当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Identification and molecular cloning of a functional GDP-fucose transporter in Drosophila melanogaster.

Identification and molecular cloning of a functional GDP-fucose transporter in Drosophila melanogaster.

Abstract：

:Nucleotide sugar transporters play a central role in the process of glycosylation. They are responsible for the translocation of nucleotide sugars from the cytosol, their site of synthesis, into the Golgi apparatus where the activated sugars serve as substrates for a variety of glycosyltransferases. We and others have recently identified and cloned the first GDP-fucose transporters of H. sapiens and C. elegans. Based on sequence similarity, we could identify a putative homolog in Drosophila melanogaster showing about 45% identity on protein level. The gene (CG9620) encodes a highly hydrophobic, multi-transmembrane spanning protein of 38.1 kDa that is localized in the Golgi apparatus. In order to test whether this protein serves as a GDP-fucose transporter, we performed complementation studies with fibroblasts from a patient with LADII (leukocyte adhesion deficiency II) which exhibit a strong reduction of fucosylation due to a point mutation in the human GDP-fucose transporter gene. We show that transient transfection of these cells with the Drosophila CG9620 cDNA corrects the GDP-fucose transport defect and reestablishes fucosylation. This study gives experimental proof that the product of the in silico identified Drosophila gene CG9620 serves as a functional GDP-fucose transporter.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Lühn K,Laskowska A,Pielage J,Klämbt C,Ipe U,Vestweber D,Wild MK

doi

10.1016/j.yexcr.2004.08.043

keywords:

subject

Has Abstract

pub_date

2004-12-10 00:00:00

pages

242-50

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(04)00445-8

journal_volume

301

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Smads oppose Hox transcriptional activities.

    abstract：:BMPs and Hox proteins play crucial roles in developmental processes. Beyond their mutual regulation of gene expression, little is known about the relations between their mechanisms of actions. Previously, we have shown that Hoxc8 acts as a downstream repressor in the BMP signaling pathway. Smad1 and Smad6 interact wit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.12.002

    authors： Li X,Nie S,Chang C,Qiu T,Cao X

    更新日期：2006-04-01 00:00:00

  • PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes.

    abstract：:Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5570

    authors： Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

    更新日期：2002-08-15 00:00:00

  • Adenovirus-mediated transfer of Bcl-X(L) protects neuronal cells from Bax-induced apoptosis.

    abstract：:Bax-mediated apoptosis in neurons is involved in many pathologic conditions affecting the central nervous system, including degenerative diseases, stroke, and trauma. Two molecules belonging to the Bcl-2 family, Bcl-2 and Bcl-X(L), protect cells from Bax-induced apoptosis and show distinct expression patterns in adult...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4751

    authors： Shinoura N,Satou R,Yoshida Y,Asai A,Kirino T,Hamada H

    更新日期：2000-02-01 00:00:00

  • MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages.

    abstract：:Sepsis is a major cause of mortality in seriously ill patients characterized by a series of severe systemic inflammatory responses due to an infection. Thus, there is a critically need to search more accurate biomarkers and targets for diagnosis and treatment of sepsis. Our study showed that miR-212-3p was up-regulate...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.12.008

    authors： Chen W,Ma X,Zhang P,Li Q,Liang X,Liu J

    更新日期：2017-01-15 00:00:00

  • Analysis of actin microfilaments and cell-to-substrate adhesive structures in human fibroblasts from individuals genetically predisposed to colonic carcinoma.

    abstract：:Skin fibroblasts from patients with inherited adenomatosis of the large bowel (ACR-SF) possess alterations in actin microfilament (MF) organization which serve to distinguish "predisposed" cells from fibroblasts derived from normal individuals (NSF). MF bundle frequency and diameter were considerably reduced in ACR-SF...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90389-c

    authors： Higgins PJ,Kopelovich L

    更新日期：1991-08-01 00:00:00

  • Characterization of apoptotic pathway associated with caspase-independent excision of DNA loop domains.

    abstract：:Excision of chromatin loop domains and internucleosomal DNA fragmentation are widely considered as consecutive stages of chromatin disassembly during apoptosis. We report here on apoptosis induced by staurosporine in NB-2a neuroblastoma cells, which was accompanied by excision of chromatin loop domains, but proceeded ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.01.025

    authors： Solovyan VT

    更新日期：2007-04-15 00:00:00

  • Intracellular insulin quantification by cell-ELISA.

