Abstract：
:The J.774 murine macrophage cells were cultured in suspension in Teflon flasks. When allowed to attach on culture plastic dishes, a 2-3-fold increase in transferrin binding was observed. This occurred in 10 min, reached a steady state at 60 min, remained stable for several hours and was reversible after resuspension of the cells at 37 degrees C. The phenomenon was not dependent on the synthesis of new protein. An opposite change of acetyl LDL receptors was observed, with a threefold decrease of the binding 1 h after the attachment of the cells. The increase of transferrin binding affected almost equally the cell surface and the intracellular sites; therefore it could not be related to a simple shift between these two compartments. It is suggested that the attachment of the cells induced a recruitment of binding sites from a 'silent' pool of receptors. Serum factors, as well as phorbol esters and db-cAMP potentiated the effect of anchorage.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Ventura MA,Louache F,Rouis M,Erlich D,Goldstein S,Testa U,Thomopoulos Pdoi
10.1016/0014-4827(87)90307-7subject
Has Abstractpub_date
1987-06-01 00:00:00pages
290-9issue
2eissn
0014-4827issn
1090-2422journal_volume
170pub_type
杂志文章abstract：:Senescent human diploid fibroblasts, TIG-1, had labelling indices of about 0.5-3% when labelled with [3H]thymidine for 3 days in fresh medium containing 10% fetal bovine serum. When these cells were infected with SV40, the percentage of nuclei incorporating [3H]thymidine increased by about 10-fold. The frequency of T-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90450-0
更新日期：1983-03-01 00:00:00
abstract：:BMPs and Hox proteins play crucial roles in developmental processes. Beyond their mutual regulation of gene expression, little is known about the relations between their mechanisms of actions. Previously, we have shown that Hoxc8 acts as a downstream repressor in the BMP signaling pathway. Smad1 and Smad6 interact wit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.002
更新日期：2006-04-01 00:00:00
abstract：:Interleukin-1 (IL-1) is one of the most potent bone-resorbing factors involved in bone loss associated with inflammation. We previously reported that IL-1 prolonged the survival of multinucleated osteoclast-like cells (OCLs) formed in cocultures of murine osteoblasts/stromal cells and bone marrow cells via the prevent...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4320
更新日期：1999-02-25 00:00:00
abstract：:Mutation R453W in A-type lamins, that are major nuclear envelope proteins, generates Emery-Dreifuss muscular dystrophy. We previously showed that mouse myoblasts expressing R453W-lamin A incompletely exit the cell cycle and differentiate into myocytes with a low level of multinucleation. Here we attempted to improve d...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.01.018
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abstract：:Leptomycin B (LMB) is a Streptomyces metabolite that inhibits nuclear export of the human immunodeficiency virus type 1 regulatory protein Rev at low nanomolar concentrations. Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). Here we show evidence that LMB binds d...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4136
更新日期：1998-08-01 00:00:00
abstract：:The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.02.028
更新日期：2014-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3630
更新日期：1997-08-25 00:00:00
abstract：:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and West...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111894
更新日期：2020-04-01 00:00:00
abstract：:Caveolin-1 (Cav-1) is a principal structural protein of caveolae. Cav-1 has been implicated in cancer progression, but its precise functional roles in pituitary adenoma cells remain largely unclear. In this study, we evidenced that the level of cav-1 was elevated in the invasive pituitary adenoma. Cav-1 knockdown rest...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.01.008
更新日期：2018-06-01 00:00:00
abstract：:Type VI collagen is a nonfibrillar collagen present as a network throughout the chick secondary stroma. Immunolocalization of type VI collagen both in the chick corneal stroma and in other systems demonstrates that type VI collagen is present associated with cells and between striated fibrils. We hypothesize that type...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90200-r
更新日期：1992-06-01 00:00:00
abstract：:It is generally accepted that Friedreich's ataxia (FRDA) is caused by a deficiency in frataxin expression, a mitochondrial protein involved in iron homeostasis, which mainly affects the brain, dorsal root ganglia of the spinal cord, heart and in certain cases the pancreas. However, there is little knowledge as to othe...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.04.013
更新日期：2015-07-01 00:00:00
