Aberrant expression of basic fibroblast growth factor in NIH-3T3 cells alters drug resistance and gene amplification potential.

abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

journal_title：Experimental cell research

authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

更新日期：1996-12-15 00:00:00

abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

journal_title：Experimental cell research

authors： Sakkas D,Mariethoz E,St John JC

更新日期：1999-09-15 00:00:00

abstract：:Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nu...

journal_title：Experimental cell research

authors： Skare P,Kreivi JP,Bergström A,Karlsson R

更新日期：2003-05-15 00:00:00

abstract：:Products of the Neuregulin-1 (Nrg-1) gene, along with the ErbB family of receptor tyrosine kinases through which Nrg-1 ligands signal, play a critical role during cardiovascular development. Through studies of genetically manipulated mice, as well as studies in cells isolated from adult hearts, it appears that Nrg-1/E...

journal_title：Experimental cell research

authors： Pentassuglia L,Sawyer DB

更新日期：2009-02-15 00:00:00

abstract：:The tumor suppressor function of p53 is disabled in the majority of tumors, either by a point mutation of the p53 gene, or via MDM2-dependent proteasomal degradation. We have screened a chemical library using a cell-based assay and identified a low molecular weight compound named MITA which induced wild-type p53-depen...

journal_title：Experimental cell research

authors： Hedström E,Issaeva N,Enge M,Selivanova G

更新日期：2009-02-01 00:00:00

abstract：:Psoriasis is a chronic inflammatory skin disease severely affecting patients' physical and psychological well-being. Aberrant keratin expression in psoriasis plays a crucial role in keratinocyte dysfunction and disease development. Little is yet known about the mechanism of this keratin dysregulation. VEGF (Vascular e...

journal_title：Experimental cell research

authors： Jiang M,Li B,Zhang J,Hu L,Dang E,Wang G

更新日期：2017-11-15 00:00:00

abstract：:Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...

journal_title：Experimental cell research

authors： Jamalpour M,Bergquist E,Welsh M

更新日期：2020-12-15 00:00:00

abstract：:In this report, we elucidate the role of Na(+)-K+ pump in the regulation of polyamine spermidine (Spd) transport in murine leukemia (L 1210) cells in culture. Ouabain, known to bind extracellularly to the alpha-subunit of the Na(+)-K+ pump, inhibits the pump activity. The L 1210 cells were found to possess ouabain bin...

journal_title：Experimental cell research

authors： Khan NA,Quemener V,Moulinoux JP

更新日期：1992-04-01 00:00:00

abstract：:Hypoxia/reoxygenation (H/R) injury is characterized by microfilament reorganization in cardiomyocytes. Previous studies have shown that myofibrillogenesis regulator-1 (MR-1) is expressed in the myocardium and promotes actin organization in cardiomyocytes. The purpose of this study was to investigate the role of MR-1 i...

journal_title：Experimental cell research

authors： Tao T,Wang X,Liu M,Liu X

更新日期：2015-10-01 00:00:00

abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...

journal_title：Experimental cell research

authors： Sato C,Nishizawa K,Nakayama T,Hirai R,Nakamura H

更新日期：1986-12-01 00:00:00

abstract：:The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...

journal_title：Experimental cell research

authors： Zhao C,Hashiguchi A,Kondoh K,Du W,Hata J,Yamada T

更新日期：2002-05-01 00:00:00

abstract：:Incubating conditions which induced actin paracrystal-like intracellular structures (actin rods) were investigated by using several cell lines. We have found that an incubation of cells of a mouse fibroblastic cell line, C3H-2K, in an isotonic solution of NaCl containing 1 mM MgCl2, 1 mM CaCl2 and 10 mM MES, pH 6.5, i...

journal_title：Experimental cell research

authors： Iida K,Yahara I

更新日期：1986-06-01 00:00:00

abstract：:The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

journal_title：Experimental cell research

authors： Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

更新日期：1991-05-01 00:00:00

abstract：:Transferrin (Tf), an iron-binding protein, was investigated in the cultured human fibroblast which is a major cell type in connective tissues. Tf is a major iron-transporting protein and has an important role in iron metabolism. Using two-dimensional gel electrophoresis, we demonstrated the eight subtypes of Tf produc...

journal_title：Experimental cell research

authors： Kondo T,Sakaguchi M,Namba M

更新日期：1998-07-10 00:00:00

abstract：:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...

journal_title：Experimental cell research

authors： Okuda A,Kimura G

更新日期：1996-03-15 00:00:00

abstract：:ADAMs (a disintegrin and metalloprotease) comprise a family of cell surface proteins with protease and cell-binding activities. Using different forms and fragments of ADAM12 as substrates in cell adhesion and spreading assays, we demonstrated that alpha9beta1 integrin is the main receptor for ADAM12. However, when alp...

