Abstract：
:The distribution of rDNA and the uptake of tritiated uridine was investigated in nucleoli of human Sertoli cells. The nucleolar components in these cells are spatially arranged in a highly ordered and invariable way and can be recognized in both light and electron microscopy. The pattern of distribution of rDNA and the pattern of uridine uptake in these nucleoli correspond to the distribution of the dense fibrillar component but cannot be correlated to the shape and size of the fibrillar centers in these cells. It therefore can be concluded that the dense fibrillar component, and not the fibrillar centers, is the site of rDNA location and transcription in nucleoli of human Sertoli cells.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wachtler F,Hartung M,Devictor M,Wiegant J,Stahl A,Schwarzacher HGdoi
10.1016/0014-4827(89)90364-9subject
Has Abstractpub_date
1989-09-01 00:00:00pages
61-71issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(89)90364-9journal_volume
184pub_type
杂志文章abstract：:Nuclear and territorial positioning of p- and q-telomeres and centromeres of chromosomes 3, 8, 9, 13, and 19 were studied by repeated fluorescence in situ hybridization, high-resolution cytometry, and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00208-8
更新日期：2003-09-10 00:00:00
abstract：:Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellul...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.05.015
更新日期：2008-08-15 00:00:00
abstract：:Recently, SIRT5 was reported to be a predominant desuccinylase and demalonylase in mitochondria. Ablation of SIRT5 enhances the systemic succinylation and malonylation of mitochondrial proteins, including various metabolic enzymes; however, its function in pancreatic β cells has not yet been clarified. In this study, ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.08.011
更新日期：2018-10-01 00:00:00
abstract：:Rabbit smooth muscle cells (SMC) in primary culture attached to and started proliferating on native and heat-denatured type I collagens, although the amount of cell attachment to denatured collagen was significantly lower. The cells adhered poorly and were unable to grow on commercial gelatin. In contrast, synthetic S...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1225
更新日期：1995-07-01 00:00:00
abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00115-0
更新日期：2003-07-01 00:00:00
abstract：:Plasminogen activation is observed in the human epidermis during reepithelialization of epidermal defects and under certain pathological conditions. The activation reaction depends on keratinocyte-associated plasminogen activators (PAs), which convert the ubiquitous proenzyme plasminogen into the active trypsin-like s...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0061
更新日期：1996-02-25 00:00:00
abstract：:The growth rate of malignant F9 embryonal carcinoma cells slows considerably following all-trans-retinoic acid-induced differentiation into benign parietal endoderm. To determine the mechanism of this process, we examined the expression of cyclins D1, D2, and D3 and the activity of their associated kinases. Cyclin D1 ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4664
更新日期：1999-12-15 00:00:00
abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2005.11.015
更新日期：2006-03-10 00:00:00
abstract：:The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. Polysomal mRNPs were predominantly associated wit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90425-3
更新日期：1987-01-01 00:00:00
abstract：:Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2018.03.023
更新日期：2018-06-01 00:00:00
abstract：:Products of the Neuregulin-1 (Nrg-1) gene, along with the ErbB family of receptor tyrosine kinases through which Nrg-1 ligands signal, play a critical role during cardiovascular development. Through studies of genetically manipulated mice, as well as studies in cells isolated from adult hearts, it appears that Nrg-1/E...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2008.08.015
更新日期：2009-02-15 00:00:00
abstract：:Sensitivity of tumor cells to treatment with anticancer drugs depends on expression and function of antiapoptotic and antioxidant proteins. The goal of our study was to determine the functional role of the novel antioxidant protein Peroxiredoxin V (PrxV), in protection of human lung carcinoma cell lines against apopto...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.05.006
更新日期：2006-09-10 00:00:00
abstract：:Human neutrophils developed long thin tubulovesicular extensions (cytonemes) upon adhesion to fibronectin-coated substrata, when spreading was blocked. We observed extension formation when neutrophils were plated to fibronectin-coated substrata in Na(+)-free extracellular medium or in the presence of drugs capable of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5227
更新日期：2001-06-10 00:00:00
abstract：:Excision of chromatin loop domains and internucleosomal DNA fragmentation are widely considered as consecutive stages of chromatin disassembly during apoptosis. We report here on apoptosis induced by staurosporine in NB-2a neuroblastoma cells, which was accompanied by excision of chromatin loop domains, but proceeded ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.025
