The role of phosphorylation in modulating beta 1 integrin localization.

abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

journal_title：Experimental cell research

authors： Vaughan AT,Gordon DJ

更新日期：1992-10-01 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF), a progressive disorder of unknown etiology, is characterized by pathological lung fibroblast activation and proliferation resulting in abnormal deposition of extracellular matrix proteins within the lung parenchyma. The pathophysiological roles of exosomal microRNAs in pulmonary fi...

journal_title：Experimental cell research

authors： Inomata M,Kamio K,Azuma A,Matsuda K,Usuki J,Morinaga A,Tanaka T,Kashiwada T,Atsumi K,Hayashi H,Fujita K,Saito Y,Kubota K,Seike M,Gemma A

更新日期：2021-01-15 00:00:00

abstract：:3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...

journal_title：Experimental cell research

authors： Tamgüney T,Zhang C,Fiedler D,Shokat K,Stokoe D

更新日期：2008-07-01 00:00:00

abstract：:Although periodontal diseases are initiated by bacteria that colonize the tooth surface and gingival sulcus, the host response is believed to play an essential role in the breakdown of connective tissue and bone. Mitochondrial reactive oxygen species (mtROS) have been proposed to regulate the activation of the inflamm...

journal_title：Experimental cell research

authors： Li X,Wang X,Zheng M,Luan QX

更新日期：2016-09-10 00:00:00

abstract：:The mechanisms that structure the mammalian endoplasmic reticulum (ER) network are not fully understood. Here we show that salt extraction of semi-intact normal rat kidney (NRK) fibroblasts and subsequent incubation of the extracted cells with ATP resulted in dramatic ER network retraction. Under these conditions, add...

journal_title：Experimental cell research

authors： Baughman C,Morin-Leisk J,Lee T

更新日期：2008-08-15 00:00:00

abstract：:We performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) on control and TGF-β1-exposed rat lung fibroblasts to identify proteins differentially expressed between cell populations. A total of 196 proteins were found to be differentially expressed in response to TGF-β1 treatment. Guided by these results, w...

journal_title：Experimental cell research

authors： Na M,Hong X,Fuyu J,Dingjie X,Sales D,Hui Z,Zhongqiu W,Shifeng L,Xuemin G,Wenchen C,Dan L,Guizhen Z,Bonan Z,Lijuan Z,Shumin L,Ying Z,Jin W,Mingwang R,Summer R,Fang Y

更新日期：2019-02-15 00:00:00

abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...

journal_title：Experimental cell research

authors： Yokota T,Ehlin-Henriksson B,Hansson GK

更新日期：1998-02-25 00:00:00

abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

journal_title：Experimental cell research

authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

更新日期：1996-12-15 00:00:00

abstract：:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled wi...

journal_title：Experimental cell research

authors： Nakamura H,Morita T,Sato C

更新日期：1986-08-01 00:00:00

abstract：:Several species of Streptococcus, such as S. salivarius, S. mitis, and S. anginosus, are found to extensively colonize the oral cavity and the upper respiratory tract, and have been shown to increase in patients with oral squamous cell carcinoma (OSCC). Accumulating evidence have revealed that commensal bacteria are i...

journal_title：Experimental cell research

authors： Wang J,Sun F,Lin X,Li Z,Mao X,Jiang C

更新日期：2018-01-01 00:00:00

abstract：:Most cases of familial early-onset Alzheimer's disease are caused by mutations in the presenilin 1 (PS1) gene. However, the cellular functions of PS1 are unknown. We showed predominant localization of PS1 to cell-cell contacts of the plasma membrane in human prostate epithelial tissue and in a human epithelial cell li...

journal_title：Experimental cell research

authors： Singh N,Talalayeva Y,Tsiper M,Romanov V,Dranovsky A,Colflesh D,Rudamen G,Vitek MP,Shen J,Yang X,Goldgaber D,Schwarzman AL

更新日期：2001-02-01 00:00:00

abstract：:Human calmodulin-like protein (CLP) is a calcium-binding protein down-regulated in a cell culture model of mammary tumorigenesis as well as in a majority of breast cancers in vivo. CLP down-regulation may be a result of the poorly differentiated state of these cell lines and tumors, or CLP expression may be incompatib...

journal_title：Experimental cell research

authors： Rogers MS,Kobayashi T,Pittelkow MR,Strehler EE

更新日期：2001-07-15 00:00:00

abstract：:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...

journal_title：Experimental cell research

authors： Lindahl PE

更新日期：1978-05-01 00:00:00

abstract：:The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...

journal_title：Experimental cell research

authors： Hellberg C,Eierman D,Sjölander A,Andersson T

更新日期：1995-03-01 00:00:00

abstract：:Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...

journal_title：Experimental cell research

authors： Marchand E,Constantin B,Balghi H,Claudepierre MC,Cantereau A,Magaud C,Mouzou A,Raymond G,Braun S,Cognard C

