Control of apoptosis and growth of malignant T lymphoma cells by lymph node stromal cells.


:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underwent apoptosis when separated from the stromal CA-12 cells. Apoptosis of CS-21 lymphoma cells was determined by observation of morphological changes using a transmission electron microscope and also by detection of nuclear DNA degradative fragments of oligonucleosomal size. Stromal CA-12 cells secreted soluble factors that enhanced DNA synthesis in CS-21 lymphoma cells. The soluble factors, however, were not sufficient to prevent apoptosis of CS-21 cells. Apoptosis of CS-21 lymphoma cells was suppressed only when the CS-21 lymphoma cells were cocultured with substantial numbers of CA-12 cells. The results suggest that the lymph node stromal CA-12 cells played an important role in the growth of CS-21 lymphoma cells by providing at least two different signals. One signal prevented CS-21 cells from apoptotic cell death by direct cell-to-cell contact, and the other signal enhanced the CS-21 cell proliferation by secreted soluble factors.


Exp Cell Res


Kataoka S,Naito M,Fujita N,Ishii H,Ishii S,Yamori T,Nakajima M,Tsuruo T




Has Abstract


1993-08-01 00:00:00














  • Augmenter of liver regeneration inhibits TGF-β1-induced renal tubular epithelial-to-mesenchymal transition via suppressing TβR II expression in vitro.

    abstract::Tubular epithelial-to-mesenchymal transition (EMT) plays a crucial role in the progression of renal tubular interstitial fibrosis (TIF), which subsequently leads to chronic kidney disease (CKD) and eventually, end-stage renal disease (ESRD). We propose that augmenter of liver regeneration (ALR), a member of the newly ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liao XH,Zhang L,Chen GT,Yan RY,Sun H,Guo H,Liu Q

    更新日期:2014-10-01 00:00:00

  • Exchange of chemical signals between cardiac cells. Fundamental role on cell communication and metabolic cooperation.

    abstract::The exchange of chemical signals between cardiac cells and its relevance for cell communication and metabolic cooperation was reviewed. The role of gap junctions on the transfer of chemical information was discussed as well as the different factors involved in its regulation including changes in cell volume, high gluc...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: De Mello WC

    更新日期:2016-08-01 00:00:00

  • Analysis of Tax-expressing cell lines generated from HTLV-I tax-transgenic mice: correlation between c-myc overexpression and neoplastic potential.

    abstract::Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saggioro D,D'agostino DM,Chieco-Bianchi L

    更新日期:1999-03-15 00:00:00

  • The glomerular basement membrane.

    abstract::The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Miner JH

    更新日期:2012-05-15 00:00:00

  • Protein phosphatase 2A is involved in the regulation of protein kinase A signaling pathway during in vitro chondrogenesis.

    abstract::We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zákány R,Szucs K,Bakó E,Felszeghy S,Czifra G,Bíró T,Módis L,Gergely P

    更新日期:2002-04-15 00:00:00

  • Factor Xa stimulates fibroblast procollagen production, proliferation, and calcium signaling via PAR1 activation.

    abstract::Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR1)....

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Blanc-Brude OP,Archer F,Leoni P,Derian C,Bolsover S,Laurent GJ,Chambers RC

    更新日期:2005-03-10 00:00:00

  • Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells.

    abstract::We report here the first direct observation of chemotaxis to EGF by rat mammary carcinoma cells. When exposed to a gradient of EGF diffusing from a micropipette, MTLn3 cells displayed typical ameboid chemotaxis, extending a lamellipod-like protrusion and moving toward the pipette. Using a homogeneous upshift in EGF to...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bailly M,Yan L,Whitesides GM,Condeelis JS,Segall JE

    更新日期:1998-06-15 00:00:00

  • Human autoantibody to topoisomerase II.

    abstract::The rheumatic diseases are characterized by the production of autoantibodies that are usually directed against components of the cell nucleus. In this communication, we describe autoantibodies that recognize DNA topoisomerase II (anti-topoII) present in the serum of a patient with systemic lupus erythematosus. Several...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hoffmann A,Heck MM,Bordwell BJ,Rothfield NF,Earnshaw WC

