Use of nonyl acridine orange and rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle.


:Specific mitochondrial incorporation of 10 N-nonyl acridine orange (NAO) is demonstrated by subcellular fractionation of rat hepatocytes. Moreover, comparative studies with NAO and rhodamine 123 (Rh 123) prove that acridine orange-derivative uptake is independent of transmembrane mitochondrial potential, a property allowing its utilization for the assessment of mitochondrial membrane mass modifications under various physiological states. Using NAO and Rh 123, we have respectively followed the biosynthesis of mitochondrial membrane and its assembly under a functional state during the L1210 cell cycle. Their evolution occurs in two stages according to a well-defined sequential order. Mitochondrial biogenesis, as revealed by NAO incorporation, occurs essentially in the G1 phase (probably mitochondrion enlargement) but also starts in late S phase (probably mitochondrion division). The increased amount of functional mitochondrial membrane, monitored by Rh 123 uptake, is emphasized in late G1 (prerequisite to DNA synthesis) and during G2M phases (prerequisite to mitosis). This alternative succession of phases displays the existence of a time-lag between the biosynthesis of mitochondrial membrane and its functional organization. Such an analysis confirms the potential of the NAO probe to evaluate mitochondrial membrane mass changes in various biological fields.


Exp Cell Res


Leprat P,Ratinaud MH,Maftah A,Petit JM,Julien R




Has Abstract


1990-01-01 00:00:00














  • Identification of avian sarcoplasmic reticulum Ca(2+)-ATPase (SERCA3) as a novel 1,25(OH)(2)D(3) target gene in the monocytic lineage.

    abstract::Osteoclasts are postmitotic, multinucleated giant cells generated by the fusion of hematopoietic mononuclear precursors from the monocyte-macrophage lineage. In culture, adherent macrophages from blood-derived monocytes grow, gather, and fuse together to form multinucleated osteoclast-like cells. These events are cont...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Machuca I,Domenget C,Jurdic P

    更新日期:1999-08-01 00:00:00

  • Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cells in primary culture.

    abstract::Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yamamoto K,Aoyagi M,Yamamoto M

    更新日期:1995-05-01 00:00:00

  • Bone morphogenetic protein-4 is overexpressed in colonic adenocarcinomas and promotes migration and invasion of HCT116 cells.

    abstract::Bone morphogenetic protein (BMP), a member of the TGF-beta superfamily, is involved in development, morphogenesis, cell proliferation and apoptosis. Dysregulation of BMP signaling has been suggested in tumorigenesis. In an analysis of human colon normal mucosa and tumors at different stages by immunohistochemistry, we...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Deng H,Makizumi R,Ravikumar TS,Dong H,Yang W,Yang WL

    更新日期:2007-03-10 00:00:00

  • Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts.

    abstract::The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nöth U,Tuli R,Seghatoleslami R,Howard M,Shah A,Hall DJ,Hickok NJ,Tuan RS

    更新日期:2003-11-15 00:00:00

  • Cancer progression and growth: relationship of paracrine and autocrine growth mechanisms to organ preference of metastasis.

    abstract::The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Nicolson GL

    更新日期:1993-02-01 00:00:00

  • Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation.

    abstract::Identification of transcription factors expressed by differentiated cells is informative not only of tissue-specific pathways, but to help identify master regulators for cellular reprogramming. If applied, such an approach could generate healthy autologous tissue-specific cells for clinical use where cells from the ho...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hustler A,Eardley I,Hinley J,Pearson J,Wezel F,Radvanyi F,Baker SC,Southgate J

    更新日期:2018-08-15 00:00:00

  • Soluble growth factors stimulate spermatogonial stem cell divisions that maintain a stem cell pool and produce progenitors in vitro.

    abstract::Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ebata KT,Yeh JR,Zhang X,Nagano MC

    更新日期:2011-06-10 00:00:00

  • Macrophages enhance tumor-derived autophagosomes (DRibbles)-induced B cells activation by TLR4/MyD88 and CD40/CD40L.

    abstract::Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

    更新日期:2015-02-15 00:00:00

  • Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity.

