当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Cisplatin resistance in a murine leukemia cell line is associated with a defective apoptotic process.

Cisplatin resistance in a murine leukemia cell line is associated with a defective apoptotic process.

Abstract：

:Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a cisplatin-resistant cell line (L1210/DDP) derived from a L1210 murine leukemia cell line (L1210/0). Treatments of the parental L1210/0 cell line with two DNA damaging agents (cisplatin and 5-azacytidine) or a protein kinase C inhibitor (staurosporine) led to biochemical events characteristic of apoptosis (as determined by the cell morphology and the oligonucleosomal DNA fragmentation). In contrast, the cisplatin-resistant L1210/DDP subline, which was cross-resistant to 5-azacytidine, did not exhibit any DNA fragmentation or morphological changes typical of apoptosis when exposed to toxic concentrations of either cisplatin or 5-azacytidine. The failure of these cells to undergo apoptosis upon cisplatin or 5-azacytidine exposure has been correlated with the lack of a nuclear endonuclease activity present in wild-type cell nuclei. However, staurosporine, which exerted the same toxicity on both cell lines, induced the internucleosomal DNA fragmentation and morphological features of apoptosis in both of them. This indicates that a functional pathway for apoptosis is preserved in the resistant cells. The induction of this pathway can be correlated with the presence of a cytoplasmic endonuclease activity whose specificity seems different from that operating in L1210/0 cells in terms of cation and pH dependence. Therefore, in these cell lines, different endonucleases are possibly involved in apoptosis. In response to treatment with drugs having different targets, the apoptotic cell death may operate through different signaling pathways, one of them being possibly defective in the L1210/DDP-resistant cells.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Segal-Bendirdjian E,Jacquemin-Sablon A

doi

10.1006/excr.1995.1148

subject

Has Abstract

pub_date

1995-05-01 00:00:00

pages

201-12

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(85)71148-2

journal_volume

218

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Modification of integrin-mediated cell attachment to substrata by serine proteinases in the presence and absence of divalent cations.

    abstract：:The sensitivity to serine proteinases of cellular proteins involved in cell-matrix adhesion was investigated using C32 melanoma cells. Cells dissociated from monolayers by the metal chelator ethylenediaminetetraacetic acid were incubated with proteolytic enzymes, and then attachment was quantified by standard cell adh...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1226

    authors： Fujii K,Lazarus GS,Schechter NM

    更新日期：1993-09-01 00:00:00

  • The expression and activity of D-type cyclins in F9 embryonal carcinoma cells: modulation of growth by RXR-selective retinoids.

    abstract：:The growth rate of malignant F9 embryonal carcinoma cells slows considerably following all-trans-retinoic acid-induced differentiation into benign parietal endoderm. To determine the mechanism of this process, we examined the expression of cyclins D1, D2, and D3 and the activity of their associated kinases. Cyclin D1 ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4664

    authors： Li Y,Glozak MA,Smith SM,Rogers MB

    更新日期：1999-12-15 00:00:00

  • Thy-1, via its GPI anchor, modulates Src family kinase and focal adhesion kinase phosphorylation and subcellular localization, and fibroblast migration, in response to thrombospondin-1/hep I.

    abstract：:Normal fibroblast subpopulations have differential surface expression of the GPI-linked raft protein Thy-1, which correlates with differences in cellular adhesion and migration in vitro. Thrombospondin-1 (TSP-1) induces an intermediate state of adhesion in fibroblasts and other cells which facilitates migration. TSP-1...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.07.029

    authors： Rege TA,Pallero MA,Gomez C,Grenett HE,Murphy-Ullrich JE,Hagood JS

    更新日期：2006-11-15 00:00:00

  • An altered repertoire of fos/jun (AP-1) at the onset of replicative senescence.

    abstract：:With multiple divisions in culture, normal diploid cells suffer a loss of growth potential that leads to replicative senescence and a finite replicative capacity. Using quantitative RT-PCR, we have monitored mRNA expression levels of c-fos, c-jun, JunB, c-myc, p53, H-ras, and histone H4 during the replicative senescen...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90415-5

    authors： Irving J,Feng J,Wistrom C,Pikaart M,Villeponteau B

    更新日期：1992-09-01 00:00:00

  • Cyclosporin A is a differential inhibitor of eukaryotic RNA polymerases.

    abstract：:In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90382-3

    authors： Brack C,Mattaj IW,Gautschi J,Cammisuli S

    更新日期：1984-04-01 00:00:00

  • Residues in the alternative reading frame tumor suppressor that influence its stability and p53-independent activities.

    abstract：:The Alternative Reading Frame (ARF) protein suppresses tumorigenesis through p53-dependent and p53-independent pathways. Most of ARF's anti-proliferative activity is conferred by sequences in its first exon. Previous work showed specific amino acid changes occurred in that region during primate evolution, so we progra...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.01.010

    authors： di Tommaso A,Hagen J,Tompkins V,Muniz V,Dudakovic A,Kitzis A,Ladeveze V,Quelle DE