    abstract：:Current methods of monitoring insulin in culture are limited to soluble insulin (secretions or lysates) or synthetic gene reporter analyses. We present an insulin-specific cell enzyme-linked immunosorbent assay (cell-ELISA) to assess relative intracellular insulin protein content of adherent cultures, normalized to ce...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.06.014

    authors： Andersen PL,Vermette P

    更新日期：2016-09-10 00:00:00

  • Furin inhibition prevents hypoxic and TGFβ-mediated blood-brain barrier disruption.

    abstract：:Hypoxic blood-brain barrier (BBB) dysfunction is a common feature of CNS diseases however mechanisms underlying barrier disturbance are still largely unknown. This study investigated the role of transforming growth factor β (TGFβ), a cytokine known to induce expression of the proprotein convertase Furin, in hypoxia-me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111503

    authors： Baumann J,Huang SF,Gassmann M,Tsao CC,Ogunshola OO

    更新日期：2019-10-15 00:00:00

  • Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy.

    abstract：:The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90034-6

    authors： Kitajima Y,Owaribe K,Nishizawa Y,Jokura Y,Yaoita H

    更新日期：1992-11-01 00:00:00

  • Overexpression of miR-182 inhibits ossification of ligamentum flavum cells by targeting NAMPT.

    abstract：:Ossification of the ligamentum flavum (OLF) is a debilitating disease resulting from the development of ectopic bone formation, which leads to the compression of the spinal cord. Nicotinamide phosphoribosyltransferase (NAMPT) was found to be upregulated and microRNA-182 (miR-182) was downregulated in OLF tissue. We in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.03.008

    authors： Zhang Q,Shen Y,Jiang Y,Zhao S,Zhou D,Xu N

    更新日期：2018-06-15 00:00:00

  • A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes.

    abstract：:It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.04.002

    authors： Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

    更新日期：2006-08-01 00:00:00

  • Homocysteine regulates expression of Herp by DNA methylation involving the AARE and CREB binding sites.

    abstract：:Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.09.004

    authors： Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

    更新日期：2006-12-10 00:00:00

  • Cardiac apoptosis caused by elevated cholesterol level in experimental autoimmune myocarditis.

    abstract：:It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112169

    authors： Chang H,Wang Y,Wu Y,Ma P,Song Y,Liu C,Ye Y,Qi JH,Qi Z

    更新日期：2020-10-01 00:00:00

  • Serum effects on the in vitro differentiation of sea urchin micromeres.

    abstract：:When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90355-5

    authors： McCarthy RA,Spiegel M

    更新日期：1983-12-01 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract：:NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90140-5

    authors： Durkin JP,Whitfield JF

    更新日期：1985-04-01 00:00:00

  • Cell condensation in chondrogenic differentiation.

    abstract：:Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(05)80067-9

    authors： Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

    更新日期：1992-05-01 00:00:00

  • Research progress on the effect of autophagy-lysosomal pathway on tumor drug resistance.

    abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2020.111925

    authors： Li J,Liu G,Li L,Yao Z,Huang J

    更新日期：2020-04-15 00:00:00

  • Cell-scaffold interaction within engineered tissue.

    abstract：:The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.02.028

    authors： Chen H,Liu Y,Jiang Z,Chen W,Yu Y,Hu Q

    更新日期：2014-05-01 00:00:00

  • Nuclear topology of murine, cerebellar Purkinje neurons: changes as a function of development.

    abstract：:The interphase nucleus is a structurally ordered, three-dimensional structure, in which specific chromatin domains occupy distinct spatial positions that can, in turn, be modified with changes in cell function. A fundamental goal in developmental neurobiology is the identification of mechanisms that dictate the orderl...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4793

    authors： Martou G,De Boni U

    更新日期：2000-04-10 00:00:00

  • Permeable FGF-1 nuclear localization signal peptide stimulates DNA synthesis in various cell types but is cell-density sensitive and unable to support cell proliferation.

