Abstract:
:We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. the addition of the L-arginine analogs L-NG-monomethyl-L-arginine and L-N omega-nitro-L-arginine and dexamethasone to the GBM cultures caused a substantial inhibition of NO production. The addition of monoclonal antibodies against IL-1 and TNF alpha to the cultures resulted in an inhibition of NO production, whereas the addition of anti-TGF beta monoclonal antibodies resulted in an increase in NO production. These findings suggest the presence of an autocrine regulatory mechanism for NO production in some tumor cell lines.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Haregewoin A,Alexander E 3rd,Black PM,Loeffler JSdoi
10.1006/excr.1994.1020subject
Has Abstractpub_date
1994-01-01 00:00:00pages
137-9issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(84)71020-2journal_volume
210pub_type
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