Abstract：
:In order to investigate the molecular mechanism of biological action of the drug cyclosporin A (CsA), we have studied its effect on eukaryotic RNA polymerase activities in two systems: (a) in vitro transcription; (b) injection into frog oocytes. The electron microscopic transcription R-loop method [1] has been used to assay for specific transcription of an immunoglobulin gene clone in HeLa cell lysates. In this system, CsA inhibits efficiently the transcription of immunoglobulin genes. Dilution experiments show that RNA polymerase II activity is inhibited by nanomolar concentrations (i.e. amounts comparable to the ones needed for the inhibition of lymphocyte stimulation), whereas polymerase III is inhibited at micromolar concentrations. In vitro transcription with E. coli RNA polymerase is not inhibited. Parallel experiments with the mushroom toxin alpha-amanitin have shown that the eukaryotic RNA polymerase II displays similar sensitivity towards the two cyclic peptides CsA and alpha-amanitin in vitro. When CsA is injected into frog oocytes, together with a cloned Xenopus laevis U1 small nuclear RNA gene, transcription of this gene by endogenous RNA polymerase II is inhibited by 60%. RNA polymerase III and RNA polymerase I transcription is not inhibited in the oocyte. Various possibilities for the selective action of CsA on stimulated lymphocytes are discussed.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Brack C,Mattaj IW,Gautschi J,Cammisuli Sdoi
10.1016/0014-4827(84)90382-3subject
Has Abstractpub_date
1984-04-01 00:00:00pages
314-21issue
2eissn
0014-4827issn
1090-2422pii
0014-4827(84)90382-3journal_volume
151pub_type
杂志文章abstract：:Seven continuous cell lines have been established in vitro from lethal embryos produced by the female sterile mutant mh 1182 of Drosophila melanogaster. Six lines show haploid metaphases. Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90371-9
更新日期：1984-03-01 00:00:00
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doi：10.1016/0014-4827(86)90555-0
更新日期：1986-03-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1006/excr.1994.1155
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abstract：:Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...
journal_title：Experimental cell research
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abstract：:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...
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pub_type： 杂志文章
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更新日期：2003-09-10 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.11.021
更新日期：2009-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90116-4
更新日期：1988-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90013-x
更新日期：1992-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.10.009
更新日期：2016-06-10 00:00:00
abstract：:The duplication and segregation of the genome during cell division is crucial to maintain cell identity, development of organisms and tissue maintenance. Centromeres are at the basis of accurate chromosome segregation as they define the site of assembly of the kinetochore, a large complex of proteins that attaches to ...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2020-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.02.028
更新日期：2014-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90106-7
更新日期：1989-04-01 00:00:00
abstract：:Nucleoside diphosphate (NDP) kinases, responsible for the synthesis of nucleoside triphosphates and produced by the nm23 genes, are involved in numerous regulatory processes associated with proliferation, development, and differentiation. Their possible role in providing the GTP/ATP required for sperm function is unkn...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00268-4
更新日期：2003-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3799
更新日期：1997-12-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.10.021
更新日期：2010-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90318-2
更新日期：1989-12-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1983-08-01 00:00:00
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journal_title：Experimental cell research
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更新日期：2017-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1311
更新日期：1995-09-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1988-12-01 00:00:00
abstract：:Skeletal muscle-derived CD34+/45- (Sk-34) cells were identified as a new candidate for stem cells. However, the relationship between Sk-34 cells and side-population (SP) cells is unknown. Here, we demonstrate that Sk-34 cells prepared from murine skeletal muscles consist wholly of main-population (MP) cells. The Sk-34...
journal_title：Experimental cell research
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doi：10.1016/s0014-4827(03)00376-8
更新日期：2003-11-15 00:00:00
abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2006.04.007
更新日期：2006-08-01 00:00:00
abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.06.020
更新日期：2005-10-01 00:00:00
abstract：:We investigated whether cell-permeable, synthetic ceramide (C6 ceramide) could induce apoptosis in Fas-resistant Hodgkin's disease (HD)-derived cell lines. Despite strongly expressing the Fas-receptor, two of three HD-derived cell lines were resistant to Fas-mediated apoptosis. This resistance to Fas could not be attr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4773
更新日期：2000-02-25 00:00:00
