Abstract:
:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracellular matrix, in proliferative responses, and in the expression of critical proteins. The present study utilizes collagen gel contraction assays and the analysis of integrin receptor subunit expression to evaluate cell-matrix interactions. In the presence of serum and transforming growth factor-beta 1 (TGF) or TGF-beta 1 alone, VSMC isolated from the abdominal aorta (AA-VSMC) were found to contract collagen matrices to a significantly greater extent than VSMC from the thoracic aorta (TA-VSMC). However, in TA-VSMC, beta 1 integrin and gel contraction were stimulated only in the presence of serum factors. Metabolic labeling and immunoprecipitation of integrin subunits revealed that TGF-beta 1 induced beta 1 and alpha 5 integrin subunits in AA-VSMC four-and ninefold, respectively. AA-VSMC gel contraction stimulated by serum and TGF-beta 1 alone was inhibited with anti-beta 1 integrin antibody by 70 and 100%, respectively. However, the beta 1 integrin-specific antibody inhibited serum-induced TA-VSMC gel contraction by 25%. The data suggest that vascular smooth muscle cell ontogeny is an important determinant of cell function, phenotype, and response to growth factors such as TGF-beta 1.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist THdoi
10.1006/excr.1996.0258subject
Has Abstractpub_date
1996-08-25 00:00:00pages
135-45issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(96)90258-Xjournal_volume
227pub_type
杂志文章abstract::The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90459-l
更新日期:1992-03-01 00:00:00
abstract::The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90351-t
更新日期:1991-06-01 00:00:00
abstract::We have compared dye coupling in pairs of small (less than 10 microns in diameter) and large (greater than 20 microns in diameter) keratinocytes isolated from normal human epidermis, using Lucifer yellow microinjection. Under control conditions, dye coupling was found in only 1 out of the 25 small pairs tested, wherea...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90294-i
更新日期:1992-08-01 00:00:00
abstract::Tendinopathy is a common and disabling condition that is difficult to treat. The pathomolecular events behind tendinopathy remain uncertain. Micro-RNAs (miRNAs, miRs) are short non-coding RNAs that regulate gene expression and may play a role in tendinopathy development. Tenocytes were obtained from human patellar ten...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.03.008
更新日期:2019-05-15 00:00:00
abstract::The possible role of transferrin receptors in the diacytosis of human asialotransferrin type 3 (HAsTf-3) by the rat liver was studied in vivo. A trace dose of the ligand was allowed to compete for hepatic binding sites against diferric transferrin, the concentration of which was varied between 5 400- and 18 000-fold. ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90230-7
更新日期:1985-09-01 00:00:00
abstract::The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4586
更新日期:1999-09-15 00:00:00
abstract::The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-recepto...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(05)80074-6
更新日期:1992-05-01 00:00:00
abstract::We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1115
更新日期:1994-05-01 00:00:00
abstract::Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.09.012
更新日期:2008-01-15 00:00:00
abstract::Glycosaminoglycans (heparins, dermatan sulfate, chondroitin sulfate) with different structures and physicochemical properties were evaluated for their capacity to influence proliferation and differentiation of U-937 cell line. The contrasting and specific effects of glycosaminoglycans (depending on their structures an...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1323
更新日期:1994-11-01 00:00:00
abstract::In confluent keratinocyte monolayers, desmosomal adhesion gradually becomes calcium-independent and this is associated with an increase in the strength of intercellular adhesion (hyper-adhesion). In this study, we investigated the functional and molecular significance of hyper-adhesion in a system challenged by autoim...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.10.005
更新日期:2010-02-15 00:00:00
abstract::We investigated whether cell-permeable, synthetic ceramide (C6 ceramide) could induce apoptosis in Fas-resistant Hodgkin's disease (HD)-derived cell lines. Despite strongly expressing the Fas-receptor, two of three HD-derived cell lines were resistant to Fas-mediated apoptosis. This resistance to Fas could not be attr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4773
更新日期:2000-02-25 00:00:00
abstract::Pure melanophore populations isolated from the tail skin of the tadpole, Rana catesbeiana, were mass cultured for a period of 2-3 years. All cell lines of amphibian melanophores studied exhibited growth crisis (in vitro ageing) followed by spontaneous transformation to a continuous cell line, as shown by changes in gr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90396-8
更新日期:1983-11-01 00:00:00
abstract::The cancer cells residing in the hypoxic layer are resistant to radiation and these are ones responsible for cancer recurrence after radiation therapy. One of the reasons why hypoxic cancer cells acquire radioresistance may be attributable to changes in the gene expression profile by the activation of hypoxia inducibl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.03.009
