Abstract：
:Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nuclear ribonucleoprotein particle (snRNP)-core proteins and p80 coilin-containing Cajal bodies. Treatment with actinomycin D led to largely similar reorganizations of snRNPs and profilin, while profilin and Cajal bodies separated under these conditions. One of the profilin antibodies interfered with pre-mRNA splicing in vitro, further indicating a role for profilin during pre-mRNA processing.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Skare P,Kreivi JP,Bergström A,Karlsson Rdoi
10.1016/s0014-4827(03)00102-2keywords:
subject
Has Abstractpub_date
2003-05-15 00:00:00pages
12-21issue
1eissn
0014-4827issn
1090-2422pii
S0014482703001022journal_volume
286pub_type
杂志文章abstract：:In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.11.025
更新日期：2006-04-01 00:00:00
abstract：:BMPs and Hox proteins play crucial roles in developmental processes. Beyond their mutual regulation of gene expression, little is known about the relations between their mechanisms of actions. Previously, we have shown that Hoxc8 acts as a downstream repressor in the BMP signaling pathway. Smad1 and Smad6 interact wit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.002
更新日期：2006-04-01 00:00:00
abstract：:Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00218-0
更新日期：2003-08-15 00:00:00
abstract：:Various cell adhesion molecules mediate the diverse functions of the vascular endothelium, such as cell adhesion, neutrophil migration, and angiogenesis. In order to identify cell adhesion molecules important for angiogenesis, we used an in vitro model (Chalupowicz, Chowdhury, Bach, Barsigian, and Martinez, J. Cell Bi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3844
更新日期：1998-02-01 00:00:00
abstract：:The puromycin analog N6,N6-dimethyladenine (6-dimethylaminopurine or 6-DMAP) was found to inhibit meiosis reinitiation in starfish oocytes stimulated by the natural hormone 1-methyladenine. Increasing concentrations of this agent delayed and eventually blocked germinal vesicle breakdown. They were found to be effectiv...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90121-8
更新日期：1988-05-01 00:00:00
abstract：RATIONALE:Vessel formation is a crucial event in tissue repair after injury. Thus, one assumption of innovative therapeutic approaches is the understanding of its molecular mechanisms. Notwithstanding our knowledge of the role of Protein Kinase C epsilon (PKCε) in cardio-protection and vascular restenosis, its role in ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.11.011
更新日期：2015-01-15 00:00:00
abstract：:To determine whether the micronucleus makes essential contributions during asexual reproduction, observations were made on cells of Euplotes octocarinatus from which the micronucleus had been removed with a micropipette. Most cells underwent one postenucleation division, then became arrested in macronuclear G1, slowed...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90100-4
更新日期：1985-12-01 00:00:00
abstract：:Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0133
更新日期：1996-05-01 00:00:00
abstract：:Neuromedin B (NMB) is one of the bombesin-like peptides in mammals. Recently, bombesin-like peptides have been characterized as growth factors in highly vascularized tumors. In this study, we report that NMB potently stimulates in vivo neovascularization in a mouse Matrigel plug and the sprouting of endothelial cells ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.08.009
更新日期：2009-11-15 00:00:00
abstract：:It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5222
更新日期：2001-06-10 00:00:00
abstract：:Partial outlet obstruction of the rabbit bladder induces serosal thickening and smooth muscle (SM) hypertrophy. Within thickened serosa, submesothelial (mesenchymal) cells differentiate into SM cells after 30 days of obstruction[S. Buoro et al. Lab. Invest. 69, 589-602, 1993]. Here, we show that submesothelial cells t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0088
更新日期：1996-03-15 00:00:00
abstract：:The adaptor protein Shb has previously been shown to regulate apoptosis in response to cytokines and inhibitors of angiogenesis although the mechanisms governing these effects have remained obscure. We currently demonstrate interactions between Shb and c-Abl and that Shb regulates c-Abl kinase activity. The data sugge...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.10.011
更新日期：2007-01-15 00:00:00
abstract：:Integrins play a major role in cell adhesion and migration. Previous work reported that a cleaved form of integrin alpha6 (alpha6p) was detected in invasive human prostate cancer tissue, absent in normal prostate tissue and was produced by urokinase-type Plasminogen Activator (uPA) in a plasmin-independent manner. Usi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.006
