Abstract：
:Most cases of familial early-onset Alzheimer's disease are caused by mutations in the presenilin 1 (PS1) gene. However, the cellular functions of PS1 are unknown. We showed predominant localization of PS1 to cell-cell contacts of the plasma membrane in human prostate epithelial tissue and in a human epithelial cell line HEp2 stably transfected with an inducible PS1 construct. PS1 co-immunoprecipitated with beta-catenin from cell lysates of stable transfectants. Conversely, PS1 lacking the PS1-beta-catenin interaction site did not co-immunoprecipitate with beta-catenin and was not recruited to the cell-cell contacts. L cells, which do not form tight intercellular contacts, formed clusters of adhered cells after stable transfection with GFP-PS1 cDNA and demonstrated a clear preference for independent aggregation in the mixed cultures. However, L cells transfected with mutant GFP-PS1 constructs, which had a truncated N-terminus of PS1 or deleted PS1-beta-catenin interaction site, failed to form intercellular contacts. In addition, in primary cultures of mouse cortical neurons PS1 was highly concentrated on the surface of extended growth cones. Taken together, our results suggest an important role of PS1 in intercellular adhesion in epithelial cells and neurons.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Singh N,Talalayeva Y,Tsiper M,Romanov V,Dranovsky A,Colflesh D,Rudamen G,Vitek MP,Shen J,Yang X,Goldgaber D,Schwarzman ALdoi
10.1006/excr.2000.5098keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
1-13issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(00)95098-5journal_volume
263pub_type
杂志文章abstract：:We report the isolation of six cell lines (designated EB cell lines) from cultures of the hypoxanthine guanine phosphoribosyl transferase-deficient (HGPRT-) feeder-dependent embryonal carcinoma cell line PSA4TG12 which have undergone in vitro differentiation, and of clonal derivatives of these lines. Whereas some line...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90167-2
更新日期：1983-10-15 00:00:00
abstract：:We had identified earlier a germ cell-specific lamin of 60 kDa in rat which is related to somatic lamin B. This polypeptide was shown to be the only major component organizing the lamina structure of round spermatids. In the present study, we find that this 60-kDa polypeptide persists in the testicular and epididymal ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90151-w
更新日期：1992-01-01 00:00:00
abstract：:In the fungus Achlya, sexual development in the male strain E87 is mediated by the steroid hormone antheridiol. Treatment of vegetatively growing cells of E87 with antheridiol resulted in changes in the [35S]methionine labeling of several secreted proteins. The most heavily labeled group of proteins observed in the se...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90555-0
更新日期：1986-03-01 00:00:00
abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.02.001
更新日期：2015-04-10 00:00:00
abstract：:Recently, SIRT5 was reported to be a predominant desuccinylase and demalonylase in mitochondria. Ablation of SIRT5 enhances the systemic succinylation and malonylation of mitochondrial proteins, including various metabolic enzymes; however, its function in pancreatic β cells has not yet been clarified. In this study, ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.08.011
更新日期：2018-10-01 00:00:00
abstract：:When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90788-2
更新日期：1984-11-01 00:00:00
abstract：:Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.027
更新日期：2013-03-10 00:00:00
abstract：:Tendinopathy is a common and disabling condition that is difficult to treat. The pathomolecular events behind tendinopathy remain uncertain. Micro-RNAs (miRNAs, miRs) are short non-coding RNAs that regulate gene expression and may play a role in tendinopathy development. Tenocytes were obtained from human patellar ten...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.03.008
更新日期：2019-05-15 00:00:00
abstract：:A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90578-1
更新日期：1986-03-01 00:00:00
abstract：:Transforming growth factor beta regulates many biological processes including cell motility and invasion. Podosomes are specialized F-actin rich structures found in normal cells, such as osteoclasts and macrophages. Tumor cells often form related structures called invadopodia that are thought to promote invasion and m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.09.013
更新日期：2008-11-15 00:00:00
abstract：:The effect of RU 486, a synthetic steroid that is a powerful antagonist of glucocorticoid hormones, was tested on the transcription of several glucocorticoid-regulated genes in different cell types: inflammatory murine macrophages and two human mammary gland-derived cell lines, MDA-MB-231 and HBL-100. The transcriptio...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90282-5
更新日期：1987-12-01 00:00:00
abstract：:A ts mutant clone, tsJT60, was isolated from Fisher rat cell line, 3Y1. During the exponential growth at both 34 and 39.5 degrees C, tsJT60 did not appear as ts mutant cells. However, once entered resting state (G0) under serum deprivation at the confluent state, they could re-enter S phase at 34 degrees C but could n...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90701-8
更新日期：1984-01-01 00:00:00
abstract：:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3878
