Abstract:
:Spindle assembly is essential for the equal distribution of genetic material to the daughter cells during mitosis. The process of spindle assembly is complicated and involves multiple levels of molecular regulation. It is generally accepted that mitotic spindles are emanated from the centrosomes and are assembled in the vicinity of chromosomes. However, the molecular mechanism involved in the spindle assembly during mitosis remains unclear. In this study, we have provided several lines of evidence to show that Drosophila Mars is required for the assembly and stabilization of kinetochore microtubules. In an immunocytochemical study, we show that Mars is mainly localized on the kinetochore microtubules during mitosis. Using RNA interference to deplete the Mars expression in Drosophila S2 cells resulted in the malformation of mitotic spindle that mainly lacked the kinetochore microtubules. The spindle defect resulted in mitotic delays by increasing the percentage of uncongressed chromosomes both in vitro and in vivo. In summary, this study has extended our previous study of Mars in cell cycle regulation and provided further evidence showing that Mars is required for the assembly of kinetochore microtubules.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Yang CP,Fan SSdoi
10.1016/j.yexcr.2008.08.004subject
Has Abstractpub_date
2008-10-15 00:00:00pages
3209-20issue
17eissn
0014-4827issn
1090-2422pii
S0014-4827(08)00312-1journal_volume
314pub_type
杂志文章abstract::The ultrastructural localization of adenylate cyclase was studied in bovine cumulus-oocyte complexes. Adenylate cyclase was observed on the plasma membrane of the oocyte and occasionally on the plasma membrane of cumulus cells. The cytochemical observations presented demonstrate that there is more adenylate cyclase in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90149-8
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90190-5
更新日期:1988-04-01 00:00:00
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2018.06.010
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.12.010
更新日期:2014-02-15 00:00:00
abstract::Butyrate and other short-chain fatty acids (SCFA), produced by colonic bacterial flora, affect numerous epithelial cell functions. To better understand how SCFA regulate ion transport, we investigated the effects of 4-phenylbutyrate (4-PBA) on Na(+) absorption in T84 cells. Under standard cell culture conditions, the ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.10.012
更新日期:2007-01-15 00:00:00
abstract::In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4175
更新日期:1998-10-10 00:00:00
abstract::Growth factors and cytokines initiate multiple signal transduction pathways that lead to cell survival, cell cycle progression or differentiation. A common feature of these pathways is increased cellular metabolism and glucose uptake. Furthermore, the energy requirements of many cancers and transformed cell lines are ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.10.027
更新日期:2004-02-15 00:00:00
abstract::Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90404-x
更新日期:1984-04-01 00:00:00
abstract::LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specifi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00018-1
更新日期:2003-04-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.04.013
更新日期:2014-08-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1268
更新日期:1995-08-01 00:00:00
abstract::Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4737
更新日期:2000-01-10 00:00:00
abstract::Melanotransferrin is a glycoprotein expressed at the cell membrane and secreted in the extracellular environment. Recombinant truncated form of membrane-bound melanotransferrin (sMTf) was reported to exert in vitro anti-angiogenic properties. Here we show that sMTf treatment leads to a 50% inhibition of neovasculariza...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.013
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abstract::The program of biochemical and molecular events necessary for commitment to erythroid cell differentiation is particularly well characterized in murine Friend erythroleukemia cell lines. Commitment to hemoglobin synthesis in response to a variety of chemical inducers, including hexamethylene bisacetamide and dimethyl ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4312
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abstract::It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5222
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abstract::An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.05.026
更新日期:2018-08-15 00:00:00
abstract::The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4894
更新日期:2000-07-10 00:00:00
abstract::The effect of the physiological vitamin D metabolite 24R, 25-dihydroxyvitamin D3 [24R,25(OH)2D3] on human osteoblastic cells was assessed. Physiological concentrations (10(-9)-10(-8) M) of 24R, 25(OH)2D3 significantly increased the cyclic guanosine 5'-monophosphate (cGMP) content in the human osteoblastic cells by app...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4189
更新日期:1998-10-10 00:00:00
abstract::Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...
journal_title:Experimental cell research
pub_type: 杂志文章
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abstract::Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.02.011
更新日期:2005-05-15 00:00:00
abstract::In the dystrophic pigmented Royal College of Surgeons (RCS) rat, the retinal pigment epithelium (RPE) has a diminished capacity to phagocytose shed photoreceptor outer segments (ROS). An alteration in phagocytic recognition or ligand-receptor interactions between the RPE and ROS's could contribute to this defect. To t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90174-5
更新日期:1984-10-01 00:00:00
abstract::Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.10.025
更新日期:2005-02-15 00:00:00
abstract::Alzheimer's disease (AD) is characterized by early degeneration of cholinergic neurons and decreased levels of nerve growth factor (NGF). Thus, increasing the NGF levels by for instance encapsulated cell bio-delivery (ECB) is a potential treatment strategy. The results from our previous first-in-human studies on ECB o...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.08.007
更新日期:2018-10-01 00:00:00