    更新日期:1989-02-01 00:00:00

  • Akt phosphorylation and NFkappaB activation are counterregulated under conditions of oxidative stress.

    abstract::This study was designed to elucidate the mechanisms involved in elevated cell death arising from an altered endogenous oxidant state. Increased levels of cell death were detected in cells lacking Gpx1 following the addition of exogenous H2O2. This increased apoptosis correlated with a down-regulation in the activation...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Taylor JM,Crack PJ,Gould JA,Ali U,Hertzog PJ,Iannello RC

    更新日期:2004-11-01 00:00:00

  • Involvement of LPA signaling via LPA receptor-2 in the promotion of malignant properties in osteosarcoma cells.

    abstract::Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors mediates various biological effects in cancer cells. This study aimed to investigate the roles of LPA receptors in the regulation of cellular functions during tumor progression in osteosarcoma cells. Long-term cisplatin (CDDP)-treated MG63-C and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Takahashi K,Fukushima K,Tanaka K,Minami K,Ishimoto K,Otagaki S,Fukushima N,Honoki K,Tsujiuchi T

    更新日期:2018-08-15 00:00:00

  • Macrophages enhance tumor-derived autophagosomes (DRibbles)-induced B cells activation by TLR4/MyD88 and CD40/CD40L.

    abstract::Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

    更新日期:2015-02-15 00:00:00

  • The nucleoside diphosphate kinase activity of DRnm23 is not required for inhibition of differentiation and induction of apoptosis in 32Dcl3 myeloid precursor cells.

    abstract::DRnm23 belongs to a multigene family which includes nm23-H1, the first bona fide metastasis suppressor gene, nm23-H2, nm23-H4, and nm23-H5. Like nm23-H1, nm23-H2, and nm23-H4, DRnm23 possesses nucleoside diphosphate kinase (NDPK) activity. Upon overexpression in myeloid precursor 32Dcl3 cells, DRnm23 inhibits granuloc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Venturelli D,Cesi V,Ransac S,Engelhard A,Perrotti D,Calabretta B

    更新日期:2000-06-15 00:00:00

  • Permeability and metabolic properties of a trophoblast cell line (HRP-1) derived from normal rat placenta.

    abstract::The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophob...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shi F,Soares MJ,Avery M,Liu F,Zhang X,Audus KL

    更新日期:1997-07-10 00:00:00

  • Co-localization of the retinoblastoma protein and the Epstein-Barr virus-encoded nuclear antigen EBNA-5.

    abstract::A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jiang WQ,Szekely L,Wendel-Hansen V,Ringertz N,Klein G,Rosén A

    更新日期:1991-12-01 00:00:00

  • Specific activation of a STAT family member in Xiphophorus melanoma cells.

    abstract::Melanoma formation in the teleost fish Xiphophorus is caused by the uncontrolled activity of the genetically defined tumor locus Tu. The critical component of this locus is the Xmrk oncogene encoding a subclass I receptor tyrosine kinase. Overexpression and constitutive activation of the Xmrk receptor triggers a set o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Baudler M,Schartl M,Altschmied J

    更新日期:1999-06-15 00:00:00

  • Construction of transferrin-coated liposomes for in vivo transport of exogenous DNA to bone marrow erythroblasts in rabbits.

    abstract::A method for coating liposomes with transferrin is described. Such liposomes have been used for carrying exogenous DNA to rabbit bone marrow (BM) precursor cells, in vivo. A large amount of the DNA contained in the liposomes was delivered specifically into the erythroblasts of the test animals. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Stavridis JC,Deliconstantinos G,Psallidopoulos MC,Armenakas NA,Hadjiminas DJ,Hadjiminas J

    更新日期:1986-06-01 00:00:00

  • p53 Induces myocyte apoptosis via the activation of the renin-angiotensin system.

