Abstract：
:A combination of broadly expressed transcriptional activators and spatially restricted repressors are used to pattern embryos into cells of different fate. Transcriptional co-regulators are essential mediators of transcription factor function, and contribute to selective transcriptional responses in embryo development. A two step mechanism of transcriptional regulation is discussed, where remodeling of chromatin is initially required, followed by stimulation of recruitment or release of RNA polymerase from the promoter. Transcriptional co-regulators are essential for both of these steps. In particular, most co-activators are associated with histone acetylation and co-repressors with histone deacetylation. In the early Drosophila embryo, genome-wide studies have shown that the CBP co-activator has a preference for associating with some transcription factors and regulatory regions. The Groucho, CtBP, Ebi, Atrophin and Brakeless co-repressors are selectively used to limit zygotic gene expression. New findings are summarized which show that different co-repressors are often utilized by a single repressor, that the context in which a co-repressor is recruited to DNA can affect its activity, and that co-regulators may switch from co-repressors to co-activators and vice versa. The possibility that co-regulator activity is regulated and plays an instructive role in development is discussed as well. This review highlights how findings in Drosophila embryos have contributed to the understanding of transcriptional regulation in eukaryotes as well as to mechanisms of animal embryo patterning.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Mannervik Mdoi
10.1016/j.yexcr.2013.10.010subject
Has Abstractpub_date
2014-02-01 00:00:00pages
47-57issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(13)00439-4journal_volume
321pub_type
杂志文章,评审abstract：:The correct functioning of epidermal growth factor receptor (EGFR), a receptor tyrosine kinase, is required for normal skin development and homeostasis. Cellular hyperproliferation induced by dysregulation of EGFR is tightly associated with structural and functional defects of hair follicles, skin lesions, and tumorig...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111648
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abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.09.020
更新日期：2006-01-01 00:00:00
abstract：:Barrier-to-autointegration factor (BAF) is an essential chromatin protein conserved in metazoans. BAF has roles in nuclear assembly, chromatin organization, gene expression, and gonad development and is exploited by retroviruses. BAF forms stable dimers that bind nonspecifically to dsDNA and specifically to LEM-domain...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2005.11.013
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abstract：:A monoclonal antibody, G8, which recognizes a form of tubulin (G8-tubulin) with a novel distribution in Rat-1 cells and Potorous tridactylis kidney (Ptk-2) cells was isolated. G8 labeled the interphase cytoskeleton of Rat-1 fibroblasts but not mitotic spindles or midbodies. G8 also stained a fiber network in some but ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90345-5
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.035
更新日期：2005-04-01 00:00:00
abstract：:As chemical reactions related to the regulation of cell proliferation are governed by availability, amount, and concentration of relevant molecules, it has been suggested that cell size is an important factor in the control of cell cycle. We have measured the size of proliferating cells of Allium cepa roots in which g...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90056-6
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journal_title：Experimental cell research
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更新日期：2018-03-01 00:00:00
abstract：:Macrophages are resistant to cell death and are one of HIV reservoirs. HIV viral protein Vpr has the potential to promote infection of and survival of macrophages, which could be a highly significant factor in the development and/or maintenance of macrophage viral reservoirs. However, the impact of vpr on macrophages ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.12.024
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abstract：:The extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2016-04-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1990-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.11.017
更新日期：2004-03-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.06.012
更新日期：2019-09-01 00:00:00
abstract：:The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.10.022
更新日期：2004-03-10 00:00:00
abstract：:Pseudolaric acid B (PAB) is the major bioactive constituent in the root bark of Pseudolarix kaempferi and has been reported to have cytotoxicity against tumor cells. Our in vivo experiments showed that PAB could inhibit gastric cancer cell lung metastasis in a nude mouse haematogenous dissemination model. To evaluate ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.01.012
更新日期：2017-03-01 00:00:00
abstract：:Dynamic subcellular localization is an important regulatory mechanism for many proteins. cIAP1 and cIAP2 are two closely related members of inhibitor of apoptosis (IAP) family that play a role both as caspase inhibitors and as mediators of tumor necrosis factor (TNF) receptor signaling. Here, we report that cIAP1 and ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.04.040
更新日期：2004-08-15 00:00:00
abstract：:The CDC25Mm gene codes for Ras-guanine nucleotide exchange factors. Four different full-length cDNA clones derived from the same gene and coding for proteins of different sizes that have in common the last 661 amino acids have been isolated from mouse brain. In order to investigate the expression of the products of th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1048
更新日期：1994-02-01 00:00:00
abstract：:Skeletal muscles cope with a large range of activities, from being able to support the body weight during long periods of upright standing to perform explosive movements in response to an unexpected threat. This requires systems for energy metabolism that can provide energy during long periods of moderately increased ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2010-11-01 00:00:00
abstract：:Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenoviru...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5352
更新日期：2001-11-01 00:00:00
abstract：:Among the cellular events that are associated with the process of endochondral ossification is an incremental increase in chondrocyte basal intracellular free Ca(2+) concentration ([Ca(2+)](i)) from 50 to 100 nM. To determine if this rise in [Ca(2+)](i) functionally participates in the maturational process of growth p...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5527
更新日期：2002-06-10 00:00:00
abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90431-8
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2006-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2007-06-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90472-7
更新日期：1988-08-01 00:00:00
abstract：:Endocytosis of signaling receptors, such as epidermal growth factor receptor (EGFR), tightly controls the signal transduction process triggered by ligand activation of these receptors. To identify new regulators of the endocytic trafficking of EGFR an RNA interference screen was performed for genes involved in ubiquit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.04.017
更新日期：2010-08-01 00:00:00
abstract：:Electrospinning permits fabrication of biodegradable matrices that can resemble the both scale and mechanical behavior of the native extracellular matrix. However, achieving high-cellular density and infiltration of cells within matrices with traditional technique remain challenging and time consuming. The cell electr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.08.007
更新日期：2015-11-01 00:00:00
abstract：:The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1262
更新日期：1993-10-01 00:00:00
abstract：:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.03.021
更新日期：2004-07-01 00:00:00
abstract：:Heat shock proteins (HSPs) have molecular chaperone functions in protein biogenesis as well as cytoprotective functions against deleterious environmental stresses, and they work mainly inside of the cells. HSPs are usually induced in living cells that have been exposed to mild stresses or have recovered from severe st...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.07.014
更新日期：2005-10-15 00:00:00
