Autocrine regulation of the production of the gaseous messenger nitric oxide in a glioblastoma cell line.


:We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. the addition of the L-arginine analogs L-NG-monomethyl-L-arginine and L-N omega-nitro-L-arginine and dexamethasone to the GBM cultures caused a substantial inhibition of NO production. The addition of monoclonal antibodies against IL-1 and TNF alpha to the cultures resulted in an inhibition of NO production, whereas the addition of anti-TGF beta monoclonal antibodies resulted in an increase in NO production. These findings suggest the presence of an autocrine regulatory mechanism for NO production in some tumor cell lines.


Exp Cell Res


Haregewoin A,Alexander E 3rd,Black PM,Loeffler JS




Has Abstract


1994-01-01 00:00:00














  • Human immunodeficiency virus type 1 Tat protein modulates cell cycle and apoptosis in Epstein-Barr virus-immortalized B cells.

    abstract::Patients infected with human immunodeficiency virus type 1 (HIV-1) develop a spectrum of B cell lymphoproliferative disorders ranging from polyclonal B cell activation to B cell lymphomas. While a direct role of Epstein-Barr virus (EBV) is well recognized for most of these lesions, recent findings have suggested that ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Colombrino E,Rossi E,Ballon G,Terrin L,Indraccolo S,Chieco-Bianchi L,De Rossi A

    更新日期:2004-05-01 00:00:00

  • A role for both RB and p53 in the regulation of human cellular senescence.

    abstract::We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore prol...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shay JW,Pereira-Smith OM,Wright WE

    更新日期:1991-09-01 00:00:00

  • Down-regulation of UDP-glucose dehydrogenase affects glycosaminoglycans synthesis and motility in HCT-8 colorectal carcinoma cells.

    abstract::UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang TP,Pan YR,Fu CY,Chang HY

    更新日期:2010-10-15 00:00:00

  • Signaling interplay between PARP1 and ROS regulates stress-induced cell death and developmental changes in Dictyostelium discoideum.

    abstract::Poly (ADP-ribose) polymerase-1 (PARP1) is a DNA damage sensor that gets activated in proportion to the damage, helping cells to determine whether to repair the damage or initiate cell death processes. We have previously shown PARP1's significance in the developmental processes of Dictyostelium discoideum in addition t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mir H,Rajawat J,Vohra I,Vaishnav J,Kadam A,Begum R

    更新日期:2020-12-15 00:00:00

  • Effect of tunicamycin on cell cycle progression in budding yeast.

    abstract::Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vai M,Popolo L,Alberghina L

    更新日期:1987-08-01 00:00:00

  • Interferon alpha induces cell death through interference with interleukin 6 signaling and inhibition of STAT3 activity.

    abstract::In multiple myeloma, which commonly depends on interleukin 6, IL-6, survival signaling induced by this cytokine is largely mediated by activation of STAT3. Interferon alpha (IFNalpha) treatment of cell lines derived from multiple myeloma or of myeloma tumor cells ex vivo leads to apoptosis. In this study we demonstrat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thyrell L,Arulampalam V,Hjortsberg L,Farnebo M,Grandér D,Pokrovskaja Tamm K

    更新日期:2007-11-15 00:00:00

  • NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation.

    abstract:BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

    更新日期:2019-10-01 00:00:00

  • GADD34 mediates cytoprotective autophagy in mutant huntingtin expressing cells via the mTOR pathway.

    abstract::Increased protein aggregation and altered cell signaling accompany many neurodegenerative diseases including Huntington's disease (HD). Cell stress is counterbalanced by signals mediating cell repair but the identity of these are not fully understood. We show here that the mammalian target of rapamycin (mTOR) pathway ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hyrskyluoto A,Reijonen S,Kivinen J,Lindholm D,Korhonen L

    更新日期:2012-01-01 00:00:00

  • The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor.

    abstract::Activation of growth factor receptors, such as platelet-derived growth factor (PDGF) receptors, has a major impact on the motile behavior of vertebrate cells. The WASP family of proteins has been recognized as important regulators of actin polymerization via the activation of the Arp2/3 complex. The activity of the WA...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Aspenström P

    更新日期:2002-09-10 00:00:00

  • Basic FGF and TGF-beta differentially modulate integrin expression of human microvascular endothelial cells.

    abstract::Basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) are known to alter the migratory and proliferative capacity of endothelial cells in vitro and to stimulate angiogenesis in vivo. One mechanism by which these cytokines induce their effects may be through the regulation of integrin adh...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Enenstein J,Waleh NS,Kramer RH

    更新日期:1992-12-01 00:00:00

  • Mcl-1 antagonism is a potential therapeutic strategy in a subset of solid cancers.

    abstract::Cancer cell survival is frequently dependent on the elevated levels of members of the Bcl-2 family of prosurvival proteins that bind to and inactivate BH3-domain pro-apoptotic cellular proteins. Small molecules that inhibit the protein-protein interactions between prosurvival and proapoptotic Bcl-2 family members (so-...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Modugno M,Banfi P,Gasparri F,Borzilleri R,Carter P,Cornelius L,Gottardis M,Lee V,Mapelli C,Naglich JG,Tebben A,Vite G,Pastori W,Albanese C,Corti E,Ballinari D,Galvani A

    更新日期:2015-03-15 00:00:00

  • Induction of apoptosis in human replicative senescent fibroblasts.

    abstract::Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

    更新日期:2002-03-10 00:00:00

  • Phenotype-dependent expression of alpha-smooth muscle actin in visceral smooth muscle cells.

    abstract::Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue K

    更新日期:1999-02-25 00:00:00

  • Role of esterase gp70 and its influence on growth and development of Dictyostelium discoideum.

