Inhibition of topoisomerase II activity and its effect on nucleolar structure and function.

abstract：:Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

journal_title：Experimental cell research

authors： Suzuki ST

更新日期：2000-11-25 00:00:00

abstract：:Allograft Inflammatory Factor-1 (AIF-1) is a cytoplasmic calcium-binding protein expressed in vascular smooth muscle cells (VSMC) in response to injury or cytokine stimulation. AIF-1 contains a partially conserved EF-hand calcium-binding domain, and participates in VSMC activation by activation of Rac1 and induction o...

journal_title：Experimental cell research

authors： Autieri MV,Chen X

更新日期：2005-07-01 00:00:00

abstract：:The CDC25Mm gene codes for Ras-guanine nucleotide exchange factors. Four different full-length cDNA clones derived from the same gene and coding for proteins of different sizes that have in common the last 661 amino acids have been isolated from mouse brain. In order to investigate the expression of the products of th...

journal_title：Experimental cell research

authors： Ferrari C,Zippel R,Martegani E,Gnesutta N,Carrera V,Sturani E

更新日期：1994-02-01 00:00:00

abstract：:We have investigated the role played by the growth and differentiation factor (GDF)-induced calcium ion second messenger signal in the control of cellular differentiation and proliferation in Syrian hamster embryo (SHE) cells. Blocking the platelet-derived growth factor (PDGF)-induced calcium ion signal with either ex...

journal_title：Experimental cell research

authors： Isfort RJ,Cody DB,Stuard SB,Ridder GM,LeBoeuf RA

更新日期：1996-08-01 00:00:00

abstract：:A cell culture model stimulating the genetic deficiency of glucocerebrosidase has been developed, utilizing macrophages and conduritol B epoxide (CBE), the specific irreversible inhibitor of the enzyme. Rat peritoneal macrophage glucocerebrosidase was completely inhibited when cells were treated with 10 microM CBE for...

journal_title：Experimental cell research

authors： Das PK,Murray GJ,Gal AE,Barranger JA

更新日期：1987-02-01 00:00:00

abstract：:The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleola...

journal_title：Experimental cell research

authors： Vandelaer M,Thiry M,Goessens G

更新日期：1993-04-01 00:00:00

abstract：:We have earlier shown that mouse pancreatic acinar cells produce hydrogen sulfide (H(2)S), which plays a key role in the pathogenesis of acute pancreatitis (AP). H(2)S-dependent induction of inflammation is mediated by the activation of transcription factor NF-kappaB. We now provide evidence that activation of Src fam...

journal_title：Experimental cell research

authors： Tamizhselvi R,Koh YH,Sun J,Zhang H,Bhatia M

更新日期：2010-05-15 00:00:00

abstract：:Microtubule-binding proteins provide an alternative and vital pathway to the functional diversity of microtubules. Considerable work is still required to understand the complexities of microtubule-associated cellular processes and to identify novel microtubule-binding proteins. In this study, we identify Bcl2-associat...

journal_title：Experimental cell research

authors： He X,Zhang Y,Yang L,Feng J,Yang S,Li T,Zhong T,Li Q,Xie W,Liu M,Zhou J,Li D,Xie S

更新日期：2020-02-01 00:00:00

abstract：:The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave...

journal_title：Experimental cell research

authors： Kitajima Y,Owaribe K,Nishizawa Y,Jokura Y,Yaoita H

更新日期：1992-11-01 00:00:00

abstract：:The highly purified, luminal domain of rat lung endothelial plasma membranes was used as an immunogen to obtain monoclonal antibodies to the endothelial cell surface. The procedure was highly efficient, yielding antibodies which recognize three seemingly novel endothelial integral membrane glycoproteins of molecular w...

journal_title：Experimental cell research

authors： Ghitescu LD,Crine P,Jacobson BS

更新日期：1997-04-10 00:00:00

abstract：:The J.774 murine macrophage cells were cultured in suspension in Teflon flasks. When allowed to attach on culture plastic dishes, a 2-3-fold increase in transferrin binding was observed. This occurred in 10 min, reached a steady state at 60 min, remained stable for several hours and was reversible after resuspension o...

journal_title：Experimental cell research

authors： Ventura MA,Louache F,Rouis M,Erlich D,Goldstein S,Testa U,Thomopoulos P

更新日期：1987-06-01 00:00:00

abstract：:Trophoblast cells were grown on filters that allow access to apical and basal surfaces of cells. Using this experimental system, IgG transport was shown to be specific and to occur primarily in the apical to basal direction. This transport was time- and temperature-dependent, with approximately 10% of added IgG appear...

journal_title：Experimental cell research

authors： Sooranna SR,Contractor SF

更新日期：1991-01-01 00:00:00

abstract：:We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

journal_title：Experimental cell research

authors： Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

更新日期：2016-09-10 00:00:00

abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

journal_title：Experimental cell research

authors： Qian J,Tu R,Yuan L,Xie W

更新日期：2016-06-10 00:00:00

abstract：:Growth factors and cytokines initiate multiple signal transduction pathways that lead to cell survival, cell cycle progression or differentiation. A common feature of these pathways is increased cellular metabolism and glucose uptake. Furthermore, the energy requirements of many cancers and transformed cell lines are ...