journal_title：Experimental cell research

authors： Thodeti CK,Fröhlich C,Nielsen CK,Holck P,Sundberg C,Kveiborg M,Mahalingam Y,Albrechtsen R,Couchman JR,Wewer UM

更新日期：2005-10-01 00:00:00

abstract：:The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...

journal_title：Experimental cell research

authors： Ishii T,Shimizu M,Kanayama Y,Nakada S,Nojima H,Oda K

更新日期：1993-10-01 00:00:00

abstract：:The adenosine analog 5,6 dichloro-1-beta-ribofuranosyl benzimidazole (DRB) inhibits specifically mRNA production in eukaryotic cells, with concomitant alterations in pre-rRNA processing and nucleolar ultrastructure. We have focused our analysis on the effects of drug treatment on rRNA transcription through observation...

journal_title：Experimental cell research

authors： Puvion-Dutilleul F,Nicoloso M,Bachellerie JP

更新日期：1983-06-01 00:00:00

abstract：:In small oocytes of Xenopus laevis, the three most abundant proteins are isolated as basic polypeptides with molecular weights of 48 kD (P48), 43 kD (P43) and 40 kD (P40, also known as transcription factor IIIA). All three proteins share common properties in being able to bind specifically ribosomal 5S RNA molecules a...

journal_title：Experimental cell research

authors： Barrett P,Johnson RM,Sommerville J

更新日期：1984-08-01 00:00:00

abstract：:Raver2 was originally identified as a member of the hnRNP family through database searches revealing three N-terminal RNA recognition motifs (RRMs) bearing highest sequence identity in the RNP sequences to the related hnRNP Raver1. Outside the RRM region, both Raver proteins are quite divergent in sequence except for ...

journal_title：Experimental cell research

authors： Henneberg B,Swiniarski S,Sabine Becke,Illenberger S

更新日期：2010-04-01 00:00:00

abstract：:The interphase nucleus is a structurally ordered, three-dimensional structure, in which specific chromatin domains occupy distinct spatial positions that can, in turn, be modified with changes in cell function. A fundamental goal in developmental neurobiology is the identification of mechanisms that dictate the orderl...

journal_title：Experimental cell research

authors： Martou G,De Boni U

更新日期：2000-04-10 00:00:00

abstract：:Recent studies have demonstrated a central role for the exchange protein activated by cAMP (Epac) in the inhibition of Fcgamma-receptor-mediated phagocytosis and bacterial killing by prostaglandin E(2) (PGE(2)) in macrophages. However, the subcellular localization of Epac, and its primary target Rap1, has yet to be de...

journal_title：Experimental cell research

authors： Brock TG,Serezani CH,Carstens JK,Peters-Golden M,Aronoff DM

更新日期：2008-01-15 00:00:00

abstract：UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

journal_title：Experimental cell research

authors： Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

更新日期：2009-11-15 00:00:00

abstract：:Despite over 30 years of work, the fundamental structure of eukaryotic chromatin remains controversial. Here, we review the roots of this controversy in disparities between results derived from studies of chromatin in nuclei, chromatin isolated from nuclei, and chromatin reconstituted from defined components. Thanks t...

journal_title：Experimental cell research

authors： Grigoryev SA,Woodcock CL

更新日期：2012-07-15 00:00:00

abstract：:Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...

journal_title：Experimental cell research

authors： Davies BR,Steele IA,Edmondson RJ,Zwolinski SA,Saretzki G,von Zglinicki T,O'Hare MJ

更新日期：2003-08-15 00:00:00

abstract：:Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

journal_title：Experimental cell research

authors： Yuan A,Chia CP

更新日期：2000-12-15 00:00:00

abstract：:Based on data from developmental RNA profiles and in situ hybridization, we report a direct examination of the expression of one collagen gene (Dcg1) during drosophila melanogaster life cycle. These studies show, for the first time, that the expression of a collagen gene is both differential and tissue-specific during...

journal_title：Experimental cell research

authors： Le Parco Y,Knibiehler B,Cecchini JP,Mirre C

更新日期：1986-04-01 00:00:00

abstract：:Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...

journal_title：Experimental cell research

authors： Grand RJ,Milner AE,Mustoe T,Johnson GD,Owen D,Grant ML,Gregory CD

更新日期：1995-06-01 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:The subcellular location of a soluble DNA replication-stimulating factor released from lysolecithin-permeabilized cultured mammalian cells was investigated by cytochalasin B (CB) enucleation. In both Chinese hamster ovary cells and baby hamster kidney (BHK) cells, approx. 70% of the total activity of the replication-s...

journal_title：Experimental cell research

authors： Myers CA,Patel PI,Miller MR

更新日期：1983-01-01 00:00:00