更新日期：2007-04-01 00:00:00
abstract：:The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured fibroblasts and on the in vitro polymerization of microtubules were examined. MeHg caused disruption of cellular microtubules in a concentration- and time-dependent manner. Addition of the metal-chelating agent, dimercaptosuccinic acid (DMSA),...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90331-2
更新日期：1983-06-01 00:00:00
abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90088-p
更新日期：1992-10-01 00:00:00
abstract：:We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.01.003
更新日期：2007-04-15 00:00:00
abstract：:Multiple myeloma is characterized by the malignant growth of immunoglobulin producing plasma cells, predominantly in the bone marrow. The effects of primary human mesenchymal stromal cells on the differentiation phenotype of multiple myeloma cells were studied by co-culture experiments. The incubation of multiple myel...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.03.016
更新日期：2009-07-01 00:00:00
abstract：:Integrin beta 4 subunit is present in association with alpha 6 chain on both normal and transformed epithelial cells. Recently alpha 6 beta 4 heterodimer was found on the endothelium of medium-sized blood vessels and on immature thymocytes. In this report we show, by Northern blotting, indirect immunofluorescence, imm...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0271
更新日期：1996-09-15 00:00:00
abstract：:Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1148
更新日期：1995-05-01 00:00:00
abstract：:Mechanisms involved in establishing the organization and numbers of fibres in a muscle are not completely understood. During Drosophila indirect flight muscle (IFM) formation, muscle growth is achieved by both incorporating hundreds of nuclei, and hypertrophy. As a result, IFMs provide a good model with which to under...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.06.021
更新日期：2013-10-15 00:00:00
abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.03.037
更新日期：2004-07-15 00:00:00
abstract：:Studies were carried out to test the idea that transforming growth factor beta (TGFbeta) stimulated fibroblast contraction of collagen matrices by different mechanisms depending on mechanical loading on the cells and matrix. Under mechanically unloaded conditions (floating matrices), TGFbeta stimulated contraction dir...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5445
更新日期：2002-02-15 00:00:00
abstract：:Normal fibroblast subpopulations have differential surface expression of the GPI-linked raft protein Thy-1, which correlates with differences in cellular adhesion and migration in vitro. Thrombospondin-1 (TSP-1) induces an intermediate state of adhesion in fibroblasts and other cells which facilitates migration. TSP-1...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.029
更新日期：2006-11-15 00:00:00
abstract：:The nucleolar precursor bodies (NPBs) are electrondense, homogeneous, and finely filamentous intranuclear structures occurring in mammalian zygotes during the early postfertilization development and subsequently transforming into nucleoli. In this study we addressed the question if the assembly of the NPBs is correlat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90050-k
更新日期：1990-11-01 00:00:00
abstract：:In many eukaryotic organisms, initiation of DNA synthesis is associated with a major control point within the cell cycle and reflects the commitment of the cell to the DNA replication-division portion of the cell cycle. In Paramecium, the timing of DNA synthesis initiation is established prior to fission during the pr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90215-6
更新日期：1986-11-01 00:00:00
abstract：:Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.05.004
更新日期：2014-09-10 00:00:00
abstract：:Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.02.020
更新日期：2010-07-15 00:00:00
abstract：:Breast tumor cells enter the bloodstream long before the development of clinically evident metastasis. However, the early presence of such bloodborne cells predicts poor patient outcome. Nearly 90% of human breast tumors arise as carcinomas from mammary epithelial cells, so it is important to study how these cells res...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.001
更新日期：2007-04-15 00:00:00
abstract：:We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3 X 10(-8) M, ouabain also prolongs...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90449-5
更新日期：1985-05-01 00:00:00
abstract：:We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was s...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90149-5
更新日期：1990-09-01 00:00:00
