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Characterization of apoptotic pathway associated with caspase-independent excision of DNA loop domains.

Abstract：

:Excision of chromatin loop domains and internucleosomal DNA fragmentation are widely considered as consecutive stages of chromatin disassembly during apoptosis. We report here on apoptosis induced by staurosporine in NB-2a neuroblastoma cells, which was accompanied by excision of chromatin loop domains, but proceeded without internucleosomal DNA cleavage. In contrast to apoptosis associated with internucleosomal DNA fragmentation, the apoptotic pathway associated with excision of chromatin loop domains was largely caspase independent. We identify here MAPK family member, p38/JNK, mitochondria, and topoisomerase II as the components of this caspase-independent apoptotic pathway. While caspase-independent excision of chromatin loop domains was a predominant mechanism of DNA disintegration in staurosporine-treated neuroblastoma, both caspase-dependent internucleosomal DNA fragmentation and caspase-independent excision of chromatin loop domains accompanied staurosporine-induced apoptosis of promyelocytic leukemia cells. Our results suggest that caspase-independent excision of chromatin loop domains represents a separate cell death pathway, which operates either in parallel or independently from caspase-dependent internucleosomal DNA fragmentation.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Solovyan VT

doi

10.1016/j.yexcr.2007.01.025

subject

Has Abstract

pub_date

2007-04-15 00:00:00

pages

1347-60

issue

7

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(07)00045-6

journal_volume

313

pub_type

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