Abstract:
:Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolate uptake into MA104 cells (ED50 = 150 nM). The inhibitory effect was reversed within 30 min after removal of the drug from the cells. Bafilomycin A1 had no effect on the binding of folic acid to its receptor. A concentration of up to 200 nM bafilomycin A1 did not affect sequestration of folate receptors. Immunoblotting showed that the 70-kDa subunit of the V-type proton pump was localized to caveolae-rich fractions isolated from the plasma membrane of these cells. These results suggest that a V-type proton pump acidifies the lumen of plasmalemmal vesicles during potocytosis.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Mineo C,Anderson RGdoi
10.1006/excr.1996.0133subject
Has Abstractpub_date
1996-05-01 00:00:00pages
237-42issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(96)90133-0journal_volume
224pub_type
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