当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Serum effects on the in vitro differentiation of sea urchin micromeres.

Serum effects on the in vitro differentiation of sea urchin micromeres.

Abstract：

:When micromeres isolated from the 16-cell stage of Strongylocentrotus purpuratus are cultured in sea water containing 3.5% horse serum, they produce spicules at approximately the same time as in normal development. The serum requirement of the micromeres has been investigated by adding serum at varying intervals after isolation or by pulsing the cells with serum at specific times during their in vitro development. The optimum time of serum addition for spicule formation is 36 h after fertilization (AF). Further delay in the addition of serum results in a reduction in the number of spicules formed in culture and a delay in the time at which they appear. A 1-h pulse of serum at 36 h AF is sufficient to initiate a response in some of the micromere aggregates. A 12-h pulse at 36 h AF produces the maximum number of spicules per culture. The critical period for serum addition, 36-48 h AF, corresponds to the time in the normal embryo at which the syncytial primary mesenchyme ring is formed. Electron micrographs of cultured cells demonstrate that micromeres cultured without serum until 48 h AF fail to form pseudopodial extensions and remain as rosette-like clusters of cells. If serum is present, extensive pseudopodial networks form which resemble the primary ring syncytium. These results suggest that serum acts to stimulate fused pseudopodial networks in cultures of micromeres and that the resulting syncytium is necessary for spicule formation.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

McCarthy RA,Spiegel M

doi

10.1016/0014-4827(83)90355-5

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

433-41

issue

2

eissn

0014-4827

issn

1090-2422

pii

0014-4827(83)90355-5

journal_volume

149

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Stimulation of oxygen consumption promotes mitochondrial calcium accumulation, a process associated with, and causally linked to, enhanced formation of tert-butylhydroperoxide-induced DNA single-strand breaks.

    abstract：:The NADH-linked substrates pyruvate, L-glutamine, and beta-hydroxybutyrate, while enhancing the rate of oxygen consumption, also increased the formation of DNA single-strand breaks induced by tert-butylhydroperoxide in intact U937 cells. A cause-effect relationship between these two parameters was established by showi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3779

    authors： Guidarelli A,Brambilla L,Clementi E,Sciorati C,Cantoni O

    更新日期：1997-11-25 00:00:00

  • An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1.

    abstract：:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0078

    authors： Okuda A,Kimura G

    更新日期：1996-03-15 00:00:00

  • Secretory organelle docking at the cell membrane of Paramecium cells: dedocking and synchronized redocking of trichocysts.

    abstract：:We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90013-z

    authors： Pape R,Plattner H

    更新日期：1990-12-01 00:00:00

  • Repression of transcription and interference with DNA binding of TATA-binding protein by C-terminal alternatively spliced p53.

    abstract：:The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5596

    authors： Huang H,Kaku S,Knights C,Park B,Clifford J,Kulesz-Martin M

    更新日期：2002-10-01 00:00:00

  • The effect of a 10-day space flight on the function, phenotype, and adhesion molecule expression of splenocytes and lymph node lymphocytes.

    abstract：:Previous studies have indicated that space flight affects the activation of lymphocytes from humans, monkeys, and rodents. In rats, where lymphocytes from blood, spleen, and lymph nodes have been tested, the accumulated data suggest that the effects of flight on various cells are lymphoid organ-specific. Thus, cells m...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1210

    authors： Grove DS,Pishak SA,Mastro AM

    更新日期：1995-07-01 00:00:00

  • The protein composition of the nuclear matrix of murine P19 embryonal carcinoma cells is differentiation-stage dependent.

    abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90072-4

    authors： Stuurman N,Van Driel R,De Jong L,Meijne AM,Van Renswoude J

    更新日期：1989-02-01 00:00:00

  • Cardiac apoptosis caused by elevated cholesterol level in experimental autoimmune myocarditis.

    abstract：:It has been reported that cholesterol-lowing agents can ameliorate severity of myocarditis. However, the beneficial effect of the agents has been claimed to be independent of cholesterol reduction as there is no significant change in the plasma cholesterol level in myocarditis. In the present study, using experimental...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112169

    authors： Chang H,Wang Y,Wu Y,Ma P,Song Y,Liu C,Ye Y,Qi JH,Qi Z

    更新日期：2020-10-01 00:00:00

  • BAG6 is a novel microtubule-binding protein that regulates ciliogenesis by modulating the cell cycle and interacting with γ-tubulin.

    abstract：:Microtubule-binding proteins provide an alternative and vital pathway to the functional diversity of microtubules. Considerable work is still required to understand the complexities of microtubule-associated cellular processes and to identify novel microtubule-binding proteins. In this study, we identify Bcl2-associat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111776

    authors： He X,Zhang Y,Yang L,Feng J,Yang S,Li T,Zhong T,Li Q,Xie W,Liu M,Zhou J,Li D,Xie S

