Abstract:
:In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable to express a transgene in all the cells of the cutaneous epithelium. Since no ubiquitously expressed promoter sequences had previously been identified, we used lacZ reporter transgenes to test two enhancer/promoter sequences for ubiquitous expression in the skin of adult transgenic mice. We find that a CMV enhancer/CMV promoter is not active in most cell types in the skin, whereas a CMV enhancer/modified beta-actin promoter sequence is active in the suprabasal and basal cells of the epidermis as well as in the epithelial cells of the hair follicles, sebaceous glands, and the dermal papillae.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Sawicki JA,Morris RJ,Monks B,Sakai K,Miyazaki Jdoi
10.1006/excr.1998.4175subject
Has Abstractpub_date
1998-10-10 00:00:00pages
367-9issue
1eissn
0014-4827issn
1090-2422pii
S0014482798941751journal_volume
244pub_type
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.4928
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