Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.


:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the engulfment of apoptotic cells, the molecular bases by which an apoptotic cell is recognized are not entirely understood. Among the several potential mechanisms by which a macrophage can identify a cell as apoptotic, the data reported in the present paper support the idea that Kupffer cells phagocytose apoptotic cells by means of lectin-like receptors. Human Kupffer cells, which possess galactose-specific binding sites, can recognize and phagocytose peripheral blood lymphocytes undergoing apoptosis after heat shock (43 degrees C) or cycloheximide treatment, but not normal living peripheral blood lymphocytes. The putative structure by which apoptotic peripheral blood lymphocytes are targeted as "edible" could be the molecular changes in the plasma membrane, In fact, our experiments indicate that the membranes of apoptotic peripheral blood lymphocytes express increased amounts of N-acetylgalactosamine, D-galactose, and mannose residues when compared with membranes of normal PBL. Phagocytosis was inhibited by adding to the culture medium sugar cocktail solution (glucose, N-acetylgalactosamine, methyl mannopyranoside, fucose, 80 mM final concentration) or to a lower extent by desialylated glycoproteins (lactosylated bovine serum albumin, asialofetuin, 2 mg/