    更新日期：2020-02-01 00:00:00

  • Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology.

    abstract：:We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled lo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0223

    authors： Goldmann WH,Ezzell RM

    更新日期：1996-07-10 00:00:00

  • The antioxidant, rather than prooxidant, activities of quercetin on normal cells: quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis.

    abstract：:The bioflavonoid quercetin is a dietary anticancer chemical that is capable of inducing apoptosis in tumor cells. Although the activity of quercetin is believed to be due to its antioxidative properties, it has recently been suggested that quercetin also has prooxidant activities, which might effect cytotoxicity direc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00410-5

    authors： Lee JC,Kim J,Park JK,Chung GH,Jang YS

    更新日期：2003-12-10 00:00:00

  • Protective effect of N-acetylcysteine in tumor necrosis factor-alpha-induced apoptosis in U937 cells: the role of mitochondria.

    abstract：:The existence of two different pathways for cell death has been postulated. In addition to the passive and traumatic process leading to necrosis, an active program characterized by organelle integrity and called apoptosis has been described. A positive correlation between the apoptotic cell death process and oxidative...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1311

    authors： Cossarizza A,Franceschi C,Monti D,Salvioli S,Bellesia E,Rivabene R,Biondo L,Rainaldi G,Tinari A,Malorni W

    更新日期：1995-09-01 00:00:00

  • CDK8 regulates the angiogenesis of pancreatic cancer cells in part via the CDK8-β-catenin-KLF2 signal axis.

    abstract：BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angioge...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.05.033

    authors： Wei R,Kong L,Xiao Y,Yuan H,Song Y,Wang J,Yu H,Mao S,Xu W

    更新日期：2018-08-15 00:00:00

  • TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.

    abstract：:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.007

    authors： Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

    更新日期：2011-07-15 00:00:00

  • Regulation of angiogenesis by PI3K signaling networks.

    abstract：:Phosphoinositide 3-kinases (PI3Ks) are an evolutionary conserved family of lipid kinases that control cell growth, metabolism and survival. By generating lipid second messengers that interact with specialized lipid-binding domains found in a wide spectrum of signaling molecules, PI3Ks instigate signaling through a net...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2013.02.021

    authors： Graupera M,Potente M

    更新日期：2013-05-15 00:00:00

  • Use of nonyl acridine orange and rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle.

    abstract：:Specific mitochondrial incorporation of 10 N-nonyl acridine orange (NAO) is demonstrated by subcellular fractionation of rat hepatocytes. Moreover, comparative studies with NAO and rhodamine 123 (Rh 123) prove that acridine orange-derivative uptake is independent of transmembrane mitochondrial potential, a property al...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90219-z

    authors： Leprat P,Ratinaud MH,Maftah A,Petit JM,Julien R

    更新日期：1990-01-01 00:00:00

  • Selective proteolysis of the nuclear replication factor MCM3 in apoptosis.

    abstract：:Cleavage of specific protein subsets is a key event in the execution of apoptosis. Protein degradation may serve for the structural alterations that result in cell self-destruction, but it may also function as a switch in the decisions between apoptosis and necrosis or apoptosis and cell proliferation. Here, we show t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3850

    authors： Schwab BL,Leist M,Knippers R,Nicotera P

    更新日期：1998-02-01 00:00:00

  • The content of DNA and RNA in microparticles released by Jurkat and HL-60 cells undergoing in vitro apoptosis.

    abstract：:Microparticles are small membrane-bound vesicles that are released from apoptotic cells during blebbing. These particles contain DNA and RNA and display important functional activities, including immune system activation. Furthermore, nucleic acids inside the particle can be analyzed as biomarkers in a variety of dise...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.12.014

    authors： Reich CF 3rd,Pisetsky DS

    更新日期：2009-03-10 00:00:00

  • Erythropoietin-dependent autocrine secretion of tumor necrosis factor-alpha in hematopoietic cells modulates proliferation via MAP kinase--ERK-1/2 and does not require tyrosine docking sites in the EPO receptor.

    abstract：:Primary erythroid cells and erythroid cell lines may synthesize and secrete tumor necrosis factor-alpha (TNF-alpha) following stimulation with erythropoietin (EPO). The effect of triggering TNF-alpha synthesis and secretion was investigated in erythroleukemia and myeloid cell lines: HCD57, DA3-EPOR, and BAF3-EPOR. The...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.04.009

    authors： Chen J,Jacobs-Helber SM,Barber DL,Sawyer ST

    更新日期：2004-08-01 00:00:00

  • FTO promotes cell proliferation and migration in esophageal squamous cell carcinoma through up-regulation of MMP13.

    abstract：:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and West...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.111894

    authors： Liu S,Huang M,Chen Z,Chen J,Chao Q,Yin X,Quan M

    更新日期：2020-04-01 00:00:00

  • Growth stimulation by retinol apparently independent of EGF receptor.

