Abstract:
:We have studied the use of electrofusion to obtain hybridomas producing antigen-specific antibodies after immunization of murine lymphocytes in vitro. Under optimal conditions fusion frequencies of the order of magnitude of 10(-3) were obtained, which is approximately 80-fold higher than the mean value obtained with fusion induced by polyethylene glycol. The number of antigen-specific hybridomas was also increased in a comparable way. The high yields of specific hybridomas observed with electrofusion were independent of the immunization procedure, the antigen or the hapten of interest, or the sources of the lymphocytes. The data presented in this paper indicate that electrofusion may be an extremely attractive alternative method for immortalization of human lymphocytes following immunization in vitro.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
van Duijn G,Langedijk JP,de Boer M,Tager JMdoi
10.1016/0014-4827(89)90405-9subject
Has Abstractpub_date
1989-08-01 00:00:00pages
463-72issue
2eissn
0014-4827issn
1090-2422journal_volume
183pub_type
杂志文章abstract::The influence of the cylindrical substratum surface on the actin microfilament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0354
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abstract::We had identified earlier a germ cell-specific lamin of 60 kDa in rat which is related to somatic lamin B. This polypeptide was shown to be the only major component organizing the lamina structure of round spermatids. In the present study, we find that this 60-kDa polypeptide persists in the testicular and epididymal ...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(92)90151-w
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90167-2
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abstract::A novel fluorescence-based method was developed to measure HIV envelope glycoprotein (env)-CD4-mediated cell fusion. This method measures the spread of a fluorescent dye as the cytosolic compartments of adjacent cells become contiguous upon cell-to-cell fusion. Calcein-labeled CD4+ Sup-T1 cells were seeded onto a mono...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.3939
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.3364
更新日期:1997-01-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
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更新日期:2004-07-01 00:00:00
abstract::Rafoxanide is commonly used as anti-helminthic medicine in veterinary medicine, a main compound of salicylanilide. Previous studies have reported that rafoxanide, as an inhibitor of BRAF V600E mutant protein, inhibits the growth of colorectal cancer, multiple myeloma, and skin cancer. However, its therapeutic effect o...
journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2019-12-15 00:00:00
abstract::The process of DNA synthesis in normal and endoreduplicating mammalian cells are very similar. Both types of chromosomes are replicated in defined units termed chromosomal replicons, and in the same sequences along their lengths. The sister chromosomes of the diplochromosomes are replicated synchronously in identical ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90148-9
更新日期:1983-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90192-9
更新日期:1988-04-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.12.021
更新日期:2004-04-15 00:00:00
abstract::We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0098
更新日期:1996-03-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.02.016
更新日期:2007-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5073
更新日期:2001-01-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.06.018
更新日期:2017-09-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90318-2
更新日期:1989-12-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.02.011
更新日期:2017-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.03.054
更新日期:2017-06-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90531-8
更新日期:1986-07-01 00:00:00
abstract::T51B rat liver epithelial cells were stimulated with extracellular ATP. Changes in cytoplasmic free Ca2+ concentration [( Ca2+]i) were measured by fura-2 both in a large population of cells on coverslips in a cuvette and in single cells in a microscopic system. Extracellular ATP evoked a prompt increase in [Ca2+]i in ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90198-5
更新日期:1989-03-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5540
更新日期:2002-07-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1177
更新日期:1994-07-01 00:00:00
abstract::The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1073
更新日期:1995-03-01 00:00:00
abstract::In confluent keratinocyte monolayers, desmosomal adhesion gradually becomes calcium-independent and this is associated with an increase in the strength of intercellular adhesion (hyper-adhesion). In this study, we investigated the functional and molecular significance of hyper-adhesion in a system challenged by autoim...
journal_title:Experimental cell research
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更新日期:2010-02-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.06.005
更新日期:2008-08-15 00:00:00
abstract::The double-stranded RNA-dependent kinase, PKR, is encoded by an interferon inducible gene and is largely responsible for the anti-viral effects of this cytokine. Recent studies have shown that PKR may also play a role in the regulation of normal cellular growth. Although numerous examples of viral strategies for inact...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4201
更新日期:1998-11-01 00:00:00