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Cell-cycle-dependent regulation of cell motility and determination of the role of Rac1.

Abstract：

:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three key parameters describing cell motility: speed, persistence time and rate of diffusion. All investigated cell lines demonstrated a lower cell displacement in the G2 phase than in the G1/S phases. This was caused by a decrease in speed and/or persistence time. The decrease in motility was accompanied by changes in morphology reflecting the larger volume of cells in G2 than in G1. Furthermore, L-cells and HeLa-cells appeared to be less adherent in the G2 phase. Transfection of L-cells with constitutively active Rac1 led to a general increase in the speed and rate of diffusion in G2 to levels comparable to those of control cells in G1. In contrast, transfection with dominant-negative Rac1 reduced cell speed and resulted in cellular displacements, which were identical in G1 and G2. These observations indicate that migration of cultured cells is regulated in a cell-cycle-dependent manner, and that an enhancement of Rac1 activity is sufficient for a delay of the reduced cell displacement otherwise seen in G2.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

doi

10.1016/j.yexcr.2004.01.011

keywords:

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

407-20

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482704000321

journal_volume

295

pub_type

杂志文章

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