    更新日期：2009-04-15 00:00:00

  • Effects of antiandrogens on chromatin remodeling and transcription of the integrated mouse mammary tumor virus promoter.

    abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5018

    authors： List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

    更新日期：2000-10-10 00:00:00

  • Coordination of growth and differentiation in the fetal lung.

    abstract：:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90281-e

    authors： Nielsen HC,Kirk WO,Sweezey N,Torday JS

    更新日期：1990-05-01 00:00:00

  • Cytokeratin 19 regulates endoplasmic reticulum stress and inhibits ERp29 expression via p38 MAPK/XBP-1 signaling in breast cancer cells.

    abstract：:Cytokeratin 19 (CK19) is widely used as a biomarker for the detection of disseminated tumor cells in blood and bone marrow, and its positivity is considered as an independent prognostication indicator in cancer patients. However, its role in breast cancer progression remains unknown. We had established a stable CK19-e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.02.017

    authors： Bambang IF,Lu D,Li H,Chiu LL,Lau QC,Koay E,Zhang D

    更新日期：2009-07-01 00:00:00

  • Embryonic lineage of vascular smooth muscle cells determines responses to collagen matrices and integrin receptor expression.

    abstract：:Developmental studies have demonstrated that the vascular smooth muscle cells (VSMC) present within the elastic arteries are differentiated from two definitive origins, the neural crest and the mesoderm. Cells from these distinct progenitors differ in their ability to determine long-range spatial order of the extracel...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0258

    authors： Thieszen SL,Dalton M,Gadson PF,Patterson E,Rosenquist TH

    更新日期：1996-08-25 00:00:00

  • High yields of specific hybridomas obtained by electrofusion of murine lymphocytes immunized in vivo or in vitro.

    abstract：:We have studied the use of electrofusion to obtain hybridomas producing antigen-specific antibodies after immunization of murine lymphocytes in vitro. Under optimal conditions fusion frequencies of the order of magnitude of 10(-3) were obtained, which is approximately 80-fold higher than the mean value obtained with f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90405-9

    authors： van Duijn G,Langedijk JP,de Boer M,Tager JM

    更新日期：1989-08-01 00:00:00

  • Osmotic swelling of endocytic compartments induced by internalized sucrose is restricted to mature lysosomes in cultured mammalian cells.

    abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90156-o

    authors： DeCourcy K,Storrie B

    更新日期：1991-01-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.08.010

    authors： Zhang J,Harrison JS,Studzinski GP

    更新日期：2011-01-01 00:00:00

  • Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans.

    abstract：:The mammalian Hippo pathway comprises mammalian Ste20-like kinases (MST1/2) and large tumor suppressor kinases (LATS1/2). LATS1/2, which are activated by MST1/2, phosphorylate a transcriptional co-activator, yes-associated protein (YAP), and induce the recruitment of YAP by 14-3-3 to cytoplasm, so that the TEAD-depend...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.01.020

    authors： Iwasa H,Maimaiti S,Kuroyanagi H,Kawano S,Inami K,Timalsina S,Ikeda M,Nakagawa K,Hata Y

    更新日期：2013-04-15 00:00:00

  • The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts.

    abstract：:Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fiber...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.013

    authors： Liu Z,Ho CH,Grinnell F

    更新日期：2014-08-15 00:00:00

  • p53-independent upregulation of KILLER/DR5 TRAIL receptor expression by glucocorticoids and interferon-gamma.

    abstract：:KILLER/DR5 is a death-domain-containing proapoptotic receptor that binds to the cytotoxic ligand TRAIL. It was originally reported that induction of KILLER/DR5 mRNA following DNA damage was p53-dependent, but some drugs that induce apoptosis can upregulate KILLER/DR5 mRNA expression in cell lines with mutated p53. We ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5073

    authors： Meng RD,El-Deiry WS

    更新日期：2001-01-15 00:00:00

  • Immortalization of primary cells by DNA tumor viruses.

    abstract：:Cellular senescence is characterized by a decline in sensitivity to growth factors resulting in cessation of cellular growth. The expression of cellular or viral oncogenes may result in the establishment of cell lines with unlimited proliferative potential ("immortalization"). A variety of viral and cellular oncogenes...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/0014-4827(90)90027-8

    authors： Linder S,Marshall H

    更新日期：1990-11-01 00:00:00

  • Thrombin-mast cell interactions. Binding and cell activation.

