Characterization of the intermediate filament proteins of murine mammary gland epithelial cells. Response to collagen substratum.

abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

journal_title：Experimental cell research

authors： Udy GB,Parkes BD,Wells DN

更新日期：1997-03-15 00:00:00

abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

journal_title：Experimental cell research

authors： Zhang J,Harrison JS,Studzinski GP

更新日期：2011-01-01 00:00:00

abstract：:Nuclear pore complexes (NPCs) provide the only sites for macromolecular transport between nucleus and cytoplasm. The nucleoporin p62, a component of higher eukaryotic NPCs, is located at the central gated channel and involved in nuclear trafficking of various cargos. p62 is organized into an N-terminal segment that co...

journal_title：Experimental cell research

authors： Stochaj U,Bański P,Kodiha M,Matusiewicz N

更新日期：2006-08-01 00:00:00

abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

journal_title：Experimental cell research

authors： Tchang F,Méchali M

更新日期：1999-08-25 00:00:00

abstract：:Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...

journal_title：Experimental cell research

authors： Domazetovic V,Bonanomi AG,Stio M,Vincenzini MT,Iantomasi T

更新日期：2019-09-15 00:00:00

abstract：BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

journal_title：Experimental cell research

authors： Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

更新日期：2019-10-01 00:00:00

abstract：:A novel protein that associates with interphase nucleus and mitotic apparatus (INMAP) was identified by screening HeLa cDNA expression library with an autoimmune serum followed by tandem mass spectrometry. Its complete cDNA sequence of 1.818 kb encodes 343 amino acids with predicted molecular mass of 38.2 kDa and nume...

journal_title：Experimental cell research

authors： Shen E,Lei Y,Liu Q,Zheng Y,Song C,Marc J,Wang Y,Sun L,Liang Q

更新日期：2009-04-15 00:00:00

abstract：:The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. Polysomal mRNPs were predominantly associated wit...

journal_title：Experimental cell research

authors： Bagchi T,Larson DE,Sells BH

更新日期：1987-01-01 00:00:00

abstract：:p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...

journal_title：Experimental cell research

authors： Xia X,Carnahan RH,Vaughan MH,Wildenberg GA,Reynolds AB

更新日期：2006-10-15 00:00:00

abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

journal_title：Experimental cell research

authors： Qian J,Tu R,Yuan L,Xie W

更新日期：2016-06-10 00:00:00

abstract：:Parietal yolk sac cells M1536 -B3 grown on cytodex 2 beads deposited an extracellular matrix on the surface of the beads. Cell-free matrix-coated beads were isolated by treatment of the cell monolayer with cytochalasin B (CB) at a concentration of 10 micrograms/ml of phosphate-buffered saline (PBS). The matrix when an...

journal_title：Experimental cell research

authors： Mai S,Chung AE

更新日期：1984-06-01 00:00:00

abstract：:In a previous study, we found that a specific inhibitor of cellular arginine-specific mono(ADP-ribosyl) transferase, meta-iodobenzylguanidine (MIBG), reversibly inhibited both proliferation and differentiation of cultured embryonic chick primary muscle myoblasts. In addition, we observed that arginine-specific ADP-rib...

journal_title：Experimental cell research

authors： Huang HY,Zhou H,Huiatt TW,Graves DJ

更新日期：1996-07-10 00:00:00

abstract：:In contrast to extensive studies on the roles of molecular chaperones, such as heat shock proteins, there are only a few reports about the roles of GRP78/BiP, an endoplasmic reticulum (ER) stress-induced molecular chaperone, in mammalian cell responses to DNA-damaging stresses. To investigate whether GRP78/BiP is invo...

journal_title：Experimental cell research

authors： Zhai L,Kita K,Wano C,Wu Y,Sugaya S,Suzuki N

更新日期：2005-05-01 00:00:00

abstract：:Compact mouse morulae were decompacted in calcium-free medium and allowed to recompact in standard embryo culture medium. When the recompaction medium contained trifluoperazine (TFP)(0.05 mM), an inhibitor of the calcium-dependent protein calmodulin, the embryos failed to recompact. This effect could not be overcome b...

journal_title：Experimental cell research

authors： Bilozur M,Powers RD

更新日期：1982-11-01 00:00:00

abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

journal_title：Experimental cell research

authors： Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

更新日期：2004-07-15 00:00:00

abstract：:Interferon beta is widely used as first-line treatment for relapsing remitting multiple sclerosis (RRMS). Several products are marketed world-wide, and biosimilar products are emerging. Interferon beta reduces relapse rates by about 1/3, and reduces the appearance of new MRI lesions by about 2/3, and some studies have...

journal_title：Experimental cell research

authors： Rudick RA,Goelz SE

更新日期：2011-05-15 00:00:00

abstract：:The formation of capillaries during development and tissue repair is likely to involve active reorganization of the actin cytoskeleton, although few studies have addressed this issue. Here, we have utilized an in vitro model of capillary morphogenesis whereby human umbilical vein endothelial cells are suspended within...

