Abstract:
:We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spatiotemporal pattern of Caenorhabditis elegans STIP-1 protein. RNA analyses and Western blots revealed that stip-1 mRNA was produced via trans-splicing and translated as a 95-kDa protein. Using reporter constructs, we found STIP-1 to be expressed at all developmental stages and in many tissue/cell types including worm oocyte nuclei. We found that STIP-1 is targeted to the nucleus and forms large polymers with a rod-like shape when expressed in mammalian cells. Using deletion mutants, we mapped the regions of STIP-1 involved in nuclear import and polymer assembly. We further showed that knockdown of C. elegans stip-1 by RNA interference arrested development and resulted in morphologic abnormalities around the 16-cell stage followed by 100% lethality, suggesting its essential role in worm embryogenesis. Importantly, the embryonic lethal phenotype could be faithfully rescued with Drosophila and human genes via transgenic expression. Our data provide the first direct evidence that STIP have a conserved essential nuclear function across metazoans from worms to humans.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ji Q,Huang CH,Peng J,Hashmi S,Ye T,Chen Ydoi
10.1016/j.yexcr.2007.01.003subject
Has Abstractpub_date
2007-04-15 00:00:00pages
1460-72issue
7eissn
0014-4827issn
1090-2422pii
S0014-4827(07)00007-9journal_volume
313pub_type
杂志文章abstract::Chronic high glucose levels lead to the formation of advanced glycation end-products (AGEs) as well as AGE precursors, such as methylglyoxal (MG) and glyoxal, via non-enzymatic glycation reactions in patients with diabetic mellitus. Glyoxalase 1 (GLO-1) detoxifies reactive dicarbonyls that form AGEs. To investigate th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.10.013
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abstract::L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.10.006
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abstract::Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in t...
journal_title:Experimental cell research
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abstract::Despite an increasing interest in the role of the p53 tumour suppressor protein in embryonic stem cells, not much is known about its regulation in this cell type. We show that the relatively high amount of p53 protein correlates with a higher amount of p53 RNA in ES cells compared to differentiated cells. Moreover, p5...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.05.004
更新日期:2014-09-10 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5376
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90390-9
更新日期:1987-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.06.001
更新日期:2004-09-10 00:00:00
abstract::The Alternative Reading Frame (ARF) protein suppresses tumorigenesis through p53-dependent and p53-independent pathways. Most of ARF's anti-proliferative activity is conferred by sequences in its first exon. Previous work showed specific amino acid changes occurred in that region during primate evolution, so we progra...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2009.01.010
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abstract::Human diploid fibroblasts (HDF) rarely, if ever, undergo spontaneous transformation to an immortalized cell type. Here we report the immortalization of an HDF cell line following transduction with cyclin A2 or cdk1 human genes via retroviral vectors. Fluorescence in situ hybridization (FISH) studies using the retrovir...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.11.030
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5073
更新日期:2001-01-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5277
更新日期:2001-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 评论,杂志文章
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2017.09.036
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pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.12.025
更新日期:2006-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/0014-4827(91)90156-o
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pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4432
更新日期:1999-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00