
更新日期：1999-01-01 00:00:00
abstract：:Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphog...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1576-1
更新日期：2019-01-01 00:00:00
abstract：:Fungicidic Bacillus amyloliquefaciens strains isolated from the indoor environment of moisture-damaged buildings contained heat-stable, methanol-soluble substances that inhibited motility of boar spermatozoa within 15 min of exposure and killed feline lung cells in high dilution in 1 day. Boar sperm cells lost motilit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0660-x
更新日期：2004-04-01 00:00:00
abstract：:The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0667-4
更新日期：2011-04-01 00:00:00
abstract：:Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245225
更新日期：1990-01-01 00:00:00
abstract：:Light-limited batch cultures of Synechococcus spp. strains PCC 6301 and WH 8103 exhibited a positive correlation between the specific growth rate and the cellular content of both RNA and DNA. The ratio of RNA to DNA increased with the growth rate in Synechococcus sp. strain WH 8103, as it does in heterotrophic bacteri...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050634
更新日期：1998-09-01 00:00:00
abstract：:Thermotoga hypogea is an extremely thermophilic anaerobic bacterium capable of growing at 90 degrees C. It uses carbohydrates and peptides as carbon and energy sources to produce acetate, CO(2), H(2), L-alanine and ethanol as end products. Alcohol dehydrogenase activity was found to be present in the soluble fraction ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0217-x
更新日期：2007-06-01 00:00:00
abstract：:Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050545
更新日期：1998-01-01 00:00:00
abstract：:Microorganisms have evolved themselves to thrive under various extreme environmental conditions such as extremely high or low temperature, alkalinity, and salinity. These microorganisms adapted several metabolic processes to survive and reproduce efficiently under such extreme environments. As the major proportion of ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-018-1602-3
更新日期：2019-01-01 00:00:00
abstract：:A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050516
更新日期：1997-11-01 00:00:00
abstract：:Dunaliella tertiolecta (marine) and D. viridis (halophilic) were each trained by serial transfer to grow at salt concentrations previously regarded as the other's domain. D. viridis then had a salt optimum at 1.0-1.5 M sodium chloride whereas that for D. tertiolecta was less than 0-2 M. Nevertheless D. tertiolecta gre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428592
更新日期：1977-05-13 00:00:00
abstract：:Two bacterial isolates from Great Bay Estuary, New Hampshire, in co-culture carried out anaerobic dissimilation of citric acid with Fe(III) as the terminal electron acceptor. Neither isolate oxidized citrate with Fe(III) anaerobically in axenic culture. The Fe(III) reducer, Shewanella alga strain BrY, did not grow ana...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050383
更新日期：1996-10-17 00:00:00
abstract：:Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0345-3
更新日期：2008-05-01 00:00:00
abstract：:Fifty isolates belonging to the six validly described species of the genus Thermus (T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus, T. brockianus, and T. oshimai) isolated from hot springs of different geographical areas were compared using macrorestriction analysis of genomic DNA and ribotyping. With th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050474
更新日期：1997-08-01 00:00:00
abstract：:The molecular weight of the protein moiety of a phospholipoprotein complex isolated from Pseudomonas facilis has been examined with a variety of sodium dodecylsulfate-polyacrylamide gel electrophoretic systems. A molecular weight of 35 000 was determined for the protein in all analyses. A 35 000-dalton protein was pre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427842
更新日期：1977-10-24 00:00:00
abstract：:The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406155
更新日期：1980-12-01 00:00:00
abstract：:Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050589
更新日期：1998-05-01 00:00:00
abstract：:The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respira...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050548
更新日期：1998-02-01 00:00:00
abstract：:To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0582-8
更新日期：2010-07-01 00:00:00
abstract：:In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01801-1
更新日期：2020-05-01 00:00:00
abstract：:The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4 degrees C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0246-5
更新日期：2007-09-01 00:00:00
abstract：:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF01692864
更新日期：1996-03-01 00:00:00
