Abstract：
:Lysine demethylase 5B (KDM5B) is up-regulated in many cancers, including breast cancer. However, the underlying metabolic mechanisms of KDM5B on breast cancer progression are poorly understood. Here, we showed that KDM5B expression positively correlates with metastasis in breast cancer. Cell functional analyses were demonstrated that KDM5B knockdown and KDM5B inhibitor AS-8351 inhibited breast cancer cell proliferation and migration. Furthermore, we reported that KDM5B knockdown and AS-8351 reversed epithelial-mesenchymal transition (EMT) and decreased the protein levels of fatty acid synthase (FASN) and ATP citrate lyase (ACLY) in MCF-7 and MDA-MB-231 cells. Interestingly, we found that activation of AMP-activated protein kinase (AMPK) signaling pathway is involved in KDM5B-mediated EMT and lipid metabolism reprogramming in breast cancer cells. As a result, silencing of KDM5B-induced activation of AMPK signaling pathway inhibited breast cancer cell proliferation and migration. Taken together, our findings indicated that KDM5B was a novel regulator of lipid metabolism reprogramming, and it was suggested a new strategy to treat breast cancer.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Zhang ZG,Zhang HS,Sun HL,Liu HY,Liu MY,Zhou Zdoi
10.1016/j.yexcr.2019.04.006subject
Has Abstractpub_date
2019-06-15 00:00:00pages
182-190issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(19)30156-9journal_volume
379pub_type
杂志文章abstract：:Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1224
更新日期：1994-08-01 00:00:00
abstract：:Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.01.018
更新日期：2013-04-01 00:00:00
abstract：:Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90061-x
更新日期：1989-02-01 00:00:00
abstract：:We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0237
更新日期：1996-08-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives concomitant with changes in morphology and phenotype, reminiscent of an epithelial mesenchymal transition (EMT) [Exp. Cell Res. 252 (1999) 281-291]. In this work, we have isolated the...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.08.015
更新日期：2004-01-01 00:00:00
abstract：:Culture and UV (254 nm) irradiation conditions that are suggested as appropriate for a study of the effect of UV on the limited in vitro lifespan of a normal human diploid fibroblast (HDF) strain are first described. An inoculation density at each subcultivation of 1.8 x 10(4) viable cells/cm-2 permits the decline in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90368-3
更新日期：1978-05-01 00:00:00
abstract：:Cell-CAM 105 is an integral cell surface glycoprotein that is involved in cell-cell adhesion of adult rat hepatocytes in vitro. In the present report we used a radio-immunoassay, a quantitative immunoblotting technique and immunofluorescence microscopy to investigate the expression of cell-CAM 105 in fetal and regener...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90458-1
更新日期：1986-05-01 00:00:00
abstract：:Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90084-9
更新日期：1985-12-01 00:00:00
abstract：:Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migrato...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1288
更新日期：1995-09-01 00:00:00
abstract：:Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respective...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.01.012
更新日期：2012-03-10 00:00:00
abstract：:The proline analogs cis-4-hydroxy-L-proline, 3,4-dehydro-L-proline, and 2-azetidinecarboxylic acid induced increases in the migration rate of retinal capillary endothelial cells, aortal endothelial cells, corneal endothelial cells, aortal smooth muscle cells, and retinal pericytes. cis-4-hydroxy-D-proline did not. The...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90328-x
更新日期：1988-06-01 00:00:00
abstract：:To determine whether the micronucleus makes essential contributions during asexual reproduction, observations were made on cells of Euplotes octocarinatus from which the micronucleus had been removed with a micropipette. Most cells underwent one postenucleation division, then became arrested in macronuclear G1, slowed...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90100-4
更新日期：1985-12-01 00:00:00
abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3876
更新日期：1998-02-25 00:00:00
abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.04.007
更新日期：2006-08-01 00:00:00
abstract：:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90384-1
更新日期：1978-05-01 00:00:00
abstract：:Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.09.012
更新日期：2008-01-15 00:00:00
abstract：:Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein associated with endothelial tight junctions (TJ). Based on a yeast two-hybrid screen, we have identified the membrane-associated guanylate kinase protein MAGI-1 as an intracellular binding partner of ESAM. MAGI-1 is a m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.07.010
更新日期：2004-10-15 00:00:00
abstract：:Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-u...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4804
更新日期：2000-03-15 00:00:00
abstract：:hnRNP I, also referred to as polypyrimidine tract binding protein, is one of the proteins associated with nascent pre-mRNA in the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As for all karyophilic proteins, the nuclear import of hnRNP proteins requires specific sequence determinants that in many instanc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3677
更新日期：1997-08-25 00:00:00
abstract：:We have been working with a recently immortalized Sertoli cell line, SF7, that appears to produce sleeves, or hollow tubules, when cultivated on a layer of growth factor-reduced Matrigel (GFR-Matrigel) in medium supplemented with serum. We tried to determine which components of GFR-Matrigel and serum provide the envir...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4630
更新日期：1999-10-10 00:00:00
abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.04.021
更新日期：2009-07-15 00:00:00
abstract：:We evaluated cellular mechanisms involved in the activation pathway of matrix prometalloproteinase-2 (pro-MMP-2), an enzyme implicated in the malignant progression of many tumor types. Membrane type-1 matrix metalloproteinase (MT1-MMP) cleaves the N-terminal prodomain of pro-MMP-2 thus generating the activation interm...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5118
更新日期：2001-02-15 00:00:00
abstract：:Laminin is a major component of the basal lamina upon which all epithelial cells reside in vivo. The synthesis of basal lamina components and their subsequent assembly into a morphologically distinct basal lamina is a differentiated function of epithelial cells in vivo. Ultrastructural studies in our laboratory show t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90036-8
更新日期：1992-11-01 00:00:00
abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90288-7
更新日期：1989-05-01 00:00:00
abstract：:We have used Xenopus egg extract to investigate the requirements for reactivation of DNA replication in nuclei isolated from terminally differentiated chicken erythrocytes. Previous work has shown that reactivation of erythrocyte nuclei in egg extract is accompanied by chromatin decondensation, nuclear envelope reform...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3520
更新日期：1997-05-01 00:00:00
abstract：:Utilizing both the TET-OFF and TET-ON systems in combination with transcriptional control elements of the Tie-2 gene, we have established a series of transgenic activator and responder mice for TET-regulated endothelial cell-specific transgene expression in double transgenic mouse embryos and in adult mice. TET-regula...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.026
更新日期：2008-04-01 00:00:00
abstract：:Non-small cell lung carcinoma (NSCLC) is characterized by resistance to drug-induced apoptosis, which might explain the survival of lung cancer cells following treatment. Recently we have shown that the broad-range kinase inhibitor staurosporine (STS) reactivates the apoptotic machinery in U1810 NSCLC cells [Joseph et...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.12.017
更新日期：2005-04-15 00:00:00
abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3470
更新日期：1997-03-15 00:00:00
abstract：:The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.017
更新日期：2008-04-01 00:00:00
abstract：:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3811
更新日期：1997-12-15 00:00:00
