An in vitro tubule assay identifies HGF as a morphogen for the formation of seminiferous tubules in the postnatal mouse testis.

abstract：:Most cases of familial early-onset Alzheimer's disease are caused by mutations in the presenilin 1 (PS1) gene. However, the cellular functions of PS1 are unknown. We showed predominant localization of PS1 to cell-cell contacts of the plasma membrane in human prostate epithelial tissue and in a human epithelial cell li...

journal_title：Experimental cell research

authors： Singh N,Talalayeva Y,Tsiper M,Romanov V,Dranovsky A,Colflesh D,Rudamen G,Vitek MP,Shen J,Yang X,Goldgaber D,Schwarzman AL

更新日期：2001-02-01 00:00:00

abstract：:The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...

journal_title：Experimental cell research

authors： Coppock DL,Buffolino P,Kopman C,Nathanson L

更新日期：1995-11-01 00:00:00

abstract：:LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specifi...

journal_title：Experimental cell research

authors： Mils V,Lee SM,Joly W,Hang EW,Baldin V,Waye MM,Ducommun B,Tsui SK

更新日期：2003-04-15 00:00:00

abstract：:MyD88, a protein implicated in interleukin-1 signaling, was localized in HeLa cells transiently transfected with an epitope-tagged (flag) version of MyD88. Overexpression of MyD88 can induce apoptosis. We have analyzed the fine structural intracellular distribution of MyD88 using immunoelectron microscopy. MyD88 is lo...

journal_title：Experimental cell research

authors： Jaunin F,Burns K,Tschopp J,Martin TE,Fakan S

更新日期：1998-08-25 00:00:00

abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

journal_title：Experimental cell research

authors： Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

更新日期：2004-08-15 00:00:00

abstract：BACKGROUND:Müller cell gliosis not only plays an important physiological role by maintaining retinal neuronal homeostasis but is also associated with multiple pathological events in the retina, including optic nerve crush (ONC) injury. Modulating Müller cell gliosis contributes to the creation of a permissive environme...

journal_title：Experimental cell research

authors： Li HJ,Sun ZL,Pan YB,Sun YY,Xu MH,Feng DF

更新日期：2019-02-15 00:00:00

abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

journal_title：Experimental cell research

authors： Moore SA

更新日期：1987-08-01 00:00:00

abstract：:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

journal_title：Experimental cell research

authors： Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

更新日期：1996-08-25 00:00:00

abstract：:Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

journal_title：Experimental cell research

authors： deHart GW,Healy KE,Jones JC

更新日期：2003-02-01 00:00:00

abstract：:Nuclear-envelope nucleoside triphosphatase activity (NTPase), an enzymatic activity thought to participate in RNA transport, was localized in rat liver in situ after brief perfusion with 3% paraformaldehyde. Reaction product was distributed along the nucleoplasmic side of the nuclear envelope (NE) in heterochromatin, ...

journal_title：Experimental cell research

authors： Clawson GA,Friend DS,Smuckler EA

更新日期：1984-11-01 00:00:00

abstract：:Pancreatic cancer is characterized by aggressive local invasion and early metastasis formation. Active migration of the pancreatic cancer cells is essential for these processes. We have shown previously that the pancreatic cancer cells lines CFPAC1 and IMIM-PC2 show high migratory activity, and we have investigated he...

journal_title：Experimental cell research

authors： Stock AM,Hahn SA,Troost G,Niggemann B,Zänker KS,Entschladen F

更新日期：2014-08-15 00:00:00

abstract：:Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...

journal_title：Experimental cell research

authors： Kuang E,Wan Q,Li X,Xu H,Zou T,Qi Y

更新日期：2006-07-01 00:00:00

abstract：:The development of Sjögren's syndrome (SS) is accompanied by B cell hyperproliferation and mutation. Our previous study identified aberrant expression of BST-2 (also known as Tetherin/CD317) in B cells from either the peripheral blood or infiltrated salivary glands. However, the roles of BST-2 in the regulation of B c...

journal_title：Experimental cell research

authors： Fu J,Shi H,Zhan T,Li H,Ye L,Xie L,Wang Z,Wang B,Zheng L

更新日期：2021-01-01 00:00:00

abstract：:Retinol binding protein (RBP) is the plasma transport protein of retinol. Mobilization of RBP from the liver stores is stimulated by retinol. During vitamin A deficiency, RBP secretion is specifically inhibited while its rate of biosynthesis is unaffected. As a consequence, RBP, as apoprotein, accumulates inside the e...

journal_title：Experimental cell research

authors： Tosetti F,Ferrari N,Pfeffer U,Brigati C,Vidali G

更新日期：1992-06-01 00:00:00

abstract：:The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

journal_title：Experimental cell research

authors： Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

更新日期：2019-10-15 00:00:00

abstract：:Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase...

