Abstract:
:The CDC25Mm gene codes for Ras-guanine nucleotide exchange factors. Four different full-length cDNA clones derived from the same gene and coding for proteins of different sizes that have in common the last 661 amino acids have been isolated from mouse brain. In order to investigate the expression of the products of this gene in different tissues we have prepared two polyclonal antibodies directed toward two different regions of the protein comprised in the last C-terminal 472 amino acids. While in most of the tested tissues we have been unable to definitely identify CDC25Mm products, in NIH3T3 fibroblasts we have found a poorly expressed 120-kDa protein. In the mouse brain we have identified two proteins of 140 and 58 kDa. While the former is expressed in the adult mouse, the latter is present in the embryo and persists for few days after birth. This finding suggests that differential expression of various forms of CDC25Mm may be involved in brain development.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ferrari C,Zippel R,Martegani E,Gnesutta N,Carrera V,Sturani Edoi
10.1006/excr.1994.1048subject
Has Abstractpub_date
1994-02-01 00:00:00pages
353-7issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71048-2journal_volume
210pub_type
杂志文章abstract::The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90709-2
更新日期:1984-01-01 00:00:00
abstract::Apoptosis plays an important role in eliminating dysfunctional damaged cells. For skin, the best characterized injurious environmental agent is ultraviolet (UV) irradiation. Most of the damaging UV irradiation is absorbed in the epidermis and leads to apoptosis of keratinocytes. However, epidermal melanocytes appear t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0143
更新日期:1996-05-01 00:00:00
abstract::We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.05.012
更新日期:2008-08-15 00:00:00
abstract::There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2017.03.034
更新日期:2017-07-15 00:00:00
abstract::Cultured megakaryocytes, isolated from guinea pig bone marrow, were treated with buffer or adenosine diphosphate (ADP) (10 microM) on plain or coated glass surfaces. Control cells were rounded and non-adherent. The nucleotide induced the cells to spread to several times the initial diameter, and to become flattened an...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90649-9
更新日期:1984-06-01 00:00:00
abstract::Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90211-r
更新日期:1990-01-01 00:00:00
abstract::Melanoma formation in the teleost fish Xiphophorus is caused by the uncontrolled activity of the genetically defined tumor locus Tu. The critical component of this locus is the Xmrk oncogene encoding a subclass I receptor tyrosine kinase. Overexpression and constitutive activation of the Xmrk receptor triggers a set o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4470
更新日期:1999-06-15 00:00:00
abstract::Human calmodulin-like protein (CLP) is a calcium-binding protein down-regulated in a cell culture model of mammary tumorigenesis as well as in a majority of breast cancers in vivo. CLP down-regulation may be a result of the poorly differentiated state of these cell lines and tumors, or CLP expression may be incompatib...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5254
更新日期:2001-07-15 00:00:00
abstract::In a previous study, we found that a specific inhibitor of cellular arginine-specific mono(ADP-ribosyl) transferase, meta-iodobenzylguanidine (MIBG), reversibly inhibited both proliferation and differentiation of cultured embryonic chick primary muscle myoblasts. In addition, we observed that arginine-specific ADP-rib...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0213
更新日期:1996-07-10 00:00:00
abstract::Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(02)00028-9
更新日期:2003-02-01 00:00:00
abstract::The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90277-0
更新日期:1985-01-01 00:00:00
abstract::The radula of the limpet, Cellana toreuma, consists of a continuous series of teeth in various stages of iron biomineralization. The major iron-binding protein of the limpet's iron-containing granule (siderosome) has been purified and identified as ferritin. Limpet ferritin has a M(r) of 575 kDa and can be resolved in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1214
更新日期:1995-07-01 00:00:00
abstract::The assumption that cells are temporally organized systems, i.e. showing relevant dynamics of their state variables such as gene expression or protein and metabolite concentration, while tacitly given for granted at the molecular level, is not explicitly taken into account when interpreting biological experimental dat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.09.007
更新日期:2011-12-10 00:00:00