更新日期：2004-07-15 00:00:00

abstract：:The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...

journal_title：Experimental cell research

authors： Pedersen SF,Mills JW,Hoffmann EK

更新日期：1999-10-10 00:00:00

abstract：:Chemoresistance is a severe limitation to glioblastoma (GBM) therapy and there is a strong need to understand the underlying mechanisms that determine its response to different chemotherapeutics. Therefore, we induced resistance in C6 rat glioma cell line, which considerably resembles the characteristics of human GBM....

journal_title：Experimental cell research

authors： Stojković S,Podolski-Renić A,Dinić J,Stanković T,Banković J,Hadžić S,Paunović V,Isaković A,Tanić N,Pešić M

更新日期：2015-07-15 00:00:00

abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

journal_title：Experimental cell research

authors： Broadley C,Dawidowicz E,Chong PL,Hoover R

更新日期：1991-03-01 00:00:00

abstract：:The histone 2A variant macroH2A1.2 is expressed in female and male mammals and is implicated in X-chromosome inactivation and autosomal gene silencing. In undifferentiated and early differentiating murine embryonic stem (ES) cells a cytosolic pool of macroH2A1.2 has recently been reported and found to be associated wi...

journal_title：Experimental cell research

authors： Mermoud JE,Tassin AM,Pehrson JR,Brockdorff N

更新日期：2001-08-15 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...

journal_title：Experimental cell research

authors： Schonberg S,Patterson D,Puck TT

更新日期：1983-04-15 00:00:00

abstract：:Natural BH3-memitic (-)-gossypol shows promising antitumor efficacy in several kinds of cancer. However, our previous studies have demonstrated that protective autophagy decreases the drug sensitivities of Bcl-2 inhibitors in hepatocellular carcinoma (HCC) cells. In the present study, we are the first to report that H...

journal_title：Experimental cell research

authors： Wang B,Chen L,Ni Z,Dai X,Qin L,Wu Y,Li X,Xu L,Lian J,He F

更新日期：2014-11-01 00:00:00

abstract：:The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

journal_title：Experimental cell research

authors： Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

更新日期：1988-07-01 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) induces apoptosis in fetal rat hepatocytes. However, a subpopulation of these cells survives, concomitant with changes in phenotype, reminiscent of an epithelial-mesenchymal transition (EMT). We have previously suggested that EMT might confer cell resistance to apoptosis (Val...

journal_title：Experimental cell research

authors： Del Castillo G,Murillo MM,Alvarez-Barrientos A,Bertran E,Fernández M,Sánchez A,Fabregat I

更新日期：2006-09-10 00:00:00

abstract：:Skin fibroblasts from patients with inherited adenomatosis of the large bowel (ACR-SF) possess alterations in actin microfilament (MF) organization which serve to distinguish "predisposed" cells from fibroblasts derived from normal individuals (NSF). MF bundle frequency and diameter were considerably reduced in ACR-SF...

journal_title：Experimental cell research

authors： Higgins PJ,Kopelovich L

更新日期：1991-08-01 00:00:00

abstract：:Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in the early stage of pancreatic cancer, contributing to the poor prognosis. Accordingly, in this study, we evaluated the molecular mechanisms underlying invasion and metastasis. Using mass spectrometry, we found that Integrin alp...

journal_title：Experimental cell research

authors： Wu Y,Tan X,Liu P,Yang Y,Huang Y,Liu X,Meng X,Yu B,Wu M,Jin H

更新日期：2019-06-01 00:00:00

abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

journal_title：Experimental cell research

authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

更新日期：1997-02-25 00:00:00

abstract：:The cellular plasma membrane becomes permeable to macromolecules during the cell injury process. This results in exposure of the interior of the cell to plasma proteins and to high-affinity binding of the Fc part of IgG to intermediate filaments (Hansson, G K, Starkebaum, G A, Benditt, E P & Schwartz, S M, Proc natl a...

journal_title：Experimental cell research

authors： Hansson GK,Lagerstedt E,Bengtsson A,Heideman M

更新日期：1987-06-01 00:00:00

abstract：:The interaction between the central cell-binding domain (CBD) of fibronectin (FN) and its receptor integrin alpha 5 beta 1 was analyzed by both ligand-binding and cell adhesion assays. The ligands used were a CBD fragment (pCBD) of human plasma fibronectin and its recombinant versions including wild type CBD (wtCBD) a...

journal_title：Experimental cell research

authors： Obara M,Yoshizato K

更新日期：1995-01-01 00:00:00

abstract：:It has been suggested that the protein kinase C zeta (zeta PKC) isoform is involved in mitogenic signaling in Xenopus oocytes and mammalian cells. Thus, the characterization of potential regulatory molecules that bind to zeta PKC is of great interest. We report here the identification by affinity chromatography of tub...

journal_title：Experimental cell research

authors： García-Rocha M,Avila J,Lozano J

更新日期：1997-01-10 00:00:00