journal_title：Experimental cell research

authors： Browne CL,Bower WA,Palazzo RE,Rebhun LI

更新日期：1990-05-01 00:00:00

abstract：:A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times....

journal_title：Experimental cell research

authors： Mather IH,Jarasch ED,Bruder G,Heid HW,Mepham TB

更新日期：1984-03-01 00:00:00

abstract：:Tumor hypoxia correlates to aggressive disease, and while this is explained by a variety of factors, one clue to understand this phenomena was the finding that hypoxia induces a de-differentiated, stem cell-like phenotype in neuroblastoma and breast tumor cells. The hypoxia inducible transcription factors (HIFs) are r...

journal_title：Experimental cell research

authors： Mohlin S,Wigerup C,Jögi A,Påhlman S

更新日期：2017-07-15 00:00:00

abstract：:The germinal vesicle (GV) of Xenopus laevis is an enormous nucleus that contains 18 giant lampbrush chromosomes and thousands of inclusions. The inclusions are primarily of three types: approximately 1500 extrachromosomal nucleoli, 50-100 Cajal bodies, and several thousand B-snurposomes, which correspond to speckles o...

journal_title：Experimental cell research

authors： Gall JG,Wu Z,Murphy C,Gao H

更新日期：2004-05-15 00:00:00

abstract：:p107 and p130 were originally identified as targets of the transforming domains of viral oncoproteins encoded by small DNA tumor viruses. Together with pRB, the protein product of the retinoblastoma gene (Rb), p107 and p130 represent a family of closely related proteins that play critical roles in the regulation of ce...

journal_title：Experimental cell research

authors： Classon M,Dyson N

更新日期：2001-03-10 00:00:00

abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...

journal_title：Experimental cell research

authors： Huang Y,Wang JJ,Ghosh S,Liu JL

更新日期：2017-05-15 00:00:00

abstract：:Taking the advantage of single-molecule imaging, our recent study has revealed surprisingly long processive movement of a Formin protein, mDia1, surfing along with the growing end of actin filaments in living cells. This finding provides direct evidence for the ability of Formins to function as processive cappers that...

journal_title：Experimental cell research

authors： Watanabe N,Higashida C

更新日期：2004-11-15 00:00:00

abstract：:The epidermal growth factor (EGF) receptor is well known as a mediator of mitogenic signaling and its tyrosine kinase activity has been suggested as a viable target in cancer chemotherapy. To explore the consequences of abolishing the kinase activity of this receptor, we have utilized a potent and specific inhibitor o...

journal_title：Experimental cell research

authors： Nelson JM,Fry DW

更新日期：1997-06-15 00:00:00

abstract：:The protein kinase mammalian target of rapamycin (mTOR) is a central regulator of cell proliferation and growth, with the ribosomal subunit S6 kinase 1 (S6K1) as one of the key downstream signaling effectors. A critical role of mTOR signaling in skeletal muscle differentiation has been identified recently, and an unus...

journal_title：Experimental cell research

authors： Park IH,Erbay E,Nuzzi P,Chen J

更新日期：2005-09-10 00:00:00

abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...

journal_title：Experimental cell research

authors： Lv N,Kim EK,Song MY,Choi HN,Moon WS,Park SJ,Park JW,Kwon KB,Park BH

更新日期：2009-07-15 00:00:00

abstract：:Bovine papilloma virus-transformed hamster embryo fibroblasts (HEF-BPV) reacted to exposure to tricyclodecan-9-yl-xanthogenate (D609) with immediate reversion to the growth kinetics and the flat morphology of the untransformed parental cells. After six population doublings in the presence of D609, clones which display...

journal_title：Experimental cell research

authors： Amtmann E,Müller K,Knapp A,Sauer G

更新日期：1985-12-01 00:00:00

abstract：:The movement of a cell through the sequential phases of apoptosis is accompanied by a progressive decrease in cell size with loss in protein mass. In lymphocytes from Hiv-infected persons, protein loss during apoptosis is due to increased protein degradation rather than decreased synthesis. To identify and characteriz...

journal_title：Experimental cell research

authors： Piedimonte G,Crinelli R,Della Salda L,Corsi D,Pennisi MG,Kramer L,Casabianca A,Sarli G,Bendinelli M,Marcato PS,Magnani M

更新日期：1999-05-01 00:00:00

abstract：:Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtration of a crude homoge...

journal_title：Experimental cell research

authors： Rosenman SJ,Fenderson BA,Hakomori SI

更新日期：1989-02-01 00:00:00

abstract：:Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

journal_title：Experimental cell research

authors： Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

更新日期：1998-07-10 00:00:00

abstract：:We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...

journal_title：Experimental cell research

authors： Zugaza JL,Casabiell XA,Bokser L,Eiras A,Beiras A,Casanueva FF

更新日期：1995-07-01 00:00:00