Characteristics of intracellular transferrin produced by human fibroblasts: its posttranscriptional regulation and association with tubulin.

abstract：:The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spon...

journal_title：Experimental cell research

authors： Raskó I,Péter SL,Dallmann L,Bajszár G,Péter S,Burg K

更新日期：1978-05-01 00:00:00

abstract：:Bcl-2 family proteins regulate apoptosis at the level of mitochondria. To examine the mechanism of Bcl-2 function, we investigated the effects of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) on two hematopoietic cell lines and Bcl-2 overexpressing transfectants. CCCP directly interferes with mitoc...

journal_title：Experimental cell research

authors： de Graaf AO,van den Heuvel LP,Dijkman HB,de Abreu RA,Birkenkamp KU,de Witte T,van der Reijden BA,Smeitink JA,Jansen JH

更新日期：2004-10-01 00:00:00

abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

journal_title：Experimental cell research

authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

更新日期：1990-09-01 00:00:00

abstract：:Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

journal_title：Experimental cell research

authors： Tiernan AR,Champion JA,Sambanis A

更新日期：2015-01-01 00:00:00

abstract：:The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...

journal_title：Experimental cell research

authors： Pedersen SF,Mills JW,Hoffmann EK

更新日期：1999-10-10 00:00:00

abstract：:Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

journal_title：Experimental cell research

authors： Yuan A,Chia CP

更新日期：2000-12-15 00:00:00

abstract：:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...

journal_title：Experimental cell research

authors： Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

更新日期：2004-05-01 00:00:00

abstract：:Human endothelial cells, when suspended within three-dimensional collagen matrices, develop intracellular vacuoles that coalesce to form capillary lumens and tubes. Vacuole and lumen formation are completely dependent on the collagen-binding integrin alpha 2 beta 1, while other endothelial cell integrins had no appare...

journal_title：Experimental cell research

authors： Davis GE,Camarillo CW

更新日期：1996-04-10 00:00:00

abstract：:Pigmentation of RVH 421 human melanoma cells is induced when cell division is inhibited by cytochalasin D or L-tyrosine phosphate. Increased pigmentation correlates with increased tyrosinase activity when this is monitored over a time-course. Parallel measurements show that the amount of tyrosinase mRNA correlates wit...

journal_title：Experimental cell research

authors： Winder AJ,Harris H

更新日期：1992-04-01 00:00:00

abstract：:We had earlier demonstrated that a 46-kDa glycoprotein is involved in the differentiation of rat skeletal myoblasts. We now show that the binding of this glycoprotein to collagen and gelatin is disrupted by Arg-Gly-Asp (RGD) containing peptide but not by Arg-Gly-Glu (RGE). The former peptide also selectively elutes th...

journal_title：Experimental cell research

authors： Nandan D,Cates GA,Ball EH,Sanwal BD

更新日期：1988-11-01 00:00:00

abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...

journal_title：Experimental cell research

authors： Vasilikaki-Baker H,Nishioka Y

更新日期：1983-08-01 00:00:00

abstract：:Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...

journal_title：Experimental cell research

authors： Jeansson S,Brorson JE

更新日期：1985-11-01 00:00:00

abstract：:The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

journal_title：Experimental cell research

authors： Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

更新日期：2008-04-01 00:00:00

abstract：:Heat shock proteins (HSPs) have molecular chaperone functions in protein biogenesis as well as cytoprotective functions against deleterious environmental stresses, and they work mainly inside of the cells. HSPs are usually induced in living cells that have been exposed to mild stresses or have recovered from severe st...

journal_title：Experimental cell research

authors： Saito K,Dai Y,Ohtsuka K

更新日期：2005-10-15 00:00:00

abstract：:Chitosan oligosaccharides (COS), linear polymers of N-acetyl-D-glucosamine and deacetylated glucosamine, exhibit diverse pharmacological effects such as antimicrobial, antitumor, antioxidant and anti-inflammatory activities. Here, we explored their hypocholesterolemic effects in vivo and the molecular mechanisms of CO...

journal_title：Experimental cell research

authors： Yang X,Zhang J,Chen L,Wu Q,Yu C

更新日期：2018-05-15 00:00:00

abstract：:The stroma, the middle layer of the cornea, is a connective tissue making up most of the corneal thickness. The stromal extracellular matrix (ECM) consists of highly organised lamellae which are made up of tightly packed fibrils primarily composed of collagens type I and V. This layer is interspersed with keratocytes,...

journal_title：Experimental cell research

authors： Greene CA,Green CR,Dickinson ME,Johnson V,Sherwin T

更新日期：2016-09-10 00:00:00

abstract：:Diabetic nephropathy (DN) is a major microvascular complication of diabetes that can lead to end-stage renal disease. Podocytes constitute the last barrier of glomerular filtration, whose damage are the direct cause of proteinuria. Dopamine receptors are involved in the regulation of diabetes-induced glomerular hyperf...

