Characterization and localization of phosphatidylglycerophosphate and phosphatidylserine synthases in Rhodobacter sphaeroides.

Abstract:

:Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km values of PGP synthase were 90 microM for sn-glycerol-3-phosphate and 60 microM for CDP-diacylglycerol; the apparent Km of PS synthase for L-serine was near 165 microM. Both enzymes required Triton X-100 with optimal PS synthase activity at a detergent/CDP-diacylglycerol (mol/mol) ratio of 7.5:1.0, while for optimal PGP synthase, a range of 10-50:1.0 was observed. Unlike the enzyme in Escherichia coli and several other Gram-negative bacteria, the PS synthase activity had a specific requirement for magnesium and was tightly associated with membranes rather than ribosomes in crude cell extracts. Sedimentation studies suggested that the PGP synthase was distributed uniformly over the CM in both chemoheterotrophically and photoheterotrophically grown cells, while the PS synthase was confined mainly to a vesicular CM fraction. Solubilized PGP synthase activity migrated as a single band with a pI value near 5.5 in a chromato-focusing column and 5.8 on isoelectric focusing; in the latter procedure, the pI was shifted to 5.3 in the presence of CDP-diacylglycerol. The PGP synthase activity gave rise to a single polypeptide band in lithium dodecyl sulfate-polyacrylamide gel electrophoresis at 4 degrees C.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Radcliffe CW,Steiner FX,Carman GM,Niederman RA

doi

10.1007/BF00456090

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

132-7

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

152

pub_type

杂志文章
  • A mechanistic study on SMOB-ADP1: an NADH:flavin oxidoreductase of the two-component styrene monooxygenase of Acinetobacter baylyi ADP1.

    abstract::Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-1022-y

    authors: Gröning JA,Kaschabek SR,Schlömann M,Tischler D

    更新日期:2014-12-01 00:00:00

  • Expression of hydrogenase in Hupc strains of Bradyrhizobium japonicum.

    abstract::Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00258144

    authors: Kim H,Gabel C,Maier RJ

    更新日期:1993-01-01 00:00:00

  • The influence of the culture pH value on the direct glucose oxidative pathway in Klebsiella pneumoniae NCTC 418.

    abstract::Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00413140

    authors: Hommes RW,Postma PW,Tempest DW,Neijssel OM

    更新日期:1989-01-01 00:00:00

  • On the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by Peptostreptococcus anaerobius.

    abstract::Enoate reductase present in Clostridium kluyveri and Clostridium spec. La 1 could be detected in three strains of C. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. In C. pasteurianum, C. butyricum and C. propionicum enoate reductase could...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00419482

    authors: Giesel H,Simon H

    更新日期:1983-08-01 00:00:00

  • Virulent strains of Salmonella enteritidis disrupt the epithelial barrier of Caco-2 and HEp-2 cells.

    abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030000236

    authors: Solano C,Sesma B,Alvarez M,Urdaneta E,Garcia-Ros D,Calvo A,Gamazo C

    更新日期:2001-01-01 00:00:00

  • NOX1/2 activation in human gingival fibroblasts by Fusobacterium nucleatum facilitates attachment of Porphyromonas gingivalis.

    abstract::Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1223-7

    authors: Ahn SH,Song JE,Kim S,Cho SH,Lim YK,Kook JK,Kook MS,Lee TH

    更新日期:2016-08-01 00:00:00

  • Improved multiplex PCR primers for rapid identification of coagulase-negative staphylococci.

    abstract::Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-017-1415-9

    authors: Kim J,Hong J,Lim JA,Heu S,Roh E

    更新日期:2018-01-01 00:00:00

  • Ethanol induces calcium influx via the Cch1-Mid1 transporter in Saccharomyces cerevisiae.

    abstract::Yeast suffers from a variety of environmental stresses, such as osmotic pressure and ethanol produced during fermentation. Since calcium ions are protective for high concentrations of ethanol, we investigated whether Ca(2+) flux occurs in response to ethanol stress. We find that exposure of yeast to ethanol induces a ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-010-0673-6

    authors: Courchesne WE,Vlasek C,Klukovich R,Coffee S

    更新日期:2011-05-01 00:00:00

  • The P-type ATPase CtpG preferentially transports Cd2+ across the Mycobacterium tuberculosis plasma membrane.

