Abstract：
:To study the assembly of intermediate filaments in vivo we have transfected fibroblast cell lines with the cDNAs coding for keratins 8 and 18 under the control of the promoter of the SV40 early region and followed keratin expression by RNA hybridization, two-dimensional gel electrophoresis, and immunofluorescence analysis. When expressed individually, keratins 8 and 18 failed to polymerize into intermediate filaments but formed granular aggregates of variable size distributed throughout the cytoplasm as seen by staining with specific antibodies. The expression of one of these two keratins did not induce the synthesis of its partner or of any other keratin. Coexpression of the two keratins produced filamentous structures, frequently perinuclear, indicating that the two types of polypeptides were able to assemble into intermediate filaments but could not form the cytoskeleton characteristic of epithelial cells. These results demonstrate that assembly in heterocomplexes stabilizes keratins against cellular degradation, helping to explain why excess pools of simple keratins have never been detected.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Domenjoud L,Jorcano JL,Breuer B,Alonso Adoi
10.1016/0014-4827(88)90274-1subject
Has Abstractpub_date
1988-12-01 00:00:00pages
352-61issue
2eissn
0014-4827issn
1090-2422journal_volume
179pub_type
杂志文章abstract：:The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5645
更新日期：2002-11-15 00:00:00
abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111986
更新日期：2020-05-15 00:00:00
abstract：:The aim of the present study was to determine whether mitochondrial activity could regulate myoblast proliferation. We demonstrate that an increase in mitochondrial activity of L6E9 myoblasts can be easily obtained by simply raising extracellular pyruvate concentration in the culture dish. Under this condition, L6E9 m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.05.017
更新日期：2004-09-10 00:00:00
abstract：:Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage respon...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2014.07.003
更新日期：2014-11-15 00:00:00
abstract：:Skeletal muscles cope with a large range of activities, from being able to support the body weight during long periods of upright standing to perform explosive movements in response to an unexpected threat. This requires systems for energy metabolism that can provide energy during long periods of moderately increased ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2010.05.019
更新日期：2010-11-01 00:00:00
abstract：:The relationship between Lamin B2 and tumor proliferation and migration is unclear. We explored the impact of Lamin B2 on non-small cell lung cancer (NSCLC) cells. Tissue microarray and immunohistochemistry were combined to evaluate Lamin B2 expression and its relationship with the clinicopathological factors found in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112090
更新日期：2020-08-15 00:00:00
abstract：:CUB domains are most exclusively found in secreted proteins and in a few transmembrane proteins. These domains are approximately 110 amino acids long and have four conserved cysteines that form a β-sandwich fold. CUB domains proteins are involved in a wide range of biological functions. We have shown that CUB domains ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111499
更新日期：2019-10-01 00:00:00
abstract：:A large number of intracellular proteins are degraded by the ubiquitin-proteasome system, one of the major protein degradation pathways. It produces peptides of several different sizes through protein degradation, and these peptides are rapidly degraded into free amino acids by various intracellular aminopeptidases. P...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.112337
更新日期：2020-12-01 00:00:00
abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2020.111925
更新日期：2020-04-15 00:00:00
abstract：:The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.07.021
更新日期：2011-10-15 00:00:00
abstract：:The intracellular ATP concentration decides on the onset of either apoptosis or necrosis in Jurkat cells exposed to death stimuli. Bcl-2 can block apoptotic demise, which occurs preferably under conditions of high cellular ATP levels. Here, we investigated the effects of Bcl-2 on the necrotic type of cell demise that ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.5059
更新日期：2001-01-01 00:00:00
abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90583-5
更新日期：1986-08-01 00:00:00
abstract：:The centromere is a specialized region of the eukaryotic chromosome that is responsible for directing chromosome movements in mitosis and for coordinating the progression of mitotic events at the crucial transition between metaphase and anaphase. In this review, we will focus on recent advances in the understanding of...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1998.4278
更新日期：1999-02-01 00:00:00
abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.006
更新日期：2005-02-15 00:00:00
