Autocrine regulation of the production of the gaseous messenger nitric oxide in a glioblastoma cell line.

Abstract:

:We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. the addition of the L-arginine analogs L-NG-monomethyl-L-arginine and L-N omega-nitro-L-arginine and dexamethasone to the GBM cultures caused a substantial inhibition of NO production. The addition of monoclonal antibodies against IL-1 and TNF alpha to the cultures resulted in an inhibition of NO production, whereas the addition of anti-TGF beta monoclonal antibodies resulted in an increase in NO production. These findings suggest the presence of an autocrine regulatory mechanism for NO production in some tumor cell lines.

journal_name

Exp Cell Res

authors

Haregewoin A,Alexander E 3rd,Black PM,Loeffler JS

doi

10.1006/excr.1994.1020

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

137-9

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(84)71020-2

journal_volume

210

pub_type

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