Identification of 4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ.

Abstract:

:The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produce lipopeptides fengycin and surfactin in accordance with our previous report. In this study, another antimicrobial substance was separated and purified from the culture supernatant of strain fmbJ using the silica gel column chromatography and preparative reversed-phase high-performance liquid chromatography. By means of electrospray ionization mass spectroscopy, infrared spectroscopy, and nuclear magnetic resonance, the antagonistic compound was determined to be 4″-isovaleryl-spiramycin III with the molecular weight of 982 Da. This report is the first to introduce the finding of spiramycin produced from Bacillus sp. The study provides a novel source for the production of spiramycin in pharmaceutical industries.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Deng Y,Ju Y,Lu Z,Lu F,Yan D,Bie X

doi

10.1007/s00203-013-0939-x

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

87-95

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

196

pub_type

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