abstract::MyD88, a protein implicated in interleukin-1 signaling, was localized in HeLa cells transiently transfected with an epitope-tagged (flag) version of MyD88. Overexpression of MyD88 can induce apoptosis. We have analyzed the fine structural intracellular distribution of MyD88 using immunoelectron microscopy. MyD88 is lo...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4131
更新日期:1998-08-25 00:00:00
abstract::Prosomes constitute the multicatalytic proteinase (MCP) core of the 26S proteasomes, but were first observed as subcomplexes of untranslated mRNP; this suggests that they play a putative role in the control of protein biosynthesis in addition to their catabolic enzymatic function. In previous investigations it was sho...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3561
更新日期:1997-05-25 00:00:00
abstract::Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.02.020
更新日期:2010-07-15 00:00:00
abstract::The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.10.021
更新日期:2010-02-01 00:00:00
abstract::Drebrin is an actin-binding protein which is expressed at highly levels in neurons. When introduced into fibroblasts, it has been known to bind to F-actin and to cause remodeling of F-actin. Here, we performed a domain analysis of the actin-binding and actin-remodeling activities of drebrin. Various fragments of drebr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4663
更新日期:1999-12-15 00:00:00
abstract::Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3916
更新日期:1998-03-15 00:00:00
abstract::Dictyostelium discoideum cells synthesize and secrete the chemoattractant cAMP within minutes after chemotactic stimulation. During development, this signal-relay process is instrumental in cell aggregation, pattern formation, and differentiation. Cyclic AMP is known to accumulate inside the cell before secretion. In ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90181-x
更新日期:1989-03-01 00:00:00
abstract::Incubation of cultured cells at 20 degrees C blocks the transport of newly synthesized plasma membrane proteins, and the proteins accumulate intracellularly in a terminally glycosylated form. When baby hamster kidney cells are infected with the ts O45 mutant of vesicular stomatitis virus, and incubated at 20 degrees C...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90190-5
更新日期:1988-04-01 00:00:00
abstract::Porcine brain microvascular endothelial cells (PBMEC) were isolated from fresh brains by several enzymatic digestion steps followed by a gradient centrifugation. Cells of the primary culture were transfected with pRNS-1, encoding for the small and large T-antigens of SV 40, by means of lipofection. After selection wit...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0298
更新日期:1996-10-10 00:00:00
abstract::The intracellular compartmentation of Ap4A in various growth and cell-cycle stages in mammalian cells was studied by applying a non-aqueous extraction procedure for cell nuclei. In both slowly and in exponentially growing Ehrlich ascites tumour cells from random cultures, more than 75% of the whole cellular Ap4A conte...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90607-5
更新日期:1986-08-01 00:00:00
abstract::The osteoblast-like MC3T3-E1 cell line provides an excellent in vitro model of bone development. This system undergoes three orderly time-dependent phases characterized by proliferating preosteoblasts, matrix accumulation by postmitotic differentiating osteoblasts, and mineralization of the matrix, which results in th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4879
更新日期:2000-05-25 00:00:00
abstract::A temperature-sensitive mutant, tsJT60, grew exponentially at both 34 degrees and 39.5 degrees C, but when stimulated from the resting state it entered S phase at 34 degrees but not at 39.5 degrees C. The mutated function appeared to be a prerequisite throughout from 0 to 9 h following the stimulation, in order that G...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90543-4
更新日期:1986-07-01 00:00:00
abstract::Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.112368
更新日期:2020-12-15 00:00:00
abstract::Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.09.026
更新日期:2007-01-01 00:00:00
abstract::The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90034-6
更新日期:1992-11-01 00:00:00
abstract::The expression level of DNA polymerase beta was determined in various mouse tissues. Northern blot hybridization analysis using rat cDNA as a probe revealed that the mRNA of about 1.5 kb for this enzyme is present in all kinds of tissues examined, but its content widely varies among tissues; the most abundant DNA poly...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90191-2
更新日期:1989-03-01 00:00:00
abstract::The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent sta...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90423-r
更新日期:1991-12-01 00:00:00