Abstract:
:We have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba2+-stimulated secretion. A specific secretory granule membrane constituent, dopamine-beta-hydroxylase (DBH), has been investigated using immunoprecipitation followed by electrophoresis. Within 30 min of stimulation, exposed DBH had been cleared from the cell surface. Nevertheless, quantitation of labeled DBH using [125I] streptavidin suggested that it remained undegraded over a period of 24 h, a time during which secretory granule stores of catecholamines were being replenished. Subcellular fractionation of the cultured cells suggested that, after 3 or 4 h, the biotinylated DBH, which was still membrane-bound, was located in particulate material that also contained cytochrome b561, another major secretory granule membrane component.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Hunter A,Phillips JHdoi
10.1016/0014-4827(89)90249-8subject
Has Abstractpub_date
1989-06-01 00:00:00pages
445-60issue
2eissn
0014-4827issn
1090-2422journal_volume
182pub_type
杂志文章abstract::Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.04.031
更新日期:2010-09-10 00:00:00
abstract::The fruit-fly Drosophila melanogaster harbours different types of ciliary structures: ciliary projections associated with neurons of type I and cilium-like regions (CLRs) found during male gametogenesis. The latter deserve particular attention since they are morphologically similar to vertebrate primary cilia and tran...
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:1987-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90438-z
更新日期:1991-12-01 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:1999-12-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:1996-02-25 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00