Enhanced expression of heat shock proteins in gradually dying cells and their release from necrotically dead cells.

abstract：:The growth rate of malignant F9 embryonal carcinoma cells slows considerably following all-trans-retinoic acid-induced differentiation into benign parietal endoderm. To determine the mechanism of this process, we examined the expression of cyclins D1, D2, and D3 and the activity of their associated kinases. Cyclin D1 ...

journal_title：Experimental cell research

authors： Li Y,Glozak MA,Smith SM,Rogers MB

更新日期：1999-12-15 00:00:00

abstract：:We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...

journal_title：Experimental cell research

authors： Ji Q,Huang CH,Peng J,Hashmi S,Ye T,Chen Y

更新日期：2007-04-15 00:00:00

abstract：:The NADH-linked substrates pyruvate, L-glutamine, and beta-hydroxybutyrate, while enhancing the rate of oxygen consumption, also increased the formation of DNA single-strand breaks induced by tert-butylhydroperoxide in intact U937 cells. A cause-effect relationship between these two parameters was established by showi...

journal_title：Experimental cell research

authors： Guidarelli A,Brambilla L,Clementi E,Sciorati C,Cantoni O

更新日期：1997-11-25 00:00:00

abstract：:The continuously decreasing willingness for liver donation aggravates treatment of end-stage liver diseases requiring organ transplantation as the only curative strategy. Cell therapy approaches using human hepatocytes or stem cell-derived hepatocyte-like cells may be a therapeutic option out of this dilemma. ABCB5-po...

journal_title：Experimental cell research

authors： Tietze L,Winkler S,Hempel M,Kluth MA,Tappenbeck N,Ganss C,Dooley S,Christ B

更新日期：2018-08-15 00:00:00

abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

journal_title：Experimental cell research

authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

更新日期：2008-08-01 00:00:00

abstract：:Oxysterol-binding protein (OSBP)-related proteins (ORPs) are conserved lipid binding proteins found in organisms ranging from yeast to mammals. Recent findings have indicated that these proteins mainly localize to contact sites of 2 different membranous organelles. ORP6, a member of the ORP subfamily III, is one of th...

journal_title：Experimental cell research

authors： Mochizuki S,Miki H,Zhou R,Kido Y,Nishimura W,Kikuchi M,Noda Y

更新日期：2018-09-15 00:00:00

abstract：:Cellular senescence is a state of irreversible cell cycle arrest in which normal cells at the end of their lifespan fail to enter into DNA synthesis upon serum or growth factor stimulation. We examined whether proteins required for G1/S cell cycle progression were irreversibly down-regulated in senescent human fibrobl...

journal_title：Experimental cell research

authors： Afshari CA,Vojta PJ,Annab LA,Futreal PA,Willard TB,Barrett JC

更新日期：1993-12-01 00:00:00

abstract：:Glycosaminoglycans (heparins, dermatan sulfate, chondroitin sulfate) with different structures and physicochemical properties were evaluated for their capacity to influence proliferation and differentiation of U-937 cell line. The contrasting and specific effects of glycosaminoglycans (depending on their structures an...

journal_title：Experimental cell research

authors： Volpi N,Petrini M,Conte A,Valentini P,Venturelli T,Bolognani L,Ronca G

更新日期：1994-11-01 00:00:00

abstract：:Human diploid fibroblasts (HDF) rarely, if ever, undergo spontaneous transformation to an immortalized cell type. Here we report the immortalization of an HDF cell line following transduction with cyclin A2 or cdk1 human genes via retroviral vectors. Fluorescence in situ hybridization (FISH) studies using the retrovir...

journal_title：Experimental cell research

authors： Luo P,Tresini M,Cristofalo V,Chen X,Saulewicz A,Gray MD,Banker DE,Klingelhutz AL,Ohtsubo M,Takihara Y,Norwood TH

更新日期：2004-04-01 00:00:00

abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...

journal_title：Experimental cell research

authors： Nakamura H,Morita T,Sato C

更新日期：1986-08-01 00:00:00

abstract：:Liver endothelium has been reported to possess membrane receptors for the iron-binding protein transferrin (Tf). Similarly, the core protein of proteoglycans (PG) associated with cell membrane in many cell systems can bind Tf. To find out if membrane-associated proteoglycans can explain Tf-binding ability of liver end...

journal_title：Experimental cell research

authors： Omoto E,Minguell JJ,Tavassoli M

更新日期：1990-03-01 00:00:00

abstract：:A heterogeneous RNP structure has been isolated from rat liver nuclei by a method previously used for the isolation of 30S RNP complexes carrying heterogeneous RNA (hnRNA) [1]. The RNP sediments in sucrose gradients with s-values of 70-110S. Formaldehyde-fixed preparations band at Q = 1.40 in isopycnic CsCl gradients....

journal_title：Experimental cell research

authors： Hatzoglou M,Adamtziki E,Margaritis L,Sekeris CE

更新日期：1985-03-01 00:00:00

abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

journal_title：Experimental cell research

authors： Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

更新日期：1998-05-25 00:00:00

abstract：:A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with ...

