Abstract:
:Different extracellular matrix (ECM) molecules, when presented to hippocampal neurons in culture in a substrate-bound form, exert strikingly similar effects on the establishment of neuronal polarity, i.e., the growth of axon-like major neurites is favored, whereas extension of dendrite-like minor neurites is inhibited. To gain insight into the underlying signal transduction processes, we have investigated the effects of modulators of protein kinase activity on the morphology of neurons cultured on tenascin-R, tenascin-C, and laminin-entactin substrates. We found differential effects of broad-spectrum protein kinase inhibitors: H-7 promoted the growth of minor neurites, whereas H-8 reduced the growth of major neurites on ECM but not control substrates. In contrast, chelerythrine, a specific inhibitor of protein kinase C, selectively affected growth of both minor and major neurites on control, but not on ECM substrates. Finally, reagents which elevate intracellular cAMP levels facilitated growth of minor neurites and inhibited growth of major neurites and thus interfered with the establishment of a polarized phenotype on both ECM and control substrates. Our results suggest that protein kinases mediate the effects of ECM molecules on neuronal polarity and that different kinases control extension of axons and dendrites.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Lochter A,Schachner Mdoi
10.1006/excr.1997.3655subject
Has Abstractpub_date
1997-08-25 00:00:00pages
124-9issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(97)93655-7journal_volume
235pub_type
杂志文章abstract::In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4b...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.03.010
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journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2007.07.004
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abstract::Advanced stage neuroblastomas (NB) exhibit a tissue inhibitor of metalloproteinase (TIMP)-2/matrix metalloproteinase (MMP) imbalance, considered a prerequisite for MMP involvement in tumor progression in vivo. Human SH-SY5Y NB cells exhibit a similar TIMP-2/MMP imbalance that promotes in vitro invasive behavior that i...
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3799
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
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更新日期:2016-04-10 00:00:00
abstract::Based on data from developmental RNA profiles and in situ hybridization, we report a direct examination of the expression of one collagen gene (Dcg1) during drosophila melanogaster life cycle. These studies show, for the first time, that the expression of a collagen gene is both differential and tissue-specific during...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90071-6
更新日期:1986-04-01 00:00:00
abstract::Skeletal muscle-derived CD34+/45- (Sk-34) cells were identified as a new candidate for stem cells. However, the relationship between Sk-34 cells and side-population (SP) cells is unknown. Here, we demonstrate that Sk-34 cells prepared from murine skeletal muscles consist wholly of main-population (MP) cells. The Sk-34...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00376-8
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abstract::We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1193
更新日期:1993-08-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has led to kinds of clinical disorders and great morbidity worldwide, such as sensorineural hearing loss (SNHL), mental retardation, and developmental delays in immunocompromised individuals. Congenital HCMV infection is a leading cause of birth defects, primarily manifesting as neurologic...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.111746
更新日期:2020-01-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.2000.5039
更新日期:2000-11-25 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.11.036
更新日期:2018-01-15 00:00:00
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journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1281
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abstract::Ring finger protein 6 (RNF6), a RING-domain E3 ubiquitin Ligase, functions as a potential tumor promoter in several caners. However, the function and clinical significance of RNF6 in hepatocellular carcinoma (HCC) remains unclear. Here, we found that high RNF6 expression was detected frequently in primary HCC tissues,...
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.06.021
更新日期:2009-10-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90099-v
更新日期:1990-04-01 00:00:00
abstract::The role of protein kinase C (PKC) during fertilization in the model eukaryote Dictyostelium discoideum was studied. Inhibition of PKC activity using staurosporine, chelerythrine, and bisindoylmaleimide resulted in a dose-dependent decrease in gamete fusion without any detectable effect on cell morphology or growth. A...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1322
更新日期:1995-10-01 00:00:00
abstract::X-ray-induced damage leads to cell-cycle "checkpoint" arrest by p53-dependent induction of the cyclin-dependent kinase inhibitor p21 (Waf1/Cip1/Sdi1). Human tumor cells that lack this response fail to arrest after exposure to DNA-damaging agents, undergo multiple rounds of endoreduplicative DNA synthesis, and eventual...
journal_title:Experimental cell research
pub_type: 杂志文章
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abstract::The p53-regulated stress-inducible gene GADD45 has been shown to participate in cellular response to DNA damage, including cell cycle checkpoint, apoptosis, and DNA repair. However, the regulation of GADD45 expression is complex and may involve both p53-dependent and -independent pathways. Recent findings have demonst...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5312
更新日期:2001-09-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90253-q
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