Abstract：
:Salt-sensitive mutants of Synechocystis were obtained by random cartridge mutagenesis, and one mutant (mutant 4) was characterized in detail. The salt tolerance of mutant 4 was reduced to about 20% of that of the wild-type. This was caused by a defect in the biosynthetic pathway of the osmoprotective compound glucosylglycerol (GG). Salt-treated cells of mutant 4 accumulated the intermediate glucosylglycerol-phosphate (GG-P). Only low levels of phosphate-free GG were detected. The phosphorylated form of GG was not osmoprotective and seemed to be toxic. In vitro enzyme assays revealed that GG-P-phosphatase activity was completely absent in mutant 4, while GG-P-synthase remained unchanged. The integration site of the aphII cartridge in mutant 4 and the corresponding wild-type region was cloned and sequenced. Mutant 4 was complemented to salt resistance after transformation by the cloned wild-type region. The integration of the cartridge led to a deletion of about 1.1 kb of the chromosomal DNA. This affected two of the identified putative protein coding regions, orfII and stpA. The ORFII protein shows a high degree of similarity to the receiver domain of response regulator proteins. Related sequences were not found for StpA. We assume that in mutant 4, regulatory genes necessary for the process of salt adaptation in Synechocystis are impaired.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hagemann M,Richter S,Zuther E,Schoor Adoi
10.1007/s002030050360subject
Has Abstractpub_date
1996-08-01 00:00:00pages
83-91issue
2eissn
0302-8933issn
1432-072Xjournal_volume
166pub_type
杂志文章abstract：:The spectroscopic properties of stigma inside green and dark-grown cells and of isolated stigma globules have been studied by means of a microspectrophotometer build in the Laboratory. On the base of these results and of the analysis of the absorption spectra of a stigma suspension, cell suspension and the cell methan...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446551
更新日期：1976-12-01 00:00:00
abstract：:Bacterial pathogens are a major threat to both humans and animals worldwide. It is crucial to understand the mechanisms of various disease processes at the molecular level. Shewanella species are widespread in the environment and some are considered as emerging opportunistic human and marine mammal pathogens. In this ...
journal_title：Archives of microbiology
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doi：10.1007/s00203-020-02060-1
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abstract：:The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00404204
更新日期：1995-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248617
更新日期：1991-01-01 00:00:00
abstract：:The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0462-y
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更新日期：2009-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447335
更新日期：1975-08-28 00:00:00
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doi：10.1007/s00203-017-1420-z
更新日期：2017-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00412799
更新日期：1986-01-01 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/s00203-012-0824-z
更新日期：2012-11-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0991-1
更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-004-0660-x
更新日期：2004-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02133-1
更新日期：2020-12-15 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01961-5
更新日期：2020-11-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425219
更新日期：1983-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413014
更新日期：1984-06-01 00:00:00
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更新日期：2021-01-04 00:00:00
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pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427842
更新日期：1977-10-24 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0378-y
更新日期：2002-02-01 00:00:00
abstract：:Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicellular species, Coccochloris elabens (strain 7003), and in a fi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429338
更新日期：1977-03-01 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407938
更新日期：1983-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288947
更新日期：1994-01-01 00:00:00
abstract：:The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0301-2
更新日期：2008-02-01 00:00:00
abstract：:A novel pathway for 2-aminophenol metabolism by Pseudomonas sp. AP-3 is proposed. The proposed pathway is similar to that known for meta-cleavage of catechol except that one of the hydroxyl groups on the metabolites is replaced by an amino group. During the degradation of 2-aminophenol, 2-amino-2,4-pentadienoic acid i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050624
更新日期：1998-08-01 00:00:00
