Abstract:
:In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled with [3H]thymidine at the limited resolution level of autoradiography. By immunofluorescent staining with antibody against 5-bromodeoxyuridine (BrdU), we succeeded in detecting novel, intranuclear ring-like structures of replicating replicon domains that were organized temporarily during the S phase of mammalian cells with incorporated BrdU.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Nakamura H,Morita T,Sato Cdoi
10.1016/0014-4827(86)90583-5subject
Has Abstractpub_date
1986-08-01 00:00:00pages
291-7issue
2eissn
0014-4827issn
1090-2422journal_volume
165pub_type
杂志文章abstract::Microtubule-binding proteins provide an alternative and vital pathway to the functional diversity of microtubules. Considerable work is still required to understand the complexities of microtubule-associated cellular processes and to identify novel microtubule-binding proteins. In this study, we identify Bcl2-associat...
journal_title:Experimental cell research
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abstract::An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
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abstract::Gallbladder cancer (GBC) is an aggressive malignancy of the bile duct, which is associated with a low (5-year) survival and poor prognosis. The transcription factor HIF-1α is implicated in the angiogenesis, cell survival, epithelial mesenchymal transition (EMT) and invasiveness of GBC. In this study, we have investiga...
journal_title:Experimental cell research
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abstract::Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage respon...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2014-11-15 00:00:00
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journal_title:Experimental cell research
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abstract::Breast cancer is the most common carcinoma that metastasizes to bone. Tumor-produced parathyroid hormone-related protein (PTHrP), a known stimulator of osteoclastic bone resorption, is a major mediator of the osteolytic process in breast cancer. We have previously shown that PTHrP increases breast cancer cell prolifer...
journal_title:Experimental cell research
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abstract::Advanced glycation end-products (AGEs) are proteins/lipids that are glycated upon sugar exposure and are often increased during inflammatory diseases such as osteoarthritis and neurodegenerative disorders. Here, we developed an extracellular matrix (ECM) using glycated type I collagen (ECM-GC), which produced similar ...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2019.06.020
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abstract::Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90209-4
更新日期:1983-09-01 00:00:00
abstract::Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1177
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abstract::K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The ty...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4648
更新日期:1999-11-01 00:00:00
abstract::Abdominal aortic aneurysms (AAA) are characterized by matrix remodeling, elastin degradation, absence of nitric oxide (NO) signaling, and inflammation, influencing smooth muscle cell (SMC) phenotype and gene expression. Little is known about the biomolecular release and intrinsic biomechanics of human AAA-SMCs. NO del...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2019.111589
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.01.008
更新日期:2018-06-01 00:00:00
abstract::The biological mechanisms involved in initiating, coordinating, and ultimately terminating cell-cell adhesion in the stratified epithelium are not well understood at present. This study was designed to elucidate the roles of the muscarinic M3, the nicotinic alpha3, and the mixed muscarinic-nicotinic alpha9 acetylcholi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.12.010
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.12.002
更新日期:2006-04-01 00:00:00
abstract::A monoclonal rat IgM antibody (4C9) raised against F9 embryonal carcinoma cells reacted with fucosyl residues in poly-N-acetyllactosamine-type large carbohydrates of these cells (embryoglycan). The chemical properties and distribution of the antigen resembled those of SSEA-1. The monoclonal antibody was found to inhib...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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abstract::Maturation of MC3T3-E1 osteoblast cells in vitro can be divided into three major stages, namely, proliferation, differentiation, and mineralization. Our microarray analysis identified genes differentially expressed between proliferating and differentiated MC3T3-E1 osteoblastic cells. Immunohistochemical analyses of RN...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2001-03-10 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4253
更新日期:1998-12-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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更新日期:2020-03-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:1990-11-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2016-02-15 00:00:00