abstract：:The role of glycans in the apical targeting of proteins in epithelial cells remains a debated question. We have expressed the mouse soluble dipeptidyl peptidase IV (DPP IV ectodomain) in kidney (MDCK) and in intestinal (Caco-2) epithelial cell lines, as a model to study the role of glycosylation in apical targeting. T...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4894
更新日期：2000-07-10 00:00:00
abstract：:Replication protein A (RPA), a stable complex of three polypeptides, is the single-stranded DNA-binding protein essential for DNA replication in eukaryotic cells. Previous studies of the subcellular distribution and stability of the RPA heterotrimer during the mammalian cell cycle have produced conflicting results. He...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4770
更新日期：2000-02-01 00:00:00
abstract：:T cell polarization and redistribution of cellular components are critical to processes such as activation, migration, and potentially HIV infection. Here, we investigate the effects of CD4 engagement on the redistribution and localization of chemokine receptors, CXCR4 and CCR5, adhesion molecules, and lipid raft comp...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.11.022
更新日期：2005-04-01 00:00:00
abstract：:Multipotent mesenchymal stromal cells (MSCs) support wound healing processes. These cells express toll-like receptors (TLRs). TLRs perform important key functions when the immune system is confronted with danger signals. TLR ligation by lipopolysaccharides (LPS) activates MSCs and induces intracellular signaling casca...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.07.030
更新日期：2018-09-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(05)80067-9
更新日期：1992-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4899
更新日期：2000-06-15 00:00:00
abstract：:Liver endothelium has been reported to possess membrane receptors for the iron-binding protein transferrin (Tf). Similarly, the core protein of proteoglycans (PG) associated with cell membrane in many cell systems can bind Tf. To find out if membrane-associated proteoglycans can explain Tf-binding ability of liver end...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90120-y
更新日期：1990-03-01 00:00:00
abstract：:The formation of functional gap junctions between astrocytes was investigated during differentiation of these cells in culture. Precursor cells of GFA (glial fibrillary acidic) protein-positive astrocytes were cultured in a chemically defined medium as a homogeneous population. These cells were rarely coupled to one n...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(85)80001-x
更新日期：1985-08-01 00:00:00
abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.08.010
更新日期：2011-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.111746
更新日期：2020-01-15 00:00:00
abstract：:The interphase nucleus is a structurally ordered, three-dimensional structure, in which specific chromatin domains occupy distinct spatial positions that can, in turn, be modified with changes in cell function. A fundamental goal in developmental neurobiology is the identification of mechanisms that dictate the orderl...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4793
更新日期：2000-04-10 00:00:00
abstract：:We have evaluated the importance of the Ser/Thr protein phosphorylation and dephosphorylation for chondrogenesis in high-density chicken limb bud mesenchymal cell cultures (HDCs) by using H89, a cell-permeable protein kinase inhibitor, and okadaic acid (OA), a phosphoprotein phosphatase (PP)-specific inhibitor molecul...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5487
更新日期：2002-04-15 00:00:00
abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.03.002
更新日期：2013-05-01 00:00:00
abstract：:The interaction between the central cell-binding domain (CBD) of fibronectin (FN) and its receptor integrin alpha 5 beta 1 was analyzed by both ligand-binding and cell adhesion assays. The ligands used were a CBD fragment (pCBD) of human plasma fibronectin and its recombinant versions including wild type CBD (wtCBD) a...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1033
更新日期：1995-01-01 00:00:00
abstract：:Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.07.014
更新日期：2009-10-15 00:00:00
abstract：:The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00386-0
更新日期：2003-11-15 00:00:00
abstract：:The MItf-Tfe family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors encodes four family members: MItf, Tfe3, TfeB and TfeC. In vitro, each protein of the family binds DNA in a homo- or heterodimeric form with other family members. Tfe3 is involved in chromosomal translocations recurrent in di...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.09.015
更新日期：2006-12-10 00:00:00
abstract：:Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2013.04.009
更新日期：2013-09-10 00:00:00
abstract：:The ccl mutation in Paramecium tetraurelia reversibly and rapidly blocks cell cycle progression and DNA synthesis at the restrictive temperature. Progression through the cell cycle is blocked during both the G1 and S portions of the cell cycle, while at the restrictive temperature there is neither residual cell cycle ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(84)90220-9
更新日期：1984-12-01 00:00:00