更新日期：2007-04-15 00:00:00
abstract：:Hypoxia inducible factor 1 (HIF-1), the key mediator of hypoxia signaling pathways, has been shown involved in hypoxia-induced radioresistance. However, the underlying mechanisms are unclear. The present study demonstrated that both hypoxia and hypoxia mimetic cobalt chloride could increase the radioresistance of huma...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.028
更新日期：2010-07-15 00:00:00
abstract：:Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90495-7
更新日期：1986-10-01 00:00:00
abstract：:In the past, a unique type of precursor for a secretory protein was discovered. It contains a central repetitive domain rich in threonine residues and terminal cysteine-rich domains. Due to striking homologies of these terminal domains with pancreatic spasmolytic polypeptide, originally the name "prepro-spasmolysin" w...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90230-8
更新日期：1990-08-01 00:00:00
abstract：:Cell-CAM 105 is an integral cell surface glycoprotein that is involved in cell-cell adhesion of adult rat hepatocytes in vitro. In the present report we used a radio-immunoassay, a quantitative immunoblotting technique and immunofluorescence microscopy to investigate the expression of cell-CAM 105 in fetal and regener...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90458-1
更新日期：1986-05-01 00:00:00
abstract：:Human lacrimal gland biopsies were serially incubated in chelating and enzymatic solutions. Viable single cells were evaluated for outgrowth in growth factor enriched medium using the following culture substrates: Matrigel, Type I collagen gel with or without fibroblasts, and plastic. Each epithelial outgrowth was cha...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1300
更新日期：1995-09-01 00:00:00
abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0382
更新日期：1996-12-15 00:00:00
abstract：:In chick embryo fibroblasts, the mRNA for extracellular matrix protein tenascin-C is induced 2-fold by cyclic strain (10%, 0.3 Hz, 6 h). This response is attenuated by inhibiting Rho-dependent kinase (ROCK). The RhoA/ROCK signaling pathway is primarily involved in actin dynamics. Here, we demonstrate its crucial impor...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.025
更新日期：2006-05-01 00:00:00
abstract：:The basic helix-loop-helix/leucine zipper (bHLH/ZIP) microphthalmia-associated transcription factor (MITF) regulates transcription of genes encoding enzymes essential for melanin biosynthesis in melanocytes and retinal pigmented epithelial cells. To determine how MITF activity is regulated, we used the yeast two-hybri...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4803
更新日期：2000-03-15 00:00:00
abstract：:ING1b is the most studied ING family protein and perhaps the most ubiquitously and abundantly expressed. This protein is involved in the regulation of various biological functions ranging from senescence, cell cycle arrest, apoptosis, to DNA repair. ING1b is upregulated by UV irradiation and enhances the removal of bu...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.010
更新日期：2007-05-01 00:00:00
abstract：:We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(02)00036-8
更新日期：2003-04-01 00:00:00
abstract：:The intracellular compartmentation of Ap4A in various growth and cell-cycle stages in mammalian cells was studied by applying a non-aqueous extraction procedure for cell nuclei. In both slowly and in exponentially growing Ehrlich ascites tumour cells from random cultures, more than 75% of the whole cellular Ap4A conte...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90607-5
更新日期：1986-08-01 00:00:00
abstract：:Nano-sized Gram-negative bacterial outer membrane vesicles possess unique structural and immunostimulatory effects that could be exploited to regress tumors by alerting the host immune system and reversing the immunosuppressive tumor microenvironment. The current study was conducted to investigate the antitumor activi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112423
更新日期：2021-02-01 00:00:00
abstract：:We examined the relationship of cellular oncogene c-myc and transferrin receptor (TfR) gene expression to cell proliferation and cell cycle progression during myeloid differentiation in the HL-60 myeloid leukemia cell line. In order to determine levels of mRNA for these genes in HL-60 cells induced to differentiate al...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90202-l
更新日期：1990-01-01 00:00:00
abstract：:The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluoresc...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90145-x
更新日期：1992-01-01 00:00:00
abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2020.111925
更新日期：2020-04-15 00:00:00
abstract：:We developed an improved method for isolation of peroxisome biogenesis-defective somatic animal cell mutants, using a combination of green fluorescent protein (GFP) expression and the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) selection method. We used TKaG1 and TKaG2 cells, the wild-type Chinese hamster ovary (CHO) c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4413
更新日期：1999-05-01 00:00:00
