当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Focal adhesion kinase pp125FAK and the beta 1 integrin subunit are constitutively complexed in HaCaT cells.

Focal adhesion kinase pp125FAK and the beta 1 integrin subunit are constitutively complexed in HaCaT cells.

Abstract：

:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possible association of pp125FAK, an adhesion involved tyrosine kinase, with the integrin beta 1 subunit. Further we wanted to know to which extent protein tyrosine phosphorylation affects cell adhesion to the ECM and the possible beta 1 integrin/pp125FAK complex. We were able to show that in HaCaT cells (a human keratinocyte derived cell line) the integrin beta 1 subunit is associated with tyrosine kinase pp125FAK. This association was observed in ECM-adherent cells and nonadherent cells and is independent of tyrosine phosphorylation. However, cell adhesion of HaCaT cells to specific substrates requires tyrosine phosphorylation since genistein treatment that blocks phosphorylation of many cellular proteins as pp125FAK led to a reduced substrate adhesion.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Danker K,Gabriel B,Heidrich C,Reutter W

doi

10.1006/excr.1997.3916

subject

Has Abstract

pub_date

1998-03-15 00:00:00

pages

326-31

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(97)93916-1

journal_volume

239

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Immunological identity of proteins that bind stored 5S RNA in Xenopus oocytes.

    abstract：:In small oocytes of Xenopus laevis, the three most abundant proteins are isolated as basic polypeptides with molecular weights of 48 kD (P48), 43 kD (P43) and 40 kD (P40, also known as transcription factor IIIA). All three proteins share common properties in being able to bind specifically ribosomal 5S RNA molecules a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90602-5

    authors： Barrett P,Johnson RM,Sommerville J

    更新日期：1984-08-01 00:00:00

  • Proteoglycan synthesis by cultured liver endothelium: the role of membrane-associated heparan sulfate in transferrin binding.

    abstract：:Liver endothelium has been reported to possess membrane receptors for the iron-binding protein transferrin (Tf). Similarly, the core protein of proteoglycans (PG) associated with cell membrane in many cell systems can bind Tf. To find out if membrane-associated proteoglycans can explain Tf-binding ability of liver end...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90120-y

    authors： Omoto E,Minguell JJ,Tavassoli M

    更新日期：1990-03-01 00:00:00

  • Antineoplastic activity of Salmonella Typhimurium outer membrane nanovesicles.

    abstract：:Nano-sized Gram-negative bacterial outer membrane vesicles possess unique structural and immunostimulatory effects that could be exploited to regress tumors by alerting the host immune system and reversing the immunosuppressive tumor microenvironment. The current study was conducted to investigate the antitumor activi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112423

    authors： Aly RG,El-Enbaawy MI,Abd El-Rahman SS,Ata NS

    更新日期：2021-02-01 00:00:00

  • A functional role for ERK in gene induction, but not in neurite outgrowth in differentiating neuroblastoma cells.

    abstract：:The human neuroblastoma cell line SH-SY5Y can differentiate into a functional sympathetic neuronal phenotype when treated with low concentrations of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of serum or defined growth factors. When TrkA is introduced into the cells, NGF also induces ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5163

    authors： Olsson AK,Nånberg E

    更新日期：2001-04-15 00:00:00

  • Elimination of mycoplasmas from cell cultures utilizing hyperimmune sera.

    abstract：:Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90502-6

    authors： Jeansson S,Brorson JE

    更新日期：1985-11-01 00:00:00

  • Altered permeability and modulatory character of connexin channels during mammary gland development.

    abstract：:Abrupt developmental changes occur in structural form and function of connexin (Cx) channels in the mouse mammary gland. Microarray study shows that the principal connexin isoform in epithelial cells during pregnancy is Cx26, up-regulated and persisting from the virgin. After parturition, there is rapid induction of C...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.05.003

    authors： Locke D,Stein T,Davies C,Morris J,Harris AL,Evans WH,Monaghan P,Gusterson B

    更新日期：2004-08-15 00:00:00

  • Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation.

