Abstract:
:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung protein synthesis relative to DNA synthesis peaked earlier in gestation in the female fetal lung and that this event was synchronous with the onset of differentiation. Pregnant rats were treated with dihydrotestosterone (DHT) during pregnancy, and fetal lung growth parameters were measured. Lung wet weight, dry weight, and DNA and protein concentrations were significantly elevated by DHT treatment. Type II cells and fibroblasts were isolated from lungs of DHT-treated fetuses. The number of total cells recovered was increased by 30%; the number of type II cells recovered was increased by 87%; and the number of fibroblasts recovered was increased by 42%. The type II cells which were recovered exhibited increased incorporation of [3H]thymidine into DNA and a reduced ratio of radiolabeled protein to radiolabeled DNA compared to that of cells from control lungs. Further studies were done in vitro with fibroblasts and type II cells isolated from untreated fetal rat lungs. Treatment of the fibroblasts with DHT during culture caused an increase in thymidine incorporation into DNA. This effect was not blocked by simultaneous treatment with cortisol, which normally causes reduced DNA synthesis and induces fibroblast differentiation. Treatment of the type II cells with DHT in culture caused a dose-dependent increase in cell number but a decrease in synthesis of disaturated phosphatidylcholine. These studies provide more direct evidence of the interrelationships between the control of growth and the control of differentiation in the fetal lung. DHT, a signal which delays the onset of expression of differentiation, also induces growth. We conclude that the controls of growth and of differentiation of the fetal lung are reciprocally linked.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Nielsen HC,Kirk WO,Sweezey N,Torday JSdoi
10.1016/0014-4827(90)90281-esubject
Has Abstractpub_date
1990-05-01 00:00:00pages
89-96issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(90)90281-Ejournal_volume
188pub_type
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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pub_type: 杂志文章
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90173-x
更新日期:1987-08-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1095
更新日期:1994-04-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90174-5
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journal_title:Experimental cell research
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更新日期:2011-05-15 00:00:00
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journal_title:Experimental cell research
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doi:10.1006/excr.2000.5055
更新日期:2000-12-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00