journal_title：Experimental cell research

authors： Shao X,Zhang X,Hu J,Gao T,Chen J,Xu C,Wei C

更新日期：2020-03-15 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:Hematopoiesis is the hierarchical process in which all lineages of blood cells are produced by self-renewing hematopoietic stem cells (HSCs) in the bone marrow (BM). While the regulatory factors that maintain proper HSC function and lineage output under normal conditions are well understood, significantly less is know...

journal_title：Experimental cell research

authors： Mirantes C,Passegué E,Pietras EM

更新日期：2014-12-10 00:00:00

abstract：:Giant axonal neuropathy skin fibroblasts, which are characterized by a selective and partial disorganization of vimentin filaments [1] exhibited, when compared with normal skin fibroblasts, less fibrin clot retractile (FCR) activity and spreading within the fibrin clot both during active growth and resting stage. Skin...

journal_title：Experimental cell research

authors： Azzarone B,Macieira-Coelho A

更新日期：1984-11-01 00:00:00

abstract：BACKGROUND:Esophageal fibrosis is a complication of eosinophilic esophagitis (EoE) which has been attributed to both subepithelial fibrosis and to epithelial to mesenchymal transition (EMT), a process by which epithelial cells acquire mesenchymal features. Common to both causes of EoE-fibrosis is the notion that granul...

journal_title：Experimental cell research

authors： Muir AB,Lim DM,Benitez AJ,Modayur Chandramouleeswaran P,Lee AJ,Ruchelli ED,Spergel JM,Wang ML

更新日期：2013-04-01 00:00:00

abstract：:The Chinese hamster dihydrofolate reductase (DHFR) origin consists of many inefficient initiation sites scattered throughout a 55-kb intergenic spacer, with the ori-beta, ori-beta', and ori-gamma subregions being preferred. To test for the presence of genetic replicators within these subregions, fragments containing o...

journal_title：Experimental cell research

authors： Lin HB,Dijkwel PA,Hamlin JL

更新日期：2005-08-01 00:00:00

abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...

journal_title：Experimental cell research

authors： Li J,Liu G,Li L,Yao Z,Huang J

更新日期：2020-04-15 00:00:00

abstract：:Mesenchymal stem cells (MSCs) derived from bone marrow (BM) represents a useful source of adult stem cells for cell therapy and tissue engineering. MSCs are present at a low frequency in the BM; therefore expansion is necessary before performing clinical studies. Fetal bovine serum (FBS) as a nutritional supplement fo...

journal_title：Experimental cell research

authors： Fani N,Ziadlou R,Shahhoseini M,Baghaban Eslaminejad M

更新日期：2016-06-10 00:00:00

abstract：:In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is invo...

journal_title：Experimental cell research

authors： Zhai L,Kita K,Wano C,Wu Y,Sugaya S,Suzuki N

更新日期：2005-05-01 00:00:00

abstract：:Sperm cells from control donors of proven fertility and men from barren couples were studied by conventional procedures, i.e., light microscopy as well as flow cytometry. Light microscopy analysis of semen included the measurement of spermatozoa concentration, morphology, and motility. All the men from barren couples ...

journal_title：Experimental cell research

authors： Troiano L,Granata AR,Cossarizza A,Kalashnikova G,Bianchi R,Pini G,Tropea F,Carani C,Franceschi C

更新日期：1998-06-15 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are multifunctional signaling molecules that have gained increasing interest in cancer research. To obtain a systematic view on BMP signaling in pancreatic cancer we first determined the mRNA expression levels of seven BMP ligands (BMP2-BMP8) and six BMP specific receptors in pancrea...

journal_title：Experimental cell research

authors： Virtanen S,Alarmo EL,Sandström S,Ampuja M,Kallioniemi A

更新日期：2011-09-10 00:00:00

abstract：:Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM i...

journal_title：Experimental cell research

authors： Xu Y,Sun W,Zheng B,Liu X,Luo Z,Kong Y,Xu M,Chen Y

更新日期：2019-06-01 00:00:00

abstract：:Gp38k is a 383-amino-acid secreted glycoprotein expressed by cultured vascular smooth muscle cells during the time of transition from a proliferating monolayer culture to a nonproliferating multilayered (differentiated) culture. Expression continues as the cell culture forms multicellular nodules. Because this transit...

journal_title：Experimental cell research

authors： Malinda KM,Ponce L,Kleinman HK,Shackelton LM,Millis AJ

更新日期：1999-07-10 00:00:00

abstract：:The enzyme activity hydrolysing diadenosine 5,5'-P1, P4-tetraphosphate (AP4A) was demonstrated in the embryonic extract of sea urchin. The enzyme activity was preferentially inhibited by ZnCl2 and by high concentrations of isobutylmethylxanthine, indicating that two types of the enzyme, (AP4A) hydrolase and non-specif...

journal_title：Experimental cell research

authors： Morioka M,Shimada H

更新日期：1987-03-01 00:00:00