Abstract:
:Gallbladder cancer (GBC) is an aggressive malignancy of the bile duct, which is associated with a low (5-year) survival and poor prognosis. The transcription factor HIF-1α is implicated in the angiogenesis, cell survival, epithelial mesenchymal transition (EMT) and invasiveness of GBC. In this study, we have investigated the role of HIF-1α in the pathobilogy of GBC and effect of hispidulin on the molecular events controlled by this transcription factor. We observed that hispidulin caused induction of apoptosis, blockade of growth and cell cycle progression in GBC cells. Our results have demonstrated for the first time that hispidulin-exerted anti-tumor effect involved the suppression of HIF-1α signaling. Hispidulin was found to repress the expression of HIF-1α protein dose-dependently without affecting the HIF-1α mRNA expression. In addition, the inhibition of HIF-1α protein synthesis was revealed to be mediated through the activation of AMPK signaling. Hispidulin also sensitized the tumor cells to Gemcitabine and 5-Fluoroucil by down-regulating HIF-1α/P-gp signaling. Given the low cost and exceedingly safe profile, hispidulin appears to be a promising and novel chemosensitizer for GBC treatment.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Gao H,Xie J,Peng J,Han Y,Jiang Q,Han M,Wang Cdoi
10.1016/j.yexcr.2014.11.021subject
Has Abstractpub_date
2015-03-15 00:00:00pages
236-46issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(14)00524-2journal_volume
332pub_type
杂志文章abstract::Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2013.03.002
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abstract::The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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abstract::Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed t...
journal_title:Experimental cell research
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abstract::Cell union in mating pairs in the ciliate Euplotes raikovi is controlled by a system of multiple mating types which are inherited with alleles codominant at the genetic locus mat and expressed via diffusible mating pheromones. The mating pheromones Er-2, Er-3, and Er-11 were purified from cells homozygous for the mat-...
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journal_title:Experimental cell research
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abstract::We have investigated the genetic stability of NIH-3T3 cells transfected with sequences coding for basic fibroblast growth factor (bFGF) by determining drug resistance and gene amplification potential. Colony-forming experiments and fluctuation analyses showed that the frequency and rate of resistance to N-(phosphonace...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2004.10.006
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journal_title:Experimental cell research
pub_type: 杂志文章
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doi:10.1016/0014-4827(85)90257-5
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doi:10.1016/0014-4827(83)90167-2
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2003.07.007
更新日期:2003-12-10 00:00:00
abstract::Elevated plasma free fatty acids level has been implicated in the development of insulin resistance, inflammation, and endothelial dysfunction in diabetic and nondiabetic individuals. However, the underlying mechanisms still remain to be defined. Herein, we investigated the effect of palmitic acid (PA), the most abund...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.10.037
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.07.007
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doi:10.1016/j.yexcr.2014.04.020
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1006/excr.1994.1294
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abstract::The effect of the physiological vitamin D metabolite 24R, 25-dihydroxyvitamin D3 [24R,25(OH)2D3] on human osteoblastic cells was assessed. Physiological concentrations (10(-9)-10(-8) M) of 24R, 25(OH)2D3 significantly increased the cyclic guanosine 5'-monophosphate (cGMP) content in the human osteoblastic cells by app...
journal_title:Experimental cell research
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