更新日期:2015-05-01 00:00:00
abstract::Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5408
更新日期:2002-01-15 00:00:00
abstract::Hypoxia/reoxygenation (H/R) injury is characterized by microfilament reorganization in cardiomyocytes. Previous studies have shown that myofibrillogenesis regulator-1 (MR-1) is expressed in the myocardium and promotes actin organization in cardiomyocytes. The purpose of this study was to investigate the role of MR-1 i...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.05.026
更新日期:2015-10-01 00:00:00
abstract::KILLER/DR5 is a death-domain-containing proapoptotic receptor that binds to the cytotoxic ligand TRAIL. It was originally reported that induction of KILLER/DR5 mRNA following DNA damage was p53-dependent, but some drugs that induce apoptosis can upregulate KILLER/DR5 mRNA expression in cell lines with mutated p53. We ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5073
更新日期:2001-01-15 00:00:00
abstract::A combination of broadly expressed transcriptional activators and spatially restricted repressors are used to pattern embryos into cells of different fate. Transcriptional co-regulators are essential mediators of transcription factor function, and contribute to selective transcriptional responses in embryo development...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2013.10.010
更新日期:2014-02-01 00:00:00
abstract::A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90604-9
更新日期:1984-08-01 00:00:00
abstract::Insulin mimetics, including zinc containing compounds, have previously been shown to influence chondrogenesis as it relates to healing of fractures in various preclinical models. However, the mechanism by which these compounds drive chondrogenic differentiation is yet undefined. Here, via next-generation sequencing (N...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.112436
更新日期:2021-01-21 00:00:00
abstract::As chemical reactions related to the regulation of cell proliferation are governed by availability, amount, and concentration of relevant molecules, it has been suggested that cell size is an important factor in the control of cell cycle. We have measured the size of proliferating cells of Allium cepa roots in which g...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90056-6
更新日期:1989-11-01 00:00:00
abstract::The subcellular distribution of tissue transglutaminase in human umbilical vein endothelial cells and human arterial and venous smooth muscle cells was examined. Double-immunofluorescence staining of smooth muscle cells and endothelial cells with anti-transglutaminase antisera and rhodamine-tagged phalloidin revealed ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3448
更新日期:1997-02-25 00:00:00
abstract::This study was designed to elucidate the mechanisms involved in elevated cell death arising from an altered endogenous oxidant state. Increased levels of cell death were detected in cells lacking Gpx1 following the addition of exogenous H2O2. This increased apoptosis correlated with a down-regulation in the activation...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.07.018
更新日期:2004-11-01 00:00:00
abstract::Repeated injury to the stratum corneum of mammalian skin (caused by friction, soaps, or organic solvents) elicits hyperkeratosis and epidermal thickening. Functionally, these changes serve to restore the cutaneous barrier and protect the organism. To better understand the molecular and cellular basis of this response,...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.021
更新日期:2007-08-15 00:00:00
abstract::The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90709-2
更新日期:1984-01-01 00:00:00
abstract::A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90190-x
更新日期:1987-04-01 00:00:00
abstract::Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.02.009
更新日期:2005-05-15 00:00:00
abstract:BACKGROUND:Emerging studies demonstrate that long noncoding RNAs (lncRNAs) play crucial roles in hepatocarcinogenesis through various mechanisms. LncRNA CCAT2 was a newly discovered lncRNA and amplified in several cancers. However, the mechanisms involved in function of CCAT2 in hepatocellular carcinoma (HCC) remain to...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.03.029
更新日期:2019-06-01 00:00:00
abstract::Specific mitochondrial incorporation of 10 N-nonyl acridine orange (NAO) is demonstrated by subcellular fractionation of rat hepatocytes. Moreover, comparative studies with NAO and rhodamine 123 (Rh 123) prove that acridine orange-derivative uptake is independent of transmembrane mitochondrial potential, a property al...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90219-z
更新日期:1990-01-01 00:00:00
abstract::Autophagy has been reported to have a pivotal role in maintaining stemness, regulating immunomodulation and enhancing the survival of mesenchymal stem cells (MSCs). However, the effect of autophagy on MSC metabolism is largely unknown. Here, we report a workflow for examining the impact of autophagy on human placenta-...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.09.009
更新日期:2018-11-01 00:00:00