Abstract:
:Considering that stimulation of melanogenesis may lead to alterations of cellular responses, besides melanin production, our main goal was to study the cellular effects of melanogenesis stimulation of B16-F10 melanoma cells. Our results show increased levels of the reactive oxygen species after 15 h of melanogenesis stimulation. Following 48 h of melanogenesis stimulation, proliferation was inhibited (by induction of cell cycle arrest in the G1 phase) and the expression levels of p21 mRNA were increased. In addition, melanogenesis stimulation did not induce cellular senescence. Proteomic analysis demonstrated the involvement of proteins from other pathways besides those related to the cell cycle, including protein disulfide isomerase A3, heat-shock protein 70, and fructose biphosphate aldolase A (all up-regulated), and lactate dehydrogenase (down-regulated). In RT-qPCR experiments, the levels of pyruvate kinase M2 mRNA dropped, whereas the levels of ATP synthase (beta-F1) mRNA increased. These data indicate that melanogenesis stimulation of B16-F10 cells leads to alterations in metabolism and cell cycle progression that may contribute to an induction of cell quiescence, which may provide a mechanism of resistance against cellular injury promoted by melanin synthesis.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Cunha ES,Kawahara R,Kadowaki MK,Amstalden HG,Noleto GR,Cadena SM,Winnischofer SM,Martinez GRdoi
10.1016/j.yexcr.2012.05.019subject
Has Abstractpub_date
2012-09-10 00:00:00pages
1913-25issue
15eissn
0014-4827issn
1090-2422pii
S0014-4827(12)00257-1journal_volume
318pub_type
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