    abstract：:An earlier report indicated that a 26-amino-acid peptide (SA), comprised of the nuclear localization signal (NLS) of fibroblast growth factor-1 (FGF-1) and a membrane-permeable peptide, was able to stimulate DNA synthesis after it was taken up by NIH3T3 fibroblasts. Here, we report that SA, but not a mutant with the N...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4176

    authors： Komi A,Suzuki M,Imamura T

    更新日期：1998-09-15 00:00:00

  • The cytochrome b5 tail anchors and stabilizes subdomains of human DNA topoisomerase II alpha in the cytoplasm of retrovirally infected mammalian cells.

    abstract：:DNA topoisomerase II (topo II) is the target of many anticancer drugs and is often altered in drug-resistant cell lines. In some tumor cell lines truncated isoforms of topo IIalpha are localized to the cytoplasm. To study the localization and function of individual enzyme domains, we have epitope-tagged several fragme...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4446

    authors： Soltermann A,Ernst A,Leroy D,Stahel RA,Gasser SM

    更新日期：1999-06-15 00:00:00

  • Absence of the Shb gene in mixed-lineage leukemia MLL-AF9 cells increases latency in mice despite higher proliferation rates in vitro.

    abstract：:Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112368

    authors： Jamalpour M,Bergquist E,Welsh M

    更新日期：2020-12-15 00:00:00

  • Oxidative stress inhibits the mitochondrial import of preproteins and leads to their degradation.

    abstract：:The mitochondrion depends upon the import of cytosolically synthesized preproteins for most of the proteins that comprise its structural elements and metabolic pathways. Here we have examined the influence of redox conditions on mitochondrial preprotein import and processing by mammalian mitochondria. Paraquat pretrea...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5096

    authors： Wright G,Terada K,Yano M,Sergeev I,Mori M

    更新日期：2001-02-01 00:00:00

  • Microfilament regulatory protein MENA increases activity of RhoA and promotes metastasis of hepatocellular carcinoma.

    abstract：:Mammalian enabled (MENA), usually known as a direct regulator of microfilament polymerization and bundling, promotes metastasis in various cancers. Here we focus on the role of MENA in hepatocellular carcinoma (HCC) metastasis and the relevant mechanism from the view of RhoA activity regulation. By HCC tissue microarr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.05.010

    authors： Lin L,Yang XM,Li J,Zhang YL,Qin W,Zhang ZG

    更新日期：2014-09-10 00:00:00

  • HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for embryogenesis and oogenesis in Caenorhabditis elegans.

    abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.04.043

    authors： Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

    更新日期：2004-08-15 00:00:00

  • Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.

    abstract：:Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.020

    authors： Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

    更新日期：2010-07-15 00:00:00

  • Unidirectional co-stimulation by a non-mating strain of Tetrahymena thermophila.

    abstract：:We report that a fatty acid auxotroph of Tetrahymena thermophila (RH179E1) fails to mate, yet retains the ability to co-stimulate normal cells unidirectionally. Thus, co-stimulation can be analyzed experimentally in the absence of pair formation. We show that the co-stimulation of normal cells of one mating type is su...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90225-4

    authors： Katz M,Conner RL,Baum MP,Orias E

    更新日期：1987-03-01 00:00:00

  • CIN85 associates with TNF receptor 1 via Src and modulates TNF-alpha-induced apoptosis.

    abstract：:CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains. Here we report that it is also a component of the signaling complex associated with tumor necrosis factor receptor 1 (TNFR1), which lacks a tyrosine kinase domain. This was established by showing that CIN85 was co-precipita...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.11.005

    authors： Narita T,Nishimura T,Yoshizaki K,Taniyama T

    更新日期：2005-03-10 00:00:00

  • The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis.

    abstract：:The coxsackie- and adenovirus receptor (CAR) is a transmembrane protein belonging to the immunoglobulin superfamily. The function of CAR as a virus receptor has been extensively analyzed, while its physiological role and expression pattern in adult tissues have remained less clear. CAR associates with epithelial tight...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.01.025

    authors： Raschperger E,Thyberg J,Pettersson S,Philipson L,Fuxe J,Pettersson RF

    更新日期：2006-05-15 00:00:00

  • Nuclear import of p53 during Xenopus laevis early development in relation to DNA replication and DNA repair.

    abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4570

    authors： Tchang F,Méchali M

    更新日期：1999-08-25 00:00:00