Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.

abstract：:CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...

journal_title：Experimental cell research

authors： Chen CJ,Nguyen T,Shively JE

更新日期：2010-02-15 00:00:00

abstract：:dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and is the central regulator of cellular dUTP pools. Nuclear (DUT-N) and mitochondrial (DUT-M) isoforms of the protein have been identified in humans and arise from the same gene by the alternative use of 5' exons. Recently, ...

journal_title：Experimental cell research

authors： Tinkelenberg BA,Fazzone W,Lynch FJ,Ladner RD

更新日期：2003-07-01 00:00:00

abstract：:Liver cells isolated from the adult rat livers under mild conditions were preincubated for 1 day with Williams medium E (WE) containing serum, dexamethasone and insulin, and then the cells (monolayered) were incubated for 2-3 days with WE (1 ml) containing only insulin to measure DNA synthesis and/or mitosis. DNA synt...

journal_title：Experimental cell research

authors： Koji T,Terayama H

更新日期：1984-12-01 00:00:00

abstract：:Tpr is a 267-kDa protein of unknown function recently identified as a constitutive component of the nuclear pore complex (NPC2)-attached intranuclear filaments. Secondary structure predictions suggest that the protein is divided into a large, coiled-coil forming aminoterminal domain and a shorter, highly acidic carbox...

journal_title：Experimental cell research

authors： Cordes VC,Hase ME,Müller L

更新日期：1998-11-25 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：:A polypeptide growth factor has been isolated from serum-free medium conditioned by mouse PC13 embryonal carcinoma cells, which is strongly mitogenic for Swiss 3T3 fibroblasts. On a CM-2-SW high-performance liquid chromatography cation-exchange column at low pH, this growth factor elutes at a salt concentration very c...

journal_title：Experimental cell research

authors： van Veggel JH,van Oostwaard TM,de Laat SW,van Zoelen EJ

更新日期：1987-04-01 00:00:00

abstract：:Human endothelial cells, when suspended within three-dimensional collagen matrices, develop intracellular vacuoles that coalesce to form capillary lumens and tubes. Vacuole and lumen formation are completely dependent on the collagen-binding integrin alpha 2 beta 1, while other endothelial cell integrins had no appare...

journal_title：Experimental cell research

authors： Davis GE,Camarillo CW

更新日期：1996-04-10 00:00:00

abstract：:Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...

journal_title：Experimental cell research

authors： Zaricznyj C,Macara IG

更新日期：1987-02-01 00:00:00

abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

journal_title：Experimental cell research

authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

更新日期：2000-08-01 00:00:00

abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...

journal_title：Experimental cell research

authors： Corbett A,Ferrigno P,Henry M,Kahana J,Koepp D,Lee M,Nguyen L,Schlenstedt G,Seedorf M,Shen E,Taura T,Wong D,Silver P

更新日期：1996-12-15 00:00:00

abstract：:Statins are competitive inhibitors of hydroxy-methyl-glutaryl Coenzyme A (HMG-CoA) reductase, a key enzyme involved in the conversion of HMG-CoA to the cholesterol precursor mevalonate. Some statins, such as simvastatin (simvastatin), have been shown to have anti-cancer and anti-inflammatory effects, reducing cartilag...

journal_title：Experimental cell research

authors： Han Y,Kim SJ

更新日期：2016-08-15 00:00:00

abstract：:Previously, whole-mount electron microscopy of nuclei extruded together with residual cytoplasm from the rhizoids of several algal species of the order Dasycladales has revealed the occurrence of an intra- and perinuclear network of 10-nm filaments morphologically indistinguishable from that of mammalian vimentin inte...

journal_title：Experimental cell research

authors： Berger S,Wittke W,Traub P

更新日期：1998-05-01 00:00:00

abstract：:Pancreatic cancer is one of the most malignant tumors. Invasion and metastasis can occur in the early stage of pancreatic cancer, contributing to the poor prognosis. Accordingly, in this study, we evaluated the molecular mechanisms underlying invasion and metastasis. Using mass spectrometry, we found that Integrin alp...

journal_title：Experimental cell research

authors： Wu Y,Tan X,Liu P,Yang Y,Huang Y,Liu X,Meng X,Yu B,Wu M,Jin H

更新日期：2019-06-01 00:00:00

abstract：:Plasmid 6.4 kbp DNA, 14 kbp DNA, lambda phage particles, all of which contained herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, or IgM molecules, were mixed with erythrocyte membranes and treated with neutral detergent. The transparent mixture was diluted with phosphate-buffered saline (PBS), followed ...

journal_title：Experimental cell research

authors： Sugawa H,Uchida T,Yoneda Y,Ishiura M,Okada Y

更新日期：1985-08-01 00:00:00

abstract：:Ring finger protein 6 (RNF6), a RING-domain E3 ubiquitin Ligase, functions as a potential tumor promoter in several caners. However, the function and clinical significance of RNF6 in hepatocellular carcinoma (HCC) remains unclear. Here, we found that high RNF6 expression was detected frequently in primary HCC tissues,...

journal_title：Experimental cell research

authors： Cai J,Xiong Q,Jiang X,Zhou S,Liu T

更新日期：2019-01-01 00:00:00

abstract：:The relationship between topoisomerase II activity and ribosomal RNA synthesis was investigated using the antitumoral drug VM26, a specific inhibitor of topoisomerase II. For this purpose TG cells, a human tumor cell line, were cultured in the presence of 2.5 microM VM26 for 1 and 3 h; VM26 reduced the topoisomerase I...