journal_title：Experimental cell research

authors： Kansara M,Berridge MV

更新日期：2004-02-15 00:00:00

abstract：:It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

journal_title：Experimental cell research

authors： Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

更新日期：2006-08-01 00:00:00

abstract：:Strategies for correct diagnosis, treatment evaluation and recurrence prediction are important for the prognosis and mortality rates among cancer patients. In spite of major improvements in clinical management, gastrointestinal stromal tumors (GISTs) can still be deadly due to metastasis and recurrences, which confirm...

journal_title：Experimental cell research

authors： Berglund E,Daré E,Branca RM,Akcakaya P,Fröbom R,Berggren PO,Lui WO,Larsson C,Zedenius J,Orre L,Lehtiö J,Kim J,Bränström R

更新日期：2015-08-01 00:00:00

abstract：:Previous morphological and biochemical studies have suggested that actin and actin-containing filaments (microfilaments) exist in the eukaryotic nucleus and that they perform important nuclear functions. However, the concept is not widely accepted. In this study, we demonstrate actin and bundles of actin in the nuclei...

journal_title：Experimental cell research

authors： Parfenov VN,Davis DS,Pochukalina GN,Sample CE,Bugaeva EA,Murti KG

更新日期：1995-04-01 00:00:00

abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

journal_title：Experimental cell research

authors： Myrdal SE,Auersperg N

更新日期：1985-08-01 00:00:00

abstract：:Considering that stimulation of melanogenesis may lead to alterations of cellular responses, besides melanin production, our main goal was to study the cellular effects of melanogenesis stimulation of B16-F10 melanoma cells. Our results show increased levels of the reactive oxygen species after 15 h of melanogenesis s...

journal_title：Experimental cell research

authors： Cunha ES,Kawahara R,Kadowaki MK,Amstalden HG,Noleto GR,Cadena SM,Winnischofer SM,Martinez GR

更新日期：2012-09-10 00:00:00

abstract：:A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...

journal_title：Experimental cell research

authors： Nishiyama I,Fujii T

更新日期：1992-02-01 00:00:00

abstract：:Under standard culture conditions, normal human mammary epithelial cells (HMECs) divide a limited number of times before proliferation ceases in a growth-arrested state referred to as selection. Cells that have undergone spontaneous loss of p16(INK4a) expression due to hypermethylation of the p16(INK4a) CpG island eme...

journal_title：Experimental cell research

authors： Huschtscha LI,Neumann AA,Noble JR,Reddel RR

更新日期：2001-04-15 00:00:00

abstract：:When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta and vitamin D can influence this process and can stimulate chondrogenesis. Given the ...

journal_title：Experimental cell research

authors： Tsonis PA,Del Rio-Tsonis K,Millan JL,Wheelock MJ

更新日期：1994-08-01 00:00:00

abstract：:Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...

journal_title：Experimental cell research

authors： Hinz B,McCulloch CA,Coelho NM

更新日期：2019-06-01 00:00:00

abstract：:TGFβ contributes to mesangial cell hypertrophy and matrix protein increase in various kidney diseases including diabetic nephropathy. Deptor is an mTOR-interacting protein and suppresses mTORC1 and mTORC2 activities. We have recently shown that TGFβ-induced inhibition of deptor increases the mTOR activity. The mechani...

journal_title：Experimental cell research

authors： Maity S,Bera A,Ghosh-Choudhury N,Das F,Kasinath BS,Choudhury GG

更新日期：2018-03-01 00:00:00

abstract：:Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

journal_title：Experimental cell research

authors： Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

更新日期：2015-02-15 00:00:00

abstract：PURPOSE:Owing to the late diagnosis and frequent metastasis, ovarian cancer (OC) exhibits a high mortality rate. The study was intended to figure out the function of long non-coding RNA (lncRNA) HCP5 in OC metastasis. METHODS:Microarray analysis was conducted to probe aberrantly expressed lncRNAs in OC tissues. Artifi...

journal_title：Experimental cell research

authors： Wang L,He M,Fu L,Jin Y

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:CDK8 is associated with the transcriptional Mediator complex and has been shown to regulate several transcription factors implicated in cancer. As a pancreatic cancer oncogene, the role of CDK8 in cancer angiogenesis remains unclear. Here, we investigated the contribution of CDK8 in pancreatic cancer angioge...

journal_title：Experimental cell research

authors： Wei R,Kong L,Xiao Y,Yuan H,Song Y,Wang J,Yu H,Mao S,Xu W

更新日期：2018-08-15 00:00:00

abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...

journal_title：Experimental cell research

authors： Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C

更新日期：1996-03-15 00:00:00

abstract：:It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

journal_title：Experimental cell research

authors： Hao J,Hu Y,Li Y,Zhou Q,Lv X

更新日期：2017-08-15 00:00:00