    abstract::Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of v...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yuan A,Chia CP

    更新日期:2000-12-15 00:00:00

  • Constitutively active BMP type I receptors transduce BMP-2 signals without the ligand in C2C12 myoblasts.

    abstract::Bone morphogenetic protein-2 (BMP-2), a member of transforming growth factor-beta superfamily, inhibits the terminal differentiation of C2C12 myoblasts and changes their differentiation pathway into cells expressing osteoblast phenotypes such as alkaline phosphatase (ALP) activity and osteocalcin production (Katagiri ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Akiyama S,Katagiri T,Namiki M,Yamaji N,Yamamoto N,Miyama K,Shibuya H,Ueno N,Wozney JM,Suda T

    更新日期:1997-09-15 00:00:00

  • Recent progress in protocadherin research.

    abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Suzuki ST

    更新日期:2000-11-25 00:00:00

  • Prosaposin: a new player in cell death prevention of U937 monocytic cells.

    abstract::We report that prosaposin binds to U937 and is active as a protective factor on tumor necrosis factor alpha (TNFalpha)-induced cell death. The prosaposin-derived saposin C binds to U937 cells in a concentration-dependent manner, suggesting that prosaposin behaves similarly. Prosaposin binding induces U937 cell death p...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

    更新日期:2004-08-01 00:00:00

  • The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis.

    abstract::The coxsackie- and adenovirus receptor (CAR) is a transmembrane protein belonging to the immunoglobulin superfamily. The function of CAR as a virus receptor has been extensively analyzed, while its physiological role and expression pattern in adult tissues have remained less clear. CAR associates with epithelial tight...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Raschperger E,Thyberg J,Pettersson S,Philipson L,Fuxe J,Pettersson RF

    更新日期:2006-05-15 00:00:00

  • Ultrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.

    abstract::The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleola...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Vandelaer M,Thiry M,Goessens G

    更新日期:1993-04-01 00:00:00

  • Nuclear domains in skeletal myotubes: the localization of transferrin receptor mRNA is independent of its half-life and restricted by binding to ribosomes.

    abstract::The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ralston E,McLaren RS,Horowitz JA

    更新日期:1997-11-01 00:00:00

  • Metabolic compartmentation in living cells: structural association of aldolase.

    abstract::The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang J,Tolan DR,Pagliaro L

    更新日期:1997-12-15 00:00:00

  • The nuclear factor kappa B (NF-κB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells.

    abstract::Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor which controls the expression of various genes involved in immune responses. However, it is not clear whether NF-κB activation is critical for phagocytosis when Staphylococcus aureus is the pathogen. Using oligonucleotide microarrays, we investigated ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhu F,Yue W,Wang Y

    更新日期:2014-10-01 00:00:00

  • Effects of succinylacetone on dimethylsulfoxide-mediated induction of heme pathway enzymes in mouse friend virus-transformed erythroleukemia cells.

    abstract::Heme has been reported to exert a control over its own biosynthesis and to affect the erythroid differentiation process at different sites. In this study, succinylacetone, a powerful inhibitor of delta-aminolevulinic acid dehydrase was used to block heme synthesis and to study the effects of heme depletion on the dime...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Beaumont C,Deybach JC,Grandchamp B,da Silva V,de Verneuil H,Nordmann Y

    更新日期:1984-10-01 00:00:00

  • MUC6 mucin expression inhibits tumor cell invasion.

    abstract::The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Leir SH,Harris A

    更新日期:2011-10-15 00:00:00

  • BST-2/Tetherin is involved in BAFF-enhanced proliferation and survival via canonical NF-κB signaling in neoplastic B-lymphoid cells.

    abstract::The development of Sjögren's syndrome (SS) is accompanied by B cell hyperproliferation and mutation. Our previous study identified aberrant expression of BST-2 (also known as Tetherin/CD317) in B cells from either the peripheral blood or infiltrated salivary glands. However, the roles of BST-2 in the regulation of B c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fu J,Shi H,Zhan T,Li H,Ye L,Xie L,Wang Z,Wang B,Zheng L

    更新日期:2021-01-01 00:00:00

  • Nanosecond pulsed electric field (nsPEF) enhance cytotoxicity of cisplatin to hepatocellular cells by microdomain disruption on plasma membrane.

    abstract::Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma me...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yin S,Chen X,Xie H,Zhou L,Guo D,Xu Y,Wu L,Zheng S

    更新日期:2016-08-15 00:00:00

  • Dissecting the sequence specific functions of alternative N-terminal isoforms of mouse bullous pemphigoid antigen 1.

    abstract::Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins that is involved in cross-linking the cytoskeletal elements and attaching them to cell junctions. BPAG1 null mice develop severe degeneration of sensory neurons that was attributed in part due to the absence of a splice variant called BP...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jefferson JJ,Leung CL,Liem RK

    更新日期:2006-09-10 00:00:00

  • Secretory organelle docking at the cell membrane of Paramecium cells: dedocking and synchronized redocking of trichocysts.

    abstract::We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pape R,Plattner H

    更新日期:1990-12-01 00:00:00

  • Overexpression of soluble ADAM33 promotes a hypercontractile phenotype of the airway smooth muscle cell in rat.

    abstract::A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Duan Y,Long J,Chen J,Jiang X,Zhu J,Jin Y,Lin F,Zhong J,Xu R,Mao L,Deng L

    更新日期:2016-11-15 00:00:00

  • DEPDC1B knockdown inhibits the development of malignant melanoma through suppressing cell proliferation and inducing cell apoptosis.

    abstract::Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xu Y,Sun W,Zheng B,Liu X,Luo Z,Kong Y,Xu M,Chen Y

    更新日期:2019-06-01 00:00:00