更新日期：1998-03-15 00:00:00
abstract：:In order to test the existence of mechanical coupling between the rotational movements of two adjacent nuclei, we prepared binucleate 3T3 cells and observed their nuclear movements by near infrared microscopy and recorded them with time-lapse video techniques. We found that 49 out of 110 (44%) of the selected binuclea...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90512-4
更新日期：1986-09-01 00:00:00
abstract：:Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.09.026
更新日期：2007-01-01 00:00:00
abstract：:It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.04.002
更新日期：2006-08-01 00:00:00
abstract：:Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：:A combination of broadly expressed transcriptional activators and spatially restricted repressors are used to pattern embryos into cells of different fate. Transcriptional co-regulators are essential mediators of transcription factor function, and contribute to selective transcriptional responses in embryo development...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2013.10.010
更新日期：2014-02-01 00:00:00
abstract：:An in vitro system has been employed to study the apoptotic mechanisms in the AK-5 tumor which is a spontaneously regressing rat histiocytoma. Cytosolic extracts of tumor cells primed for apoptosis using dexamethasone and immune serum from tumor-regressing animals were able to induce apoptosis in intact nuclei and rep...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3730
更新日期：1997-11-01 00:00:00
abstract：:Transforming growth factor beta (TGFbeta) can modulate the activity of various MAP kinases. However, how this pathway may mediate TGFbeta-induced malignant phenotypes remains elusive. We investigated the role of autocrine TGFbeta signaling through MAP kinases in the regulation of cell survival in breast carcinoma MCF-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.016
更新日期：2007-05-01 00:00:00
abstract：:A method for coating liposomes with transferrin is described. Such liposomes have been used for carrying exogenous DNA to rabbit bone marrow (BM) precursor cells, in vivo. A large amount of the DNA contained in the liposomes was delivered specifically into the erythroblasts of the test animals. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90056-x
更新日期：1986-06-01 00:00:00
abstract：:Stress-activated protein (SAP) kinases and the mitochondrial pro-apoptotic Bcl-2 protein Bak are important regulators of apoptosis. Reduced expression of Bak increases cellular resistance to the anticancer agent cisplatin, and we report here that mouse embryo fibroblasts deficient in the SAP kinase jnk1 are highly res...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.01.023
更新日期：2006-05-15 00:00:00
abstract：:Liver cells of new-born rats, which were found to be able to form spheroidal aggregates when cultured on a nonadherent plastic substratum, were studied under various conditions of culture, mainly by adding different nutrients and growth factors to the culture medium. Analysis of hepatocyte-specific functions was carri...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90260-h
更新日期：1990-07-01 00:00:00
abstract：:The rheumatic diseases are characterized by the production of autoantibodies that are usually directed against components of the cell nucleus. In this communication, we describe autoantibodies that recognize DNA topoisomerase II (anti-topoII) present in the serum of a patient with systemic lupus erythematosus. Several...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90067-0
更新日期：1989-02-01 00:00:00
abstract：:Huntingtin interacting protein HYPK (Huntingtin Yeast Partner K) is an intrinsically unstructured protein having chaperone-like activity and can suppress mutant huntingtin aggregates and toxicity in cell model of Huntington's Disease (HD). Heat shock response is an adaptive mechanism of cells characterized by upregula...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.03.021
更新日期：2016-05-01 00:00:00
abstract：:The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90557-9
更新日期：1985-02-01 00:00:00
abstract：:We previously showed that the phenotypes of adipocyte progenitors and macrophages were close. Using functional analyses and microarray technology, we first tested whether this intriguing relationship was specific to adipocyte progenitors or could be shared with other progenitors. Measurements of phagocytic activity an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.06.034
更新日期：2006-10-15 00:00:00
abstract：:The effect of the physiological vitamin D metabolite 24R, 25-dihydroxyvitamin D3 [24R,25(OH)2D3] on human osteoblastic cells was assessed. Physiological concentrations (10(-9)-10(-8) M) of 24R, 25(OH)2D3 significantly increased the cyclic guanosine 5'-monophosphate (cGMP) content in the human osteoblastic cells by app...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4189
更新日期：1998-10-10 00:00:00
abstract：:Like most epithelial cells that are isolated from tissues and placed in culture, the phenotype of human retinal pigment epithelial cells (RPE) in vitro is more variable than for the same cells in situ. The phenotypic heterogeneity of the cultures has been attributed to varying stages of differentiation of the cells in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1176
更新日期：1994-07-01 00:00:00
abstract：:p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.007
更新日期：2006-10-15 00:00:00