    abstract：:We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectiv...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3708

    authors： Bouniol-Baly C,Nguyen E,Besombes D,Debey P

    更新日期：1997-10-10 00:00:00

  • Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90593-7

    authors： Grant NJ,Aimar C,Oriol-Audit C

    更新日期：1984-02-01 00:00:00

  • The role of the Nir/rdgB protein family in membrane trafficking and cytoskeleton remodeling.

    abstract：:The Nir/rdgB family of proteins has been identified in a variety of eukaryotic organisms, ranging from worms to mammals. The Drosophila retinal degeneration B (rdgB), a protein that is required for photoreceptor cell viability and light response, was the first to be identified. It consists an amino-terminal phosphatid...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2004.02.033

    authors： Lev S

    更新日期：2004-07-01 00:00:00

  • Nuclear translocation of a leukocyte elastase Inhibitor/Elastase complex during staurosporine-induced apoptosis: role in the generation of nuclear L-DNase II activity.

    abstract：:Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4737

    authors： Belmokhtar CA,Torriglia A,Counis MF,Courtois Y,Jacquemin-Sablon A,Ségal-Bendirdjian E

    更新日期：2000-01-10 00:00:00

  • Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.

    abstract：:A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90604-9

    authors： Schmidt-Zachmann MS,Hügle B,Scheer U,Franke WW

    更新日期：1984-08-01 00:00:00

  • Myostatin signals through Pax7 to regulate satellite cell self-renewal.

    abstract：:Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.09.012

    authors： McFarlane C,Hennebry A,Thomas M,Plummer E,Ling N,Sharma M,Kambadur R

    更新日期：2008-01-15 00:00:00

  • Deletion of the p107/p130-binding domain of Mip130/LIN-9 bypasses the requirement for CDK4 activity for the dissociation of Mip130/LIN-9 from p107/p130-E2F4 complex.

    abstract：:Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.07.014

    authors： Sandoval R,Pilkinton M,Colamonici OR

    更新日期：2009-10-15 00:00:00

  • Kidins220/ARMS depletion is associated with the neural-to Schwann-like transition in a human neuroblastoma cell line model.

    abstract：:Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.12.027

    authors： Rogers DA,Schor NF

    更新日期：2013-03-10 00:00:00

  • Inorganic phosphate regulates multiple genes during osteoblast differentiation, including Nrf2.

    abstract：:The process of osteoblast differentiation and matrix mineralization requires a rise in alkaline phosphatase enzymatic activity resulting in the generation of free phosphate. The ability of inorganic phosphate to regulate gene transcription and cellular function represents a potentially novel extracellular signaling me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00213-1

    authors： Beck GR Jr,Moran E,Knecht N

    更新日期：2003-08-15 00:00:00

  • Intracellular insulin quantification by cell-ELISA.

    abstract：:Current methods of monitoring insulin in culture are limited to soluble insulin (secretions or lysates) or synthetic gene reporter analyses. We present an insulin-specific cell enzyme-linked immunosorbent assay (cell-ELISA) to assess relative intracellular insulin protein content of adherent cultures, normalized to ce...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.06.014

    authors： Andersen PL,Vermette P

    更新日期：2016-09-10 00:00:00

  • Coronin 3 involvement in F-actin-dependent processes at the cell cortex.

    abstract：:The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.12.015

    authors： Rosentreter A,Hofmann A,Xavier CP,Stumpf M,Noegel AA,Clemen CS

    更新日期：2007-03-10 00:00:00

  • Caloric restriction: conservation of cellular replicative capacity in vitro accompanies life-span extension in mice.

    abstract：:We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1091

    authors： Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

    更新日期：1995-04-01 00:00:00

  • CDK8 regulates the angiogenesis of pancreatic cancer cells in part via the CDK8-β-catenin-KLF2 signal axis.

    abstract：BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angioge...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.05.033

    authors： Wei R,Kong L,Xiao Y,Yuan H,Song Y,Wang J,Yu H,Mao S,Xu W

    更新日期：2018-08-15 00:00:00

  • KDM5B promotes breast cancer cell proliferation and migration via AMPK-mediated lipid metabolism reprogramming.