    abstract：:A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90578-1

    authors： Böning W,Kakunaga T,Emura M,Mohr U

    更新日期：1986-03-01 00:00:00

  • Isolation of a G0-specific ts mutant from a Fischer rat cell line, 3Y1.

    abstract：:A ts mutant clone, tsJT60, was isolated from Fisher rat cell line, 3Y1. During the exponential growth at both 34 and 39.5 degrees C, tsJT60 did not appear as ts mutant cells. However, once entered resting state (G0) under serum deprivation at the confluent state, they could re-enter S phase at 34 degrees C but could n...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90701-8

    authors： Ide T,Ninomiya J,Ishibashi S

    更新日期：1984-01-01 00:00:00

  • Reconstitution of dna synthetic capacity in senescent normal human fibroblasts by expressing cellular factors E2F and Mdm2.

    abstract：:Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenoviru...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5352

    authors： Sarraj S,Farb R,Martell RE

    更新日期：2001-11-01 00:00:00

  • The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells.

    abstract：:The Drosophila sponge (spg)/CG31048 gene belongs to the dedicator of cytokinesis (DOCK) family genes that are conserved in a wide variety of species. DOCK family members are known as DOCK1-DOCK11 in mammals. Although DOCK1 and DOCK2 involve neurite elongation and immunocyte differentiation, respectively, the functions...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.024

    authors： Eguchi K,Yoshioka Y,Yoshida H,Morishita K,Miyata S,Hiai H,Yamaguchi M

    更新日期：2013-08-15 00:00:00

  • Molecular functions of brain expressed X-linked 2 (BEX2) in malignancies.

    abstract：:Over the last decade there has been growing evidence that Brain Expressed X-Linked 2 (BEX2) has a significant role in the process of carcinogenesis. Collectively, available studies suggest a pro-oncogenic function for this gene in multiple malignancies, including breast, colorectal and hepatocellular cancers in additi...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2019.02.014

    authors： Naderi A

    更新日期：2019-03-15 00:00:00

  • Human calmodulin-like protein is an epithelial-specific protein regulated during keratinocyte differentiation.

    abstract：:Human calmodulin-like protein (CLP) is a calcium-binding protein down-regulated in a cell culture model of mammary tumorigenesis as well as in a majority of breast cancers in vivo. CLP down-regulation may be a result of the poorly differentiated state of these cell lines and tumors, or CLP expression may be incompatib...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5254

    authors： Rogers MS,Kobayashi T,Pittelkow MR,Strehler EE

    更新日期：2001-07-15 00:00:00

  • Diacytosis of human asialotransferrin type 3 in the rat liver is due to the sequential engagement of two receptors.

    abstract：:The possible role of transferrin receptors in the diacytosis of human asialotransferrin type 3 (HAsTf-3) by the rat liver was studied in vivo. A trace dose of the ligand was allowed to compete for hepatic binding sites against diferric transferrin, the concentration of which was varied between 5 400- and 18 000-fold. ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90230-7

    authors： Regoeczi E,Koj A

    更新日期：1985-09-01 00:00:00

  • Neurite extension by neuroblastoma cells on substratum-bound fibronectin's cell-binding fragment but not on the heparan sulfate-binding protein, platelet factor-4.

    abstract：:Human and rat neuroblastoma cells extend neurites over plasma fibronectin (pFN)-coated substrata. For resolution of which fibronectin binding activities (the cell-binding domain (CBD), the heparan sulfate-binding domains, or a combination of the two) are responsible for neurite outgrowth, CBD was prepared free of hepa...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90464-1

    authors： Tobey SL,McClelland KJ,Culp LA

    更新日期：1985-06-01 00:00:00

  • Cellular localization and expression of template-activating factor I in different cell types.

    abstract：:Template-activating factors I (TAF-I) alpha and beta have been identified as chromatin remodeling factors from human HeLa cells. TAF-I beta corresponds to the protein encoded by the set gene, which was found in an acute undifferentiated leukemia as a fusion version with the can gene via chromosomal translocation. To d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3930

    authors： Nagata K,Saito S,Okuwaki M,Kawase H,Furuya A,Kusano A,Hanai N,Okuda A,Kikuchi A

    更新日期：1998-05-01 00:00:00

  • Production of trypsin by cells of the exocrine pancreas is paralleled by the expression of the KH protein vigilin.

    abstract：:Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3891

    authors： Kruse C,Emmrich J,Rumpel E,Klinger MH,Grünweller A,Rohwedel J,Krammer HJ,Kühnel W,Müller PK

    更新日期：1998-02-25 00:00:00

  • AKT upregulates B-Raf Ser445 phosphorylation and ERK1/2 activation in prostate cancer cells in response to androgen depletion.

    abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.008

    authors： Hong SK,Jeong JH,Chan AM,Park JI

    更新日期：2013-07-15 00:00:00