    abstract：:Activation of mouse bone marrow-derived mast cells (BMMC) by thrombin (0.05-0.5 U/million cells) resulted in a concentration-dependent release of histamine, which levelled off by 0.1 U thrombin. Rat peritoneal mast cells (RMC) were not stimulated by thrombin, though in control experiments, both types of mast cells deg...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90184-3

    authors： Razin E,Baranes D,Marx G

    更新日期：1985-10-01 00:00:00

  • Vectorial transcytosis of immunoglobulin G by human term trophoblast cells in culture.

    abstract：:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90154-m

    authors： Sooranna SR,Contractor SF

    更新日期：1991-01-01 00:00:00

  • Cellular localization and expression of template-activating factor I in different cell types.

    abstract：:Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3930

    authors： Nagata K,Saito S,Okuwaki M,Kawase H,Furuya A,Kusano A,Hanai N,Okuda A,Kikuchi A

    更新日期：1998-05-01 00:00:00

  • Screening of genes involved in cell migration in Dictyostelium.

    abstract：:A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.12.002

    authors： Nagasaki A,Uyeda TQ

    更新日期：2008-03-10 00:00:00

  • Senescence stimulates U937-endothelial cell interactions.

    abstract：:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1246

    authors： Maier JA,Statuto M,Ragnotti G

    更新日期：1993-09-01 00:00:00

  • ERα inhibited myocardin-induced differentiation in uterine fibroids.

    abstract：:Uterine fibroids, also known as uterine leiomyomas, are a benign tumor of the human uterus and the commonest estrogen-dependent benign tumor found in women. Myocardin is an important transcriptional regulator in smooth and cardiac muscle development. The role of myocardin and its relationship with ERα in uterine fibro...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.11.007

    authors： Liao XH,Li JY,Dong XM,Wang X,Xiang Y,Li H,Yu CX,Li JP,Yuan BY,Zhou J,Zhang TC

    更新日期：2017-01-01 00:00:00

  • Accumulation of autolysosomes after receptor-mediated introduction of Ep459-asialofetuin conjugate into lysosomes in rat hepatocytes.

    abstract：:Administration of Ep459-asialofetuin conjugate (Ep459-AF) and pepstatin-asialofetuin conjugate (Ps-AF) to rats effectively inhibited lysosomal BANA hydrolase and cathepsin D in the liver, respectively, at a very low dose. Ep459-AF treatment also led to an accumulation of autolysosomes in rat liver. There was a close c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90257-5

    authors： Furuno K,Miwa N,Ishikawa T,Kato K

    更新日期：1985-01-01 00:00:00

  • The role of alpha3beta1 integrin in determining the supramolecular organization of laminin-5 in the extracellular matrix of keratinocytes.

    abstract：:Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(02)00028-9

    authors： deHart GW,Healy KE,Jones JC

    更新日期：2003-02-01 00:00:00

  • Regulation of subcellular localization of the antiproliferative protein Tob by its nuclear export signal and bipartite nuclear localization signal sequences.

    abstract：:Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.12.016

    authors： Maekawa M,Yamamoto T,Nishida E

    更新日期：2004-04-15 00:00:00

  • Myristoylation is required for the intracellular localization and endocytic function of ARF6.

    abstract：:ADP-ribosylation factors (ARFs) are modified by a myristate group that is covalently linked to the second glycine residue at the amino terminus. With the recent evidence that ARF6 localizes to the cell periphery and plays a regulatory role in endocytic traffic, we have investigated the role of myristoylation on the me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1362

    authors： D'Souza-Schorey C,Stahl PD

    更新日期：1995-11-01 00:00:00

  • The trafficking and regulation of membrane receptors by the RING-CH ubiquitin E3 ligases.

    abstract：:Ubiquitylation of membrane receptors is recognised as a critical post-translational modification, governing their regulation and function. Following ubiquitylation, membrane proteins may be internalised, recycled or degraded via lysosomal or proteasomal pathways. Viruses have appropriated these cellular pathways as a ...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2008.10.026

    authors： Nathan JA,Lehner PJ

    更新日期：2009-05-15 00:00:00

  • External Ca2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

    abstract：:Polycystin-2 (PC2, TRPP2) is a nonselective cation channel whose dysfunction is associated with the onset of autosomal dominant polycystic kidney disease (ADPKD). PC2 contributes to Ca2+ transport and cell signaling in renal epithelia and other tissues. Little is known however, as to the external Ca2+ regulation of PC...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.11.004

    authors： Dai XQ,Perez PL,Soria G,Scarinci N,Smoler M,Morsucci DC,Suzuki K,Cantero MDR,Cantiello HF

    更新日期：2017-01-01 00:00:00

  • Inhibition of the melanoma cell cycle and regulation at the G1/S transition by 12-O-tetradecanoylphorbol-13-acetate (TPA) by modulation of CDK2 activity.

    abstract：:The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1356

    authors： Coppock DL,Buffolino P,Kopman C,Nathanson L

    更新日期：1995-11-01 00:00:00