Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.


:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the engulfment of apoptotic cells, the molecular bases by which an apoptotic cell is recognized are not entirely understood. Among the several potential mechanisms by which a macrophage can identify a cell as apoptotic, the data reported in the present paper support the idea that Kupffer cells phagocytose apoptotic cells by means of lectin-like receptors. Human Kupffer cells, which possess galactose-specific binding sites, can recognize and phagocytose peripheral blood lymphocytes undergoing apoptosis after heat shock (43 degrees C) or cycloheximide treatment, but not normal living peripheral blood lymphocytes. The putative structure by which apoptotic peripheral blood lymphocytes are targeted as "edible" could be the molecular changes in the plasma membrane, In fact, our experiments indicate that the membranes of apoptotic peripheral blood lymphocytes express increased amounts of N-acetylgalactosamine, D-galactose, and mannose residues when compared with membranes of normal PBL. Phagocytosis was inhibited by adding to the culture medium sugar cocktail solution (glucose, N-acetylgalactosamine, methyl mannopyranoside, fucose, 80 mM final concentration) or to a lower extent by desialylated glycoproteins (lactosylated bovine serum albumin, asialofetuin, 2 mg/ml final concentration), but not by nondesialylated glycoproteins (fetuin, 2 mg/ml final concentration, bovine serum albumin, 20% final concentration). In addition, phagocytosis of apoptotic peripheral blood lymphocytes by human Kupffer cells was a very rapid process, being almost entirely completed within 15 min of incubation.


Exp Cell Res


Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L




Has Abstract


1996-04-10 00:00:00














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    abstract::Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liu E,Minor RR,Horovitz O,Wootton JA,Podleski TR,Salpeter MM

    更新日期:1993-11-01 00:00:00

  • Conditional inactivation of Brca1 in the mouse ovarian surface epithelium results in an increase in preneoplastic changes.

    abstract::Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

    journal_title:Experimental cell research

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    authors: Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

    更新日期:2007-01-01 00:00:00

  • Induction of apoptosis by cancer chemotherapy.

    abstract::Studies performed over the past five years have demonstrated that there are two major cell-intrinsic pathways for inducing apoptosis, one that begins with ligation of cell surface death receptors and another that involves mitochondrial release of cytochrome c. Several reports have suggested that anticancer drugs kill ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Kaufmann SH,Earnshaw WC

    更新日期:2000-04-10 00:00:00

  • ER stress triggers apoptosis induced by Nogo-B/ASY overexpression.

    abstract::Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kuang E,Wan Q,Li X,Xu H,Zou T,Qi Y

    更新日期:2006-07-01 00:00:00

  • Decreased tumorigenicity of c-Myc-transformed fibroblasts expressing active USF2.

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    journal_title:Experimental cell research

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    authors: Choe C,Chen N,Sawadogo M

    更新日期:2005-01-01 00:00:00

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    abstract::There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...

    journal_title:Experimental cell research

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    authors: McElroy GS,Chandel NS

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  • Functional and phenotypic characterization of two HL60 clones resistant to dimethylsulfoxide.

    abstract::Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 months. The morphological and cytochemical features of the two populations are quite similar to those of the orig...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ferrero D,Gallo E,Lanfrancone L,Tarella C

    更新日期:1983-08-01 00:00:00

  • Induction of neurite outgrowth in PC12 cells by gamma-lactam-related compounds via Ras-MAP kinase signaling pathway independent mechanism.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yao R,Osada H

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pine PS,Weaver JL,Oravecz T,Pall M,Ussery M,Aszalos A

    更新日期:1998-04-10 00:00:00

  • RelA/NF-kappaB transcription factor associates with alpha-actinin-4.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Babakov VN,Petukhova OA,Turoverova LV,Kropacheva IV,Tentler DG,Bolshakova AV,Podolskaya EP,Magnusson KE,Pinaev GP

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    authors: Inohara H,Akahani S,Raz A

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    authors: Krishnamoorthy S,Jin R,Cai Y,Maddipati KR,Nie D,Pagès G,Tucker SC,Honn KV

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    authors: Danowski BA,Harris AK

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    authors: Rebelo TM,Vania L,Ferreira E,Weiss SFT

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  • Erythropoietin-dependent autocrine secretion of tumor necrosis factor-alpha in hematopoietic cells modulates proliferation via MAP kinase--ERK-1/2 and does not require tyrosine docking sites in the EPO receptor.

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    authors: Chen J,Jacobs-Helber SM,Barber DL,Sawyer ST

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  • Regulation of pyruvate kinase expression and growth in mastocytoma cells. I. Initial observations.

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    authors: Ngo JL,Chute H,Sanders DA,Orlando RA,Ibsen KH

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  • The timing of initiation of macronuclear DNA synthesis is set during the preceding cell cycle in Paramecium tetraurelia. Analysis of the effects of abrupt changes in nutrient level.

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    journal_title:Experimental cell research

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    authors: Gangopadhyay S,Jalali F,Reda D,Peacock J,Bristow RG,Benchimol S

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    authors: Fani N,Ziadlou R,Shahhoseini M,Baghaban Eslaminejad M

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Désiré L,Courtois Y,Jeanny JC

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tobey SL,McClelland KJ,Culp LA

    更新日期:1985-06-01 00:00:00

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Guo Z,Heydari A,Richardson A

    更新日期:1998-11-25 00:00:00