    abstract::ORP8 is an oxysterol/cholesterol binding protein anchored to the endoplasmic reticulum and the nuclear envelope, and is abundantly expressed in the macrophage. We created and characterized mouse RAW264.7 macrophages with ORP8 stably silenced using shRNA lentiviruses. A microarray transcriptome and gene ontology pathwa...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Béaslas O,Vihervaara T,Li J,Laurila PP,Yan D,Olkkonen VM

    更新日期:2012-09-10 00:00:00

  • Induction of pancreatic cancer cell migration by an autocrine epidermal growth factor receptor activation.

    abstract::Pancreatic cancer is characterized by aggressive local invasion and early metastasis formation. Active migration of the pancreatic cancer cells is essential for these processes. We have shown previously that the pancreatic cancer cells lines CFPAC1 and IMIM-PC2 show high migratory activity, and we have investigated he...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Stock AM,Hahn SA,Troost G,Niggemann B,Zänker KS,Entschladen F

    更新日期:2014-08-15 00:00:00

  • Synchronous protein cycling in batch cultures of the yeast Saccharomyces cerevisiae at log growth phase.

    abstract::The assumption that cells are temporally organized systems, i.e. showing relevant dynamics of their state variables such as gene expression or protein and metabolite concentration, while tacitly given for granted at the molecular level, is not explicitly taken into account when interpreting biological experimental dat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Romagnoli G,Cundari E,Negri R,Crescenzi M,Farina L,Giuliani A,Bianchi MM

    更新日期:2011-12-10 00:00:00

  • Mitochondrial DNA is synthesized during meiosis and spermiogenesis in the mouse.

    abstract::Following intratesticular injection of [3H]thymidine, spermatozoa were isolated from the caput and cauda epididymis of CD-1 mice. Quantitation of the amount of radiolabelled DNA in whole spermatozoa, sperm heads (nuclei) and sperm midpiece-tails (mitochondria) at time intervals 3-36 days postinjection demonstrated mit...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hecht NB,Liem H

    更新日期:1984-09-01 00:00:00

  • Turbidimetric studies on microtubule sliding using the stopped-flow-light-scattering method.

    abstract::The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kamimura S,Nakanishi M,Yano M,Shimizu H

    更新日期:1986-03-01 00:00:00

  • Bone-derived mesenchymal stromal cells from HIV transgenic mice exhibit altered proliferation, differentiation capacity and paracrine functions along with impaired therapeutic potential in kidney injury.

    abstract::Mesenchymal stem cells (MSCs) secrete paracrine factors that could be cytoprotective and serve roles in immunoregulation during tissue injury. Although MSCs express HIV receptors, and co-receptors, and are susceptible to HIV infection, whether HIV-1 may affect biological properties of MSCs needs more study. We evaluat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cheng K,Rai P,Lan X,Plagov A,Malhotra A,Gupta S,Singhal PC

    更新日期:2013-08-15 00:00:00

  • Regulation of pyruvate kinase expression and growth in mastocytoma cells. I. Initial observations.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

    更新日期:1983-12-01 00:00:00

  • Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25.

    abstract::Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

    更新日期:2009-05-01 00:00:00

  • NF-κB mediated miR-130a modulation in lung microvascular cell remodeling: Implication in pulmonary hypertension.

    abstract::Pulmonary arterial hypertension (PAH) is a progressive pulmonary vascular disease which is associated with pulmonary arterial endothelial cells (PAEC) dysfunction and pulmonary arterial smooth muscle cells proliferation. Moreover, inflammation is contributing a critical role in EC dysfunction and remains elusive. Nucl...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li L,Kim IK,Chiasson V,Chatterjee P,Gupta S

    更新日期:2017-10-01 00:00:00

  • An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1.

    abstract::A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Okuda A,Kimura G

    更新日期:1996-03-15 00:00:00

  • EYA1 promotes tumor angiogenesis by activating the PI3K pathway in colorectal cancer.