abstract：:We have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba2+-stimulated secretion. A specific secretory granule membrane constituent, dopamine-beta-hydroxylase (DBH), has been investigated using immunoprecipitation followed...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90249-8
更新日期：1989-06-01 00:00:00
abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0098
更新日期：1996-03-15 00:00:00
abstract：:The level of integrin alpha(v)beta3 and its ligand osteopontin (OPN) has been directly correlated to tumorigenicity of melanoma and other cancer cells. We have previously shown an increase in pp(60c-Src) kinase activity associated with integrin alpha(v)beta3 in melanoma cells (M21) treated with soluble OPN. pp(60c-Src...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.03.022
更新日期：2006-07-15 00:00:00
abstract：:Advanced stage neuroblastomas (NB) exhibit a tissue inhibitor of metalloproteinase (TIMP)-2/matrix metalloproteinase (MMP) imbalance, considered a prerequisite for MMP involvement in tumor progression in vivo. Human SH-SY5Y NB cells exhibit a similar TIMP-2/MMP imbalance that promotes in vitro invasive behavior that i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00072-7
更新日期：2003-06-10 00:00:00
abstract：:The NADH-linked substrates pyruvate, L-glutamine, and beta-hydroxybutyrate, while enhancing the rate of oxygen consumption, also increased the formation of DNA single-strand breaks induced by tert-butylhydroperoxide in intact U937 cells. A cause-effect relationship between these two parameters was established by showi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3779
更新日期：1997-11-25 00:00:00
abstract：:The extracellular matrix in the healthy breast has an important tumor suppressive role, whereas the abnormal ECM in tumors can promote aggressiveness, and has been linked to breast cancer relapse, survival and resistance to chemotherapy. This review article gives an overview of the elements of the ECM which have been ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2015.11.009
更新日期：2016-04-10 00:00:00
abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4381
更新日期：1999-03-15 00:00:00
abstract：:Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in China with a poor prognosis. Most ESCC patients were diagnosed at advanced stages, losing the opportunity for surgical excision. Hence, it remains a pressing work to identify biomarkers for early detection, prognosis prediction and target...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.03.017
更新日期：2018-06-01 00:00:00
abstract：:The specific action of a pig skin fraction enriched in epidermal G1-chalone, a tissue-specific inhibitor of epidermal DNA synthesis, was investigated by means of flow cytofluorometry. The results indicate that G1-chalone inhibits progression of partially synchronized rat tongue epithelial cells (line RTE-2) through th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90235-8
更新日期：1989-06-01 00:00:00
abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2017.02.024
更新日期：2017-07-15 00:00:00
abstract：:The Ras-like family of small GTPases includes, among others, Ras, Rap1, R-ras, and Ral. The family is characterized by similarities in the effector domain. While the function of Ras is, at least in part, elucidated, little is known about other members of the family. Currently, much attention is focused on the small GT...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1999.4695
更新日期：1999-11-25 00:00:00
abstract：:UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.07.017
更新日期：2010-10-15 00:00:00
abstract：:The beta 1-integrin subunit localizes to focal contacts during F9 teratocarcinoma stem cell differentiation to parietal endoderm. Concomitantly, this integrin subunit becomes dephosphorylated at serine 790, the only serine in the well-conserved cytoplasmic domain of beta 1. We hypothesized that this dephosphorylation ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0002
更新日期：1996-01-10 00:00:00
abstract：:Transcription factors belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH/PAS) family control a wide variety of biological processes in mammalian and/or Drosophila. We have previously isolated bHLH/PAS Xenopus amphibian homologs of Single-minded (xSim) and aryl receptor nuclear translocator (xArnt) and characte...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00051-x
更新日期：2003-07-15 00:00:00
abstract：:A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times....
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90369-0
更新日期：1984-03-01 00:00:00
abstract：:Breast cancer and prostate cancer are the most common malignant tumors in female and men, respectively. Furthermore, lung cancer is the leading cause of cancer deaths worldwide. It is an emergency to develop a powerful strategy to treat these threatening cancers more effectively, because of low efficacy and high rates...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2018.03.023
更新日期：2018-06-01 00:00:00