    abstract::P1B-type ATPases are involved in heavy metal transport across the plasma membrane. Some Mycobacterium tuberculosis P-type ATPases are induced during infection, suggesting that this type of transporter could play a critical role in mycobacterial survival. To date, the ion specificity of M. tuberculosis heavy metal-tran...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-017-1465-z

    authors: López M,Quitian LV,Calderón MN,Soto CY

    更新日期:2018-04-01 00:00:00

  • Localization and activities of nitrogenase, glutamine synthetase and glutamate synthase in Azotobacter vinelandii grown in oxygen-controlled continuous culture.

    abstract::Azotobacter vinelandii was grown in oxygen-controlled continuous cultures under conditions of dinitrogen fixation. Different oxygen concentrations were adjusted with air. Cell-free extracts were employed to study the oxygen dependency of the intracellular distribution and activity of the following enzymes: nitrogenase...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00415614

    authors: Röckel D,Hernando JJ,Vakalopoulou E,Post E,Oelze J

    更新日期:1983-10-01 00:00:00

  • Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis.

    abstract::The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-008-0366-6

    authors: Olszewski M,Mickiewicz M,Kur J

    更新日期:2008-07-01 00:00:00

  • Unicellular cyanobacteria with a new mode of life: the lack of photosynthetic oxygen evolution allows nitrogen fixation to proceed.

    abstract::Some unicellular N(2)-fixing cyanobacteria have recently been found to lack a functional photosystem II of photosynthesis. Such organisms, provisionally termed UCYN-A, of the oceanic picoplanktion are major contributors to the global marine N-input by N(2)-fixation. Since their photosystem II is inactive, they can per...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00203-010-0621-5

    authors: Bothe H,Tripp HJ,Zehr JP

    更新日期:2010-10-01 00:00:00

  • Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi.

    abstract::Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivati...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00436357

    authors: Loppes R,Deltour R

    更新日期:1975-05-05 00:00:00

  • Morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces.

    abstract::In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050350

    authors: Eppard M,Krumbein WE,Koch C,Rhiel E,Staley JT,Stackebrandt E

    更新日期:1996-07-01 00:00:00

  • A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes.

    abstract::A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease cou...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-006-0112-x

    authors: Niu Q,Huang X,Zhang L,Li Y,Li J,Yang J,Zhang K

    更新日期:2006-06-01 00:00:00

  • Analysis of cell growth dynamics of Pediococcus acidilactici in the presence of inulin in an optimized microenvironment.

    abstract::The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected for optimization study using respo...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-015-1131-2

    authors: Samanta Koruri S,Chowdhury R,Bhattacharya P

    更新日期:2015-09-01 00:00:00

  • The occurrence of teichoic acids in streptomycetes.

    abstract::The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00421893

    authors: Naumova IB,Kuznetsov VD,Kudrina KS,Bezzubenkova AP

    更新日期:1980-05-01 00:00:00

  • [The influence of the antivitamine pyrithiamine on the metabolism of the thiamin-autotrophic Serratia marcescens].

    abstract::A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to p...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00436334

    authors: Hagedorn H,Schmidt OR

    更新日期:1975-03-12 00:00:00

  • Quinolinate dehydrogenase and 6-hydroxyquinolinate decarboxylase involved in the conversion of quinolinic acid to 6-hydroxypicolinic acid by Alcaligenes sp. strain UK21.

    abstract::In the conversion of quinolinic acid to 6-hydroxypicolinic acid by whole cells of Alcaligenes sp. strain UK21, the enzyme reactions involved in the hydroxylation and decarboxylation of quinolinic acid were examined. Quinolinate dehydrogenase, which catalyzes the first step, the hydroxylation of quinolinic acid, was so...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0573-0

    authors: Uchida A,Ogawa M,Yoshida T,Nagasawa T

    更新日期:2003-08-01 00:00:00

  • Effect of cra gene knockout together with edd and iclR genes knockout on the metabolism in Escherichia coli.

