Molecular identification of clinical and environmental avian Aspergillus isolates.

Abstract:

:Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period, clinical samples were recovered from 44 birds with clinical signs of the disease, clinical pathology results suspicious of aspergillosis, or from birds that died from Aspergillus spp. infection. Environmental sampling was also performed in seabird rehabilitation centers and natural seabird environments. Seventy-seven isolates (43 clinical and 34 environmental) were identified as Aspergillus fumigatus sensu stricto. No cryptic species from the Fumigati section were detected. Two environmental isolates were identified as Aspergillus nidulans var. dentatus and Aspergillus spinulosporus. None of the Aspergillus isolates tested were resistant to itraconazole. Our study emphasizes the dominant association of Aspergillus fumigatus sensu stricto in avian mycoses and shows the lack of itraconazole resistance in the studied isolates.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Sabino R,Burco J,Valente J,Veríssimo C,Clemons KV,Stevens DA,Tell LA

doi

10.1007/s00203-019-01618-y

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

253-257

issue

2

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-019-01618-y

journal_volume

201

pub_type

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