A vacuolar-type proton ATPase mediates acidification of plasmalemmal vesicles during potocytosis.

Abstract:

:Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolate uptake into MA104 cells (ED50 = 150 nM). The inhibitory effect was reversed within 30 min after removal of the drug from the cells. Bafilomycin A1 had no effect on the binding of folic acid to its receptor. A concentration of up to 200 nM bafilomycin A1 did not affect sequestration of folate receptors. Immunoblotting showed that the 70-kDa subunit of the V-type proton pump was localized to caveolae-rich fractions isolated from the plasma membrane of these cells. These results suggest that a V-type proton pump acidifies the lumen of plasmalemmal vesicles during potocytosis.

journal_name

Exp Cell Res

authors

Mineo C,Anderson RG

doi

10.1006/excr.1996.0133

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

237-42

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(96)90133-0

journal_volume

224

pub_type

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