    abstract::To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-008-0406-2

    authors: Sarkar D,Siddiquee KA,Araúzo-Bravo MJ,Oba T,Shimizu K

    更新日期:2008-11-01 00:00:00

  • Comparative immunological study of catalases in the genus Micrococcus.

    abstract::Double immunodiffusion tests were performed with crude extracts from various Micrococcus species and antisera against catalase of Micrococcus luteus CCM 169. Cell-free extracts of M. lylae ATCC 27566 exhibited good cross-reaction. Cell-free extracts or catalase enriched preparations of M. varians reacted very weakly a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00429631

    authors: Rupprecht M,Schleifer KH

    更新日期:1977-07-26 00:00:00

  • Gene cloning, expression and functional characterization of an acyl carrier protein AcpV from Vibrio anguillarum.

    abstract::Acyl carrier protein (ACP) is a small acidic protein that acts as an essential cofactor in many biosynthetic pathways depending on acyl transfer reactions. In this work, a Vibrio anguillarum ACP encoding gene, acpV, was first cloned from the chromosome of a virulent V. anguillarum strain MVM425. acpV was over-expresse...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-005-0058-4

    authors: Liu Q,Ma Y,Zhou L,Zhang Y

    更新日期:2006-03-01 00:00:00

  • Biosynthetic Pathways of Vibrio succinogenes growing with fumarate as terminal electron acceptor and sole carbon source.

    abstract::1. With fumarate as the terminal electron acceptor and either H2 or formate as donor, Vibrio succinogenes could grow anaerobically in a mineral medium using fumarate as the sole carbon source. Both the growth rate and the cell yield were increased when glutamate was also present in the medium. 2. Glutamate was incorpo...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00405882

    authors: Bronder M,Mell H,Stupperich E,Kröger A

    更新日期:1982-05-01 00:00:00

  • Role of VcrD1 protein in expression and secretion of flagellar components in Vibrio parahaemolyticus.

    abstract::VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-1069-9

    authors: Noh HJ,Nagami S,Kim MJ,Kim J,Lee NK,Lee KH,Park SJ

    更新日期:2015-04-01 00:00:00

  • Transcription in the prpC-yloQ region in Bacillus subtilis.

    abstract::The prpC and prkC genes encode two proteins with opposing activities, eukaryotic-like protein phosphatase and protein kinase involved in the sporulation and biofilm formation processes. The prpC gene overlaps 3 bp of the prkC gene; prkC is followed by the essential gene, yloQ. The organisation of the prpC, prkC and yl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-005-0015-2

    authors: Iwanicki A,Hinc K,Seror S,Wegrzyn G,Obuchowski M

    更新日期:2005-09-01 00:00:00

  • Genome-wide transcriptional changes during the lag phase of Saccharomyces cerevisiae.

    abstract::The set of physiological and metabolic changes occurring immediately after inoculation and during the lag phase is thought to be of vital importance for optimal offset of fermentation. The transcriptional changes taking place during the lag phase after inoculation of a late-respiratory-phase yeast culture into fresh, ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0527-6

    authors: Brejning J,Jespersen L,Arneborg N

    更新日期:2003-04-01 00:00:00

  • Purification, partial characterization, and subcellular localization of a 38 kilodalton, calcium-regulated protein of Rhizobium fredii USDA208.

    abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00248480

    authors: Krishman HB,Pueppke SG

    更新日期:1993-01-01 00:00:00

  • Pharmacological properties of some mangrove sediment-associated bacillus isolates.

    abstract::Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01999-5

    authors: Rajan L,Chakraborty K,Chakraborty RD

    更新日期:2021-01-01 00:00:00

  • Cloning and characterization of two novel DNases from Streptococcus pyogenes.

    abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-002-0412-8

    authors: Hasegawa T,Torii K,Hashikawa S,Iinuma Y,Ohta M

    更新日期:2002-06-01 00:00:00

  • Molecular identification of clinical and environmental avian Aspergillus isolates.

    abstract::Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-019-01618-y

    authors: Sabino R,Burco J,Valente J,Veríssimo C,Clemons KV,Stevens DA,Tell LA

    更新日期:2019-03-01 00:00:00