    abstract::The mechanism by which p53 activates apoptosis in various cell systems is unknown. In the absence of an external death stimulus, p53 and p53-dependent genes, bcl-2 and bax, cannot trigger apoptosis. However, p53 may enhance not only transcription of bax and repress bcl-2, but also may upregulate the local renin-angiot...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pierzchalski P,Reiss K,Cheng W,Cirielli C,Kajstura J,Nitahara JA,Rizk M,Capogrossi MC,Anversa P

    更新日期:1997-07-10 00:00:00

  • Genetic analysis of macromolecular transport across the nuclear envelope.

    abstract::Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Corbett A,Ferrigno P,Henry M,Kahana J,Koepp D,Lee M,Nguyen L,Schlenstedt G,Seedorf M,Shen E,Taura T,Wong D,Silver P

    更新日期:1996-12-15 00:00:00

  • Differential expression of Raf-1 protooncogene in resting and activated human leukocyte populations.

    abstract::In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Colotta F,Polentarutti N,Mantovani A

    更新日期:1991-06-01 00:00:00

  • Membrane glycoproteins involved in hepatocyte adhesion to collagen type I.

    abstract::Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gullberg D,Terracio L,Rubin K

    更新日期:1988-04-01 00:00:00

  • R5, a neutralizing antibody to Robo1, suppresses breast cancer growth and metastasis by inhibiting angiogenesis via down-regulating filamin A.

    abstract::Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

    更新日期:2020-02-01 00:00:00

  • Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells.

    abstract::3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tamgüney T,Zhang C,Fiedler D,Shokat K,Stokoe D

    更新日期:2008-07-01 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract::On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ohno T,Kako R,Sato C,Ohkawa A

    更新日期:1986-04-01 00:00:00

  • Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.

    abstract::Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

    更新日期:2010-07-15 00:00:00

  • Poly(ADP-ribose) polymerases in double-strand break repair: focus on PARP1, PARP2 and PARP3.

    abstract::Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage respon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Beck C,Robert I,Reina-San-Martin B,Schreiber V,Dantzer F

    更新日期:2014-11-15 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract::A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moore SA

    更新日期:1987-08-01 00:00:00

  • Compared intracellular localization of the glucocorticosteroid and progesterone receptors: an immunocytochemical study.

    abstract::The intracellular distribution of the glucocorticosteroid and progesterone receptors (GR and PR, respectively) was studied immunohistochemically. In control adrenalectomized (Adx) rat liver, immunostaining of paraffin sections revealed GR in cell nuclei, with a wide range of intensity between individuals. Following de...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gasc JM,Delahaye F,Baulieu EE

    更新日期:1989-04-01 00:00:00

  • Establishment and characterization of hybrid rat mast cells.

    abstract::Rat peritoneal mast cells (RPMC) and rat basophilic leukemia (RBL) cells are representative of connective tissue-type (CTMC) and mucosal-type (MMC) mast cells, respectively. Using polyethylene glycol, we have fused RPMC with 6-thioguanine resistant, HAT (hypoxanthine, aminopterin, thymidine) sensitive RBL-CA10.7 or RB...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zheng YL,Chan BM,Rector ES,Berczi I,Froese A

    更新日期:1991-06-01 00:00:00

  • Collagen XIV and a related recombinant fragment protect human vascular smooth muscle cells from calcium-/phosphate-induced osteochondrocytic transdifferentiation.

    abstract::Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Freise C,Bobb V,Querfeld U

    更新日期:2017-09-15 00:00:00

  • A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the ran GTPase system.

    abstract::Ran, a nuclear GTPase, and a number of interacting proteins, including regulators RanGEF1 and RanGAP1, are involved in nucleocytoplasmic transport. We have identified a new temperature-sensitive mutation in budding yeast YRB1 gene, which encodes Ran-binding protein-1 (RanBP1). In contrast to other yrb1 alleles, the ne...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ouspenski II

    更新日期:1998-10-10 00:00:00