Autocrine regulation of the production of the gaseous messenger nitric oxide in a glioblastoma cell line.

abstract：:The tyrosine phosphatase PTP1B is an important regulator of cell function. In living cells PTP1B activity is restricted to the vicinity of the endoplasmic reticulum (ER) by post-translational C-terminal attachment of PTP1B to the ER membrane network. In our study we investigated the membrane anchor of PTP1B by use of ...

journal_title：Experimental cell research

authors： Anderie I,Schulz I,Schmid A

更新日期：2007-09-10 00:00:00

abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...

journal_title：Experimental cell research

authors： Sato C,Nishizawa K,Nakayama T,Hirai R,Nakamura H

更新日期：1986-12-01 00:00:00

abstract：:A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...

journal_title：Experimental cell research

authors： Nagasaki A,Uyeda TQ

更新日期：2008-03-10 00:00:00

abstract：:Gamma delta (γδ) T cell-based tumor immunotherapy has been one of the most promising cancer immunotherapeutic strategies. However, the key regulators of the Vγ9Vδ2 T cell-mediated antitumor response remain unclear. Recently, mounting reports have indicated that Tim-3 performs critical roles in the regulation of the ac...

journal_title：Experimental cell research

authors： Li X,Lu H,Gu Y,Zhang X,Zhang G,Shi T,Chen W

更新日期：2020-01-01 00:00:00

abstract：:Senescent human diploid fibroblasts, TIG-1, had labelling indices of about 0.5-3% when labelled with [3H]thymidine for 3 days in fresh medium containing 10% fetal bovine serum. When these cells were infected with SV40, the percentage of nuclei incorporating [3H]thymidine increased by about 10-fold. The frequency of T-...

journal_title：Experimental cell research

authors： Tsuji Y,Ide T,Ishibashi S

更新日期：1983-03-01 00:00:00

abstract：:Studies performed over the past five years have demonstrated that there are two major cell-intrinsic pathways for inducing apoptosis, one that begins with ligation of cell surface death receptors and another that involves mitochondrial release of cytochrome c. Several reports have suggested that anticancer drugs kill ...

journal_title：Experimental cell research

authors： Kaufmann SH,Earnshaw WC

更新日期：2000-04-10 00:00:00

abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...

journal_title：Experimental cell research

authors： Folkman J

更新日期：2006-03-10 00:00:00

abstract：:Our results showed that the expression of the src gene in chick embryo fibroblasts (CEF) released the density-dependent inhibition (DDI) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). With increasing cell density, phosphate metabolism decreased by 58% in normal CEF and, in c...

journal_title：Experimental cell research

authors： Jullien M,Harel L,Golde A,Villaudy J,Pugnet P

更新日期：1984-06-01 00:00:00

abstract：:This study investigates how epithelial cells moving together function to coordinate their collective movement to repair a wound. Using a lens ex vivo mock cataract surgery model we show that region-specific reorganization of cell-cell junctions, cytoskeletal networks and myosin function along apical and basal domains ...

journal_title：Experimental cell research

authors： Menko AS,Bleaken BM,Walker JL

更新日期：2014-03-10 00:00:00

abstract：:It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

journal_title：Experimental cell research

authors： Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

更新日期：1999-02-25 00:00:00

abstract：:Retromer is a multimeric protein complex that mediates intracellular receptor sorting. One of the roles of retromer is to promote transcytosis of the polymeric immunoglobulin receptor (pIgR) and its ligand polymeric immunoglobulin A (pIgA) in polarized epithelial cells. In Madin-Darby Canine Kidney (MDCK) cells, overe...

journal_title：Experimental cell research

authors： Vergés M,Sebastián I,Mostov KE

更新日期：2007-02-15 00:00:00

abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

journal_title：Experimental cell research

authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

更新日期：2018-03-01 00:00:00

abstract：:Autophagy is the principle pathway within cells involved in clearing damaged proteins and organelles. Therefore autophagy is necessary to maintain the turnover balance of peptides and homoeostasis. Autophagy occurs at basal levels under normal conditions but can be upregulated by chemical inducers or stress conditions...

journal_title：Experimental cell research

authors： Ganesan S,Moussavi Nik SH,Newman M,Lardelli M

更新日期：2014-10-15 00:00:00

abstract：:We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be...

journal_title：Experimental cell research

authors： Viallet J,Liu C,Emond J,Tsao MS

更新日期：1994-05-01 00:00:00

abstract：:Human calmodulin-like protein (CLP) is a calcium-binding protein down-regulated in a cell culture model of mammary tumorigenesis as well as in a majority of breast cancers in vivo. CLP down-regulation may be a result of the poorly differentiated state of these cell lines and tumors, or CLP expression may be incompatib...

journal_title：Experimental cell research

authors： Rogers MS,Kobayashi T,Pittelkow MR,Strehler EE

更新日期：2001-07-15 00:00:00

abstract：:The mitochondrion depends upon the import of cytosolically synthesized preproteins for most of the proteins that comprise its structural elements and metabolic pathways. Here we have examined the influence of redox conditions on mitochondrial preprotein import and processing by mammalian mitochondria. Paraquat pretrea...