journal_title：Experimental cell research

authors： Salazar R,Bell SE,Davis GE

更新日期：1999-05-25 00:00:00

abstract：:Hepatocyte growth factor (HGF) is a pleiotropic cytokine promoting proliferation, migration and survival in several cell types. HGF and its cognate receptor c-Met are expressed in cardiac cells during early cardiogenesis, but data concerning its role in cardiac differentiation of embryonic stem cells (ESCs) and the un...

journal_title：Experimental cell research

authors： Roggia C,Ukena C,Böhm M,Kilter H

更新日期：2007-03-10 00:00:00

abstract：:We have partially purified a specific cyclin B2 kinase (cyk) from prophase oocytes of Xenopus laevis after an ATP-gamma-S activation step. Phosphopeptide analysis identified Ser53 as the major in vitro phosphorylation site for cyk in cyclin B2. Using a synthetic peptide derived from cyclin B2 encompassing Ser53 (cykti...

journal_title：Experimental cell research

authors： Derua R,Stevens I,Waelkens E,Fernandez A,Lamb N,Merlevede W,Goris J

更新日期：1997-02-01 00:00:00

abstract：:Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population and show significant expansion under pathological conditions. microRNA plays important roles in many biological processes, whether microRNAs have a function in the expansion of MDSCs is still not very clear. In this study, miR-34a overexpression c...

journal_title：Experimental cell research

authors： Huang A,Zhang H,Chen S,Xia F,Yang Y,Dong F,Sun D,Xiong S,Zhang J

更新日期：2014-08-15 00:00:00

abstract：:Transferrin (Tf), an iron-binding protein, was investigated in the cultured human fibroblast which is a major cell type in connective tissues. Tf is a major iron-transporting protein and has an important role in iron metabolism. Using two-dimensional gel electrophoresis, we demonstrated the eight subtypes of Tf produc...

journal_title：Experimental cell research

authors： Kondo T,Sakaguchi M,Namba M

更新日期：1998-07-10 00:00:00

abstract：:Previous studies have established that Schwann cells (SC) in culture express an NGF receptor. In this study, cultures of fetal human SC were established from fetal nerves and various light microscopic (LM) and electron microscopic (EM) techniques were used to localize the NGF receptor on the SC. Results indicate that ...

journal_title：Experimental cell research

authors： Assouline JG,Pantazis NJ

更新日期：1989-06-01 00:00:00

abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...

journal_title：Experimental cell research

authors： Song Z,Cao Q,Ruan H,Yang H,Wang K,Bao L,Cheng G,Xu T,Xiao H,Wang C,Liu D,Chen K,Zhang X

更新日期：2018-11-15 00:00:00

abstract：:Celiac disease (CD) is a multisystemic autoimmune inflammation of the intestinal tract induced by wheat gluten and related cereals in HLA-DQ2/8 positive individuals. An essential role in the pathogenesis of CD is played by a fraction of the peptic-tryptic digest of gluten, Frazer's Fraction (FF). Here, we investigate ...

journal_title：Experimental cell research

authors： Reinke Y,Zimmer KP,Naim HY

更新日期：2009-11-15 00:00:00

abstract：:Nucleotide sugar transporters play a central role in the process of glycosylation. They are responsible for the translocation of nucleotide sugars from the cytosol, their site of synthesis, into the Golgi apparatus where the activated sugars serve as substrates for a variety of glycosyltransferases. We and others have...

journal_title：Experimental cell research

authors： Lühn K,Laskowska A,Pielage J,Klämbt C,Ipe U,Vestweber D,Wild MK

更新日期：2004-12-10 00:00:00

abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

journal_title：Experimental cell research

authors： Saggioro D,D'agostino DM,Chieco-Bianchi L

更新日期：1999-03-15 00:00:00

abstract：:The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...

journal_title：Experimental cell research

authors： Zhao C,Hashiguchi A,Kondoh K,Du W,Hata J,Yamada T

更新日期：2002-05-01 00:00:00

abstract：:Pirh2, a recently identified ubiquitin-protein ligase, has been reported to promote p53 degradation. Pirh2 physically interacts with p53 and promotes ubiquitination of p53 independently of MDM2. Like MDM2, Pirh2 is thought to participate in an autoregulatory feedback loop that controls p53 function. We have previously...

journal_title：Experimental cell research

authors： Duan W,Gao L,Wu X,Zhang Y,Otterson GA,Villalona-Calero MA

更新日期：2006-10-15 00:00:00

abstract：:We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...

journal_title：Experimental cell research

authors： You N,Tan Y,Zhou L,Huang X,Wang W,Wang L,Wu K,Mi N,Li J,Zheng L

更新日期：2017-09-15 00:00:00

abstract：OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

journal_title：Experimental cell research

authors： Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

更新日期：2012-02-15 00:00:00