Abstract:
:The movement of a cell through the sequential phases of apoptosis is accompanied by a progressive decrease in cell size with loss in protein mass. In lymphocytes from Hiv-infected persons, protein loss during apoptosis is due to increased protein degradation rather than decreased synthesis. To identify and characterize the proteolytic enzymes or enzyme systems involved in this process, we studied several features of protein turnover in lymphocytes from peripheral blood and lymph nodes during the natural and experimental infection by feline immunodeficiency virus (Fiv). This animal model allowed us to integrate in vivo results with in vitro observations of protein damage. Here we report that protein breakdown in apoptotic cells is concomitant with the activation of the ATP and ubiquitin-dependent multicatalytic system (proteasome). We suggest that proteasome activation is part of the proteolytic cascade in the execution phases of apoptosis in AIDS.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Piedimonte G,Crinelli R,Della Salda L,Corsi D,Pennisi MG,Kramer L,Casabianca A,Sarli G,Bendinelli M,Marcato PS,Magnani Mdoi
10.1006/excr.1999.4410keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
381-90issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(99)94410-5journal_volume
248pub_type
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