journal_title：Experimental cell research

authors： Wright G,Terada K,Yano M,Sergeev I,Mori M

更新日期：2001-02-01 00:00:00

abstract：:Tubular epithelial-to-mesenchymal transition (EMT) plays a crucial role in the progression of renal tubular interstitial fibrosis (TIF), which subsequently leads to chronic kidney disease (CKD) and eventually, end-stage renal disease (ESRD). We propose that augmenter of liver regeneration (ALR), a member of the newly ...

journal_title：Experimental cell research

authors： Liao XH,Zhang L,Chen GT,Yan RY,Sun H,Guo H,Liu Q

更新日期：2014-10-01 00:00:00

abstract：:Actions of transforming growth factor-β are largely context dependent. For instance, TGF-β is growth inhibitory to epithelial cells and many tumor cell-lines while it stimulates the growth of mesenchymal cells. TGF-β also activates fibroblast cells to a myofibroblastic phenotype. In order to understand how the respons...

journal_title：Experimental cell research

authors： Chopra S,Kumar N,Rangarajan A,Kondaiah P

更新日期：2015-06-10 00:00:00

abstract：:It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...

journal_title：Experimental cell research

authors： Marini M,Frabetti F,Canaider S,Dini L,Falcieri E,Poirier GG

更新日期：2001-06-10 00:00:00

abstract：:Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage respon...

journal_title：Experimental cell research

authors： Beck C,Robert I,Reina-San-Martin B,Schreiber V,Dantzer F

更新日期：2014-11-15 00:00:00

abstract：:Epithelial ovarian cancer is the most common form of gynaecological malignancy. This lethal disease is thought to arise in ovarian surface epithelial (OSE) cells. The biology of these cells is not well understood, due to the limited amount of tissue that can be obtained from a single biopsy and their limited life span...

journal_title：Experimental cell research

authors： Davies BR,Steele IA,Edmondson RJ,Zwolinski SA,Saretzki G,von Zglinicki T,O'Hare MJ

更新日期：2003-08-15 00:00:00

abstract：:Relay of information from the extracellular environment into the cell often results from a peptide growth factor binding to its cognate cell surface receptor; this event is an integral mechanism by which many cellular functions occur, including cell growth, motility, and survival. In recent years, however, this requir...

journal_title：Experimental cell research

authors： Boerner JL,Danielsen A,Maihle NJ

更新日期：2003-03-10 00:00:00

abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

journal_title：Experimental cell research

authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

更新日期：2017-12-01 00:00:00

abstract：:A portion of the DNA within intact nuclei of a spontaneously transformed Chinese hamster ovary cell line (CHO-Kl) is relatively resistant to digestion by pancreatic deoxyribonuclease, as compared to nuclei from primary cultures of Chinese hamster ovary fibroblasts. Treatment of CHO-Kl cells with derivatives of 3',5' c...

journal_title：Experimental cell research

authors： Schonberg S,Patterson D,Puck TT

更新日期：1983-04-15 00:00:00

abstract：:Fish keratocytes are an established model in single cell motility but little is known about their collective migration. Initially, sheets migrate from the scale at ~145 μm/h but over the course of 24h the rate of leading edge advance decreases to ~23 μm/h. During this period, leader cells retain their ability to migra...

journal_title：Experimental cell research

authors： Rapanan JL,Cooper KE,Leyva KJ,Hull EE

更新日期：2014-08-01 00:00:00

abstract：:Fibronectin and alpha 1(I) and alpha 2(I) collagen proteins and RNAs are highly expressed during the growth phase of the non-transformed L6 alpha 1 rat myoblasts. When L6 alpha 1 cells from myotubes following transfer to low serum medium, the levels of fibronectin RNA decrease 8-fold, those of both alpha 2(I) transcri...

journal_title：Experimental cell research

authors： Leibovitch SA,Hillion J,Leibovitch MP,Guillier M,Schmitz A,Harel J

更新日期：1986-10-01 00:00:00

abstract：:The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...

journal_title：Experimental cell research

authors： Coppock DL,Buffolino P,Kopman C,Nathanson L

更新日期：1995-11-01 00:00:00

abstract：:Nod-like receptor pyrin domain-containing protein 6 (NLRP6) plays a key role in innate immunity, host defense and tumorigenesis. Our previous study has demonstrated the tumor suppressor role of NLRP6 in gastric cancer. In the present study, we explored the interaction protein of NLRP6 by Flag-tagged immunoprecipitatio...

journal_title：Experimental cell research

authors： Wang X,Wu X,Wang Q,Zhang Y,Wang C,Chen J

更新日期：2020-10-01 00:00:00

abstract：:In addition to previously isolated ratjadone A we describe three new members of this family, ratjadones B, C, and D, from another strain of the myxobacterium Sorangium cellulosum. We have investigated the properties of these ratjadones with respect to their activity on mammalian cell lines. We found IC(50) values in t...

journal_title：Experimental cell research

authors： Köster M,Lykke-Andersen S,Elnakady YA,Gerth K,Washausen P,Höfle G,Sasse F,Kjems J,Hauser H

更新日期：2003-06-10 00:00:00

abstract：:Olfactory ensheathing cells (OECs) are a type of glial cells with morphological plasticity in the olfactory system. Cultured OECs display the process-bearing and flattened shape. Our previous studies have shown that the frontal application of Slit-2 gradient induced the collapse of leading front, and reversed the soma...

journal_title：Experimental cell research

authors： Wang Y,Huang ZH

更新日期：2012-09-10 00:00:00