Abstract:
:A temperature-sensitive mutant, tsJT60, grew exponentially at both 34 degrees and 39.5 degrees C, but when stimulated from the resting state it entered S phase at 34 degrees but not at 39.5 degrees C. The mutated function appeared to be a prerequisite throughout from 0 to 9 h following the stimulation, in order that G0-arrested cells would enter S phase. When the arrested cells were stimulated with serum, the amount of and synthesis of protein increased at 34 degrees but not at 39.5 degrees C. The amount of polysome fraction was much smaller in stimulated and unstimulated cells at 39.5 degrees C than in those stimulated at 34 degrees C. Of the events reported to increase shortly after the stimulation, uridine transport increased at both temperatures. Mutation in tsJT60 cells may be concerned with the function prerequisite to induce protein synthesis following serum stimulation, resulting in the blocking of cell cycle progression toward S phase at 39.5 degrees C.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ninomiya-Tsuji J,Goto Y,Ishibashi S,Ide Tdoi
10.1016/0014-4827(86)90543-4subject
Has Abstractpub_date
1986-07-01 00:00:00pages
191-8issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(86)90543-4journal_volume
165pub_type
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