Serum effects on the in vitro differentiation of sea urchin micromeres.


:When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after isolation or by pulsing the cells with serum at specific times during their in vitro development. The optimum time of serum addition for spicule formation is 36 h after fertilization (AF). Further delay in the addition of serum results in a reduction in the number of spicules formed in culture and a delay in the time at which they appear. A 1-h pulse of serum at 36 h AF is sufficient to initiate a response in some of the micromere aggregates. A 12-h pulse at 36 h AF produces the maximum number of spicules per culture. The critical period for serum addition, 36-48 h AF, corresponds to the time in the normal embryo at which the syncytial primary mesenchyme ring is formed. Electron micrographs of cultured cells demonstrate that micromeres cultured without serum until 48 h AF fail to form pseudopodial extensions and remain as rosette-like clusters of cells. If serum is present, extensive pseudopodial networks form which resemble the primary ring syncytium. These results suggest that serum acts to stimulate fused pseudopodial networks in cultures of micromeres and that the resulting syncytium is necessary for spicule formation.


Exp Cell Res


McCarthy RA,Spiegel M




Has Abstract


1983-12-01 00:00:00














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    abstract::The phosphorylation state of the CD18-chain of beta 2-integrins have been shown not to mediate changes in the avidity of these receptors (i.e., inside-out signaling); however, no alternative functional significance has been proposed. Our study focused on how changes in the phosphorylation state of beta 2-integrin-rece...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hellberg C,Eierman D,Sjölander A,Andersson T

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  • Cell cycle-dependent DNA damage signaling induced by ICRF-193 involves ATM, ATR, CHK2, and BRCA1.

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    journal_title:Experimental cell research

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    authors: Park I,Avraham HK

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    journal_title:Experimental cell research

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    authors: Hedström E,Issaeva N,Enge M,Selivanova G

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    journal_title:Experimental cell research

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    authors: Rico-Bautista E,Negrín-Martínez C,Novoa-Mogollón J,Fernández-Perez L,Flores-Morales A

    更新日期:2004-03-10 00:00:00

  • Basic fibroblast growth factor downregulates Bcl-2 and promotes apoptosis in MCF-7 human breast cancer cells.

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    journal_title:Experimental cell research

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    authors: Grinnell F,Ho CH

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    authors: Atadja P,Wong H,Veillete C,Riabowol K

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    journal_title:Experimental cell research

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    authors: Chen H,Liu Y,Hu Q

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    authors: Maity A,Solomon D

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  • TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brothman AR,Lindell TJ

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sebag SC,Koval OM,Paschke JD,Winters CJ,Comellas AP,Grumbach IM

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