journal_title：Experimental cell research

authors： Govoni M,Farabegoli F,Pession A,Novello F

更新日期：1994-03-01 00:00:00

abstract：:Prosomes constitute the multicatalytic proteinase (MCP) core of the 26S proteasomes, but were first observed as subcomplexes of untranslated mRNP; this suggests that they play a putative role in the control of protein biosynthesis in addition to their catabolic enzymatic function. In previous investigations it was sho...

journal_title：Experimental cell research

authors： De Conto F,Missorini S,Arcangeletti C,Pinardi F,Montarras D,Pinset C,Vassy J,Géraud G,Chezzi C,Scherrer K

更新日期：1997-05-25 00:00:00

abstract：:The aim of this study was to assess the effect of different culture conditions on the survival and morphological phenotype of cultured acinar cells. Acinar fragments isolated from hamster pancreas were embedded in rat-tail collagen. Four groups were established: Medium 1-5% NuSerum + basic medium (basic medium = DMEM/...

journal_title：Experimental cell research

authors： Yuan S,Duguid WP,Agapitos D,Wyllie B,Rosenberg L

更新日期：1997-12-15 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD), a hereditary renal disease caused by mutations in PKD1 (85%) or PKD2 (15%), is characterized by the development of gradually enlarging multiple renal cysts and progressive renal failure. Polycystin-1 (PC1), PKD1 gene product, is an integral membrane glycoprotein wh...

journal_title：Experimental cell research

authors： Kim H,Kang AY,Ko AR,Park HC,So I,Park JH,Cheong HI,Hwang YH,Ahn C

更新日期：2014-01-01 00:00:00

abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

journal_title：Experimental cell research

authors： Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

更新日期：2004-07-15 00:00:00

abstract：:The mitochondrial enzyme cytochrome c oxidase (COX) in eukaryotes consists of at least seven subunits, three of which (I-III) are encoded by mitochondrial DNA (mitDNA) and the others (IV-VII) by the nuclear genome. There is increasing evidence that COX in mammals exists in multiple tissue-specific forms, presumably sp...

journal_title：Experimental cell research

authors： Nakagawa M,Miranda AF

更新日期：1987-01-01 00:00:00

abstract：:Gastric cancer tissue-derived mesenchymal stem cells (GC-MSCs) are important resident stromal cells in the tumor microenvironment (TME) and have been shown to play a key role in gastric cancer progression. Whether GC-MSCs exert a tumor-promoting function by affecting anti-tumor immunity is still unclear. In this study...

journal_title：Experimental cell research

authors： Wang M,Chen B,Sun XX,Zhao XD,Zhao YY,Sun L,Xu CG,Shen B,Su ZL,Xu WR,Zhu W

更新日期：2017-12-01 00:00:00

abstract：:Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether a SUV39H1-low chromatin state promotes migratory cell populations in cervical cancers, using meta-analysis of data f...

journal_title：Experimental cell research

authors： Rodrigues C,Pattabiraman C,Vijaykumar A,Arora R,Narayana SM,Kumar RV,Notani D,Varga-Weisz P,Krishna S

更新日期：2019-05-15 00:00:00

abstract：:Excision of chromatin loop domains and internucleosomal DNA fragmentation are widely considered as consecutive stages of chromatin disassembly during apoptosis. We report here on apoptosis induced by staurosporine in NB-2a neuroblastoma cells, which was accompanied by excision of chromatin loop domains, but proceeded ...

journal_title：Experimental cell research

authors： Solovyan VT

更新日期：2007-04-15 00:00:00

abstract：:The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

journal_title：Experimental cell research

authors： Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

更新日期：2005-08-01 00:00:00

abstract：:It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

journal_title：Experimental cell research

authors： Hao J,Hu Y,Li Y,Zhou Q,Lv X

更新日期：2017-08-15 00:00:00

abstract：:Identification of breast cancer not being a single disease but backed by multiple heterogeneous oncogenic subpopulations is of growing interest in developing personalized therapies to provide optimal outcomes. Through this review, we bring attention to evolution of tumor and microenvironment heterogeneity as a predomi...

journal_title：Experimental cell research

authors： Nandy A,Gangopadhyay S,Mukhopadhyay A

更新日期：2014-01-01 00:00:00

abstract：:Phosphorylation of types III and IV intermediate filaments (IFs) is known to regulate their organization and function. Phosphorylation of the amino-terminal head domain sites on types III and IV IF proteins plays a key role in the assembly/disassembly of IF subunits into 10 nm filaments, and influences the phosphoryla...

journal_title：Experimental cell research

authors： Sihag RK,Inagaki M,Yamaguchi T,Shea TB,Pant HC

更新日期：2007-06-10 00:00:00

abstract：:We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...

journal_title：Experimental cell research

authors： Pape R,Plattner H

更新日期：1990-12-01 00:00:00

abstract：:The Ag-NOR proteins are defined as markers of "active" ribosomal genes. They correspond to a set of proteins specifically located in the nucleolar organizer regions (NORs), but have not yet been clearly identified. We adapted the specific detection method of the Ag-NOR proteins to Western blots in order to identify th...

journal_title：Experimental cell research

authors： Roussel P,Belenguer P,Amalric F,Hernandez-Verdun D

更新日期：1992-11-01 00:00:00