    abstract：:Lysine demethylase 5B (KDM5B) is up-regulated in many cancers, including breast cancer. However, the underlying metabolic mechanisms of KDM5B on breast cancer progression are poorly understood. Here, we showed that KDM5B expression positively correlates with metastasis in breast cancer. Cell functional analyses were d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.04.006

    authors： Zhang ZG,Zhang HS,Sun HL,Liu HY,Liu MY,Zhou Z

    更新日期：2019-06-15 00:00:00

  • Recruitment of Uev1B to Hrs-containing endosomes and its effect on endosomal trafficking.

    abstract：:Endocytosis of signaling receptors, such as epidermal growth factor receptor (EGFR), tightly controls the signal transduction process triggered by ligand activation of these receptors. To identify new regulators of the endocytic trafficking of EGFR an RNA interference screen was performed for genes involved in ubiquit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.04.017

    authors： Duex JE,Mullins MR,Sorkin A

    更新日期：2010-08-01 00:00:00

  • Mitochondria control acute and chronic responses to hypoxia.

    abstract：:There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2017.03.034

    authors： McElroy GS,Chandel NS

    更新日期：2017-07-15 00:00:00

  • Hsf1 on a leash - controlling the heat shock response by chaperone titration.

    abstract：:Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2020.112246

    authors： Masser AE,Ciccarelli M,Andréasson C

    更新日期：2020-11-01 00:00:00

  • Mitogen-independent phosphorylation of S6K1 and decreased ribosomal S6 phosphorylation in senescent human fibroblasts.

    abstract：:The p70 ribosomal S6 kinase (S6K1) is rapidly activated following growth factor stimulation of quiescent fibroblasts and inhibition of this enzyme results in a G(1) arrest. Phosphorylation of the ribosomal S6 protein by S6K1 regulates the translation of both ribosomal proteins and initiation factors, leading to an inc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4965

    authors： Zhang H,Hoff H,Marinucci T,Cristofalo VJ,Sell C

    更新日期：2000-08-25 00:00:00

  • Type I collagen promotes modulation of cultured rabbit arterial smooth muscle cells from a contractile to a synthetic phenotype.

    abstract：:The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1016

    authors： Yamamoto M,Yamamoto K,Noumura T

    更新日期：1993-01-01 00:00:00

  • Absence of the Shb gene in mixed-lineage leukemia MLL-AF9 cells increases latency in mice despite higher proliferation rates in vitro.

    abstract：:Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112368

    authors： Jamalpour M,Bergquist E,Welsh M

    更新日期：2020-12-15 00:00:00

  • Biochemical studies on lysin, a cell wall degrading enzyme released during fertilization in Chlamydomonas.

    abstract：:New methods have been developed for the purification and characterization of the cell wall degrading enzyme, lysin, which is released into the medium during the mating reaction of the biflagellated alga Chlamydomonas reinhardtii. A quantitative spectrophotometric assay that detects the number of cells losing walls was...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90357-6

    authors： Buchanan MJ,Snell WJ

    更新日期：1988-11-01 00:00:00

  • Involvement of cyclic GMP in the fusion of chick embryonic myoblasts in culture.

    abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90470-s

    authors： Choi SW,Baek MY,Kang MS

    更新日期：1992-03-01 00:00:00

  • Emerging functions: diseases and animal models reshape our view of the cytoskeleton.

    abstract：:Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2004.08.018

    authors： Magin TM,Reichelt J,Hatzfeld M

    更新日期：2004-11-15 00:00:00

  • Identification of vinculin as a pericentriolar component in mammalian cells.

    abstract：:Previous studies have shown that centrosome position and structure can be influenced by actin filaments, that centrosomes can influence actin organization, and that an actin homologue is associated with centrosomes. Such observations suggest the existence of connections between centrosomes and actin networks. In keepi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1245

    authors： Chevrier V,Paintrand M,Koteliansky V,Block MR,Job D

    更新日期：1995-08-01 00:00:00