Abstract：
:Mammalian enabled (MENA), usually known as a direct regulator of microfilament polymerization and bundling, promotes metastasis in various cancers. Here we focus on the role of MENA in hepatocellular carcinoma (HCC) metastasis and the relevant mechanism from the view of RhoA activity regulation. By HCC tissue microarray analysis, we found that MENA expression was positively associated with satellite lesions (P<0.01) and vascular invasion (P<0.01). Cases with membrane reinforcement of MENA staining in HCC tissues had significantly higher rates of early recurrence in the intermediate MENA expression group. Knockdown of MENA significantly suppressed HCC cell migration and invasion in vitro, as well as their intrahepatic and distant metastasis in vivo. Knockdown of MENA also decreased filopodia and stress fibers in SMMC-7721 cells. Furthermore, a decrease of RhoA activity was detected by a pull-down assay in SMMC-7721-shMENA cells. The ROCK inhibitor, Y-27632, suppressed migration of both MENA knockdown SMMC-7721 cells and control cells, but diminished their difference. Thus, our findings suggest that MENA promotes HCC cell motility by activating RhoA.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Lin L,Yang XM,Li J,Zhang YL,Qin W,Zhang ZGdoi
10.1016/j.yexcr.2014.05.010subject
Has Abstractpub_date
2014-09-10 00:00:00pages
113-22issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(14)00201-8journal_volume
327pub_type
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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