Abstract：
:Previous studies have shown that centrosome position and structure can be influenced by actin filaments, that centrosomes can influence actin organization, and that an actin homologue is associated with centrosomes. Such observations suggest the existence of connections between centrosomes and actin networks. In keeping with such observations, we show that the pericentriolar material, a main component of centrosomes, contains vinculin, a well-known component of cell adhesion plaques and of adherens cell junctions. We find that in various cell types, centrosomes are specifically stained by five different anti-vinculin antibodies. In adherent cell lines, these antibodies also stained adhesion plaques, but in thymocytes, a cell type devoid of adhesive structures, such antibodies stained only centrosomes. Isolated centrosomes also reacted with the anti-vinculin antibodies and immunoelectron microscopy showed apparent localization of vinculin in the pericentriolar material. Immunoblot analysis confirmed the presence of vinculin in purified centrosomal protein preparations. In such protein fractions, anti-vinculin antibodies reacted with a single polypeptide with an apparent molecular weight similar to that of vinculin. Stepwise solubilization of centrosomal structures using urea showed that high urea concentrations were required to solubilize centrosomal vinculin, suggesting tight association of vinculin with the pericentriolar material. The identification of vinculin as a component of centrosomes provides a possible molecular basis for interaction between F-actin and centrosomes.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Chevrier V,Paintrand M,Koteliansky V,Block MR,Job Ddoi
10.1006/excr.1995.1245subject
Has Abstractpub_date
1995-08-01 00:00:00pages
399-406issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(85)71245-1journal_volume
219pub_type
杂志文章abstract：:p107 and p130 were originally identified as targets of the transforming domains of viral oncoproteins encoded by small DNA tumor viruses. Together with pRB, the protein product of the retinoblastoma gene (Rb), p107 and p130 represent a family of closely related proteins that play critical roles in the regulation of ce...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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abstract：:Calcium-tolerant myocytes were isolated from adult rat hearts by collagenase perfusion and plated on various substrates in serum-free medium and their adhesion to various extracellular matrix (ECM) components was determined. The myocytes attached readily to dishes coated with collagen type IV (C-IV), laminin (LN), and...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90235-0
更新日期：1984-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1048
更新日期：1994-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90227-9
更新日期：1989-01-01 00:00:00
abstract：:We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. ...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1992-09-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2020-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1988-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-09-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1988-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1988-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1998-09-15 00:00:00
abstract：:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...
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pub_type： 杂志文章
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更新日期：2006-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-02-01 00:00:00
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更新日期：1991-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1995-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1327
更新日期：1993-12-01 00:00:00
abstract：:Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2005-05-15 00:00:00