journal_title：Experimental cell research

authors： Haider SR,Juan G,Traganos F,Darzynkiewicz Z

更新日期：1997-08-01 00:00:00

abstract：:It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

journal_title：Experimental cell research

authors： Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

更新日期：2006-08-01 00:00:00

abstract：:We had identified earlier a germ cell-specific lamin of 60 kDa in rat which is related to somatic lamin B. This polypeptide was shown to be the only major component organizing the lamina structure of round spermatids. In the present study, we find that this 60-kDa polypeptide persists in the testicular and epididymal ...

journal_title：Experimental cell research

authors： Sudhakar L,Sivakumar N,Behal A,Rao MR

更新日期：1992-01-01 00:00:00

abstract：:Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 months. The morphological and cytochemical features of the two populations are quite similar to those of the orig...

journal_title：Experimental cell research

authors： Ferrero D,Gallo E,Lanfrancone L,Tarella C

更新日期：1983-08-01 00:00:00

abstract：:The formation of functional gap junctions between astrocytes was investigated during differentiation of these cells in culture. Precursor cells of GFA (glial fibrillary acidic) protein-positive astrocytes were cultured in a chemically defined medium as a homogeneous population. These cells were rarely coupled to one n...

journal_title：Experimental cell research

authors： Fischer G,Kettenmann H

更新日期：1985-08-01 00:00:00

abstract：:Cleavage of specific protein subsets is a key event in the execution of apoptosis. Protein degradation may serve for the structural alterations that result in cell self-destruction, but it may also function as a switch in the decisions between apoptosis and necrosis or apoptosis and cell proliferation. Here, we show t...

journal_title：Experimental cell research

authors： Schwab BL,Leist M,Knippers R,Nicotera P

更新日期：1998-02-01 00:00:00

abstract：:The aging proteostasis decline manifests in a failure of aging cells and organisms to properly respond to proteotoxic challenges. This proteostasis collapse has long been considered a hallmark of aging in nematodes, and has recently been shown to occur also in human cells upon entry to senescence, opening the way to e...

journal_title：Experimental cell research

authors： Meller A,Shalgi R

更新日期：2021-01-09 00:00:00

abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

journal_title：Experimental cell research

authors： List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

更新日期：2000-10-10 00:00:00

abstract：:In dog thyrocytes in primary culture, thyrotropin (TSH), acting through cyclic AMP, induces proliferation and differentiation expression, while tetradecanoylphorbol acetate (TPA) or epidermal growth factor (EGF) induces proliferation and dedifferentiation. In this work, we have investigated the regulation of mRNA expr...

journal_title：Experimental cell research

authors： Pirson I,Dumont JE

更新日期：1994-10-01 00:00:00

abstract：:A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...

journal_title：Experimental cell research

authors： Jiang WQ,Szekely L,Wendel-Hansen V,Ringertz N,Klein G,Rosén A

更新日期：1991-12-01 00:00:00

abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...

journal_title：Experimental cell research

authors： Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C

更新日期：1996-03-15 00:00:00

abstract：:The pre-mRNA splicing factor 4 kinase PRP4K (PRPF4B), is an essential kinase that is a component of the U5 snRNP and functions in spliceosome assembly. We demonstrated that PRP4K is a novel biological marker for taxane response in ovarian cancer patients and reduced levels of PRP4K correlate with intrinsic and acquire...

journal_title：Experimental cell research

authors： Lahsaee S,Corkery DP,Anthes LE,Holly A,Dellaire G

更新日期：2016-01-01 00:00:00

abstract：:The expression level of DNA polymerase beta was determined in various mouse tissues. Northern blot hybridization analysis using rat cDNA as a probe revealed that the mRNA of about 1.5 kb for this enzyme is present in all kinds of tissues examined, but its content widely varies among tissues; the most abundant DNA poly...

journal_title：Experimental cell research

authors： Hirose F,Hotta Y,Yamaguchi M,Matsukage A

更新日期：1989-03-01 00:00:00

abstract：:Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

journal_title：Experimental cell research

authors： deHart GW,Healy KE,Jones JC

更新日期：2003-02-01 00:00:00

abstract：:Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

journal_title：Experimental cell research

authors： Magin TM,Reichelt J,Hatzfeld M

更新日期：2004-11-15 00:00:00

abstract：:We previously showed that the phenotypes of adipocyte progenitors and macrophages were close. Using functional analyses and microarray technology, we first tested whether this intriguing relationship was specific to adipocyte progenitors or could be shared with other progenitors. Measurements of phagocytic activity an...

journal_title：Experimental cell research

authors： Charrière GM,Cousin B,Arnaud E,Saillan-Barreau C,André M,Massoudi A,Dani C,Pénicaud L,Casteilla L

更新日期：2006-10-15 00:00:00

abstract：:Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...

journal_title：Experimental cell research

authors： McFarlane C,Hennebry A,Thomas M,Plummer E,Ling N,Sharma M,Kambadur R

更新日期：2008-01-15 00:00:00