    abstract::Blood vessels are one of the major routes for the dissemination of cancer cells. Malignant tumors release growth factors such as vascular endothelial growth factor(VEGF) to induce angiogenesis, thereby promoting metastasis. Here, we report that The Drosophila Eyes Absent Homologue 1 (EYA1), which is overexpressed in c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cai S,Cheng X,Liu Y,Lin Z,Zeng W,Yang C,Liu L,Chukwuebuka OA,Li W

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  • Heparin II domain of fibronectin mediates contractility through an alpha4beta1 co-signaling pathway.

    abstract::In the trabecular meshwork (TM) of the eye, regulation of tissue contractility by the PPRARI sequence within the Heparin II (HepII) domain of fibronectin is believed to control the movement of aqueous humor and dictate the level of intraocular pressure. This study shows that the HepII domain utilizes activated alpha4b...

    journal_title:Experimental cell research

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    authors: Schwinn MK,Gonzalez JM Jr,Gabelt BT,Sheibani N,Kaufman PL,Peters DM

    更新日期:2010-05-15 00:00:00

  • Coordination of growth and differentiation in the fetal lung.

    abstract::The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nielsen HC,Kirk WO,Sweezey N,Torday JS

    更新日期:1990-05-01 00:00:00

  • Coronin 3 involvement in F-actin-dependent processes at the cell cortex.

    abstract::The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rosentreter A,Hofmann A,Xavier CP,Stumpf M,Noegel AA,Clemen CS

    更新日期:2007-03-10 00:00:00

  • CUB domain-containing protein 1 (CDCP1) binds transforming growth factor beta family members and increase TGF-β1 signaling pathway.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Predes D,Cruz JVR,Abreu JG,Mendes FA

    更新日期:2019-10-01 00:00:00

  • The role of peroxisome proliferator-activated receptor-beta/delta in epidermal growth factor-induced HaCaT cell proliferation.

    abstract::Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

    更新日期:2008-10-15 00:00:00

  • Cultured endothelial cells produce multiple growth factors for connective tissue cells.

    abstract::Cultured bovine aortic endothelial cells (BAEC) secrete into their medium a growth-promoting factor that stimulates many connective tissue cells in culture. We now report that this growth-promoting activity is due to at least two different proteins which are biochemically separable and immunologically distinct. Cation...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: DiCorleto PE

    更新日期:1984-07-01 00:00:00

  • Trichostatin A affects the secretion pathways of beta and intestinal endocrine cells.

    abstract::Histone deacetylase inhibitors (HDACi) were recently identified as having significant clinical potential in reversing β-cell functional inhibition caused by inflammation, a shared precursor of Type 1 and Type 2 diabetes. However, HDACi are highly complex and little is known of their direct effect on important cell sec...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tiernan AR,Champion JA,Sambanis A

    更新日期:2015-01-01 00:00:00

  • Elimination of mycoplasmas from cell cultures utilizing hyperimmune sera.

    abstract::Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jeansson S,Brorson JE

    更新日期:1985-11-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract::Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhang J,Harrison JS,Studzinski GP

    更新日期:2011-01-01 00:00:00

  • Reactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST.

    abstract::An inactive human X chromosome was introduced by microcell fusion into two mouse embryonal carcinoma cell lines, PSA1-TG8 and OTF9-63, each of which has a single X chromosome. The donor cell line was a mouse-human somatic cell hybrid, CF150, retaining one or more inactive human X chromosome(s) per cell as its only hum...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yoshida I,Nishita Y,Mohandas TK,Takagi N

    更新日期:1997-02-01 00:00:00

  • Multiple proteases are involved in thymocyte apoptosis.

    abstract::To investigate the involvement of proteases in apoptosis, rat thymocytes were treated with the glucocorticoid hormone methylprednisolone or the topoisomerase II inhibitor etoposide in the presence of selective substrate inhibitors of either interleukin-1 beta-converting enzyme (ICE), (Z-Val-Ala-Asp-chloromethylketone,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhivotovsky B,Gahm A,Ankarcrona M,Nicotera P,Orrenius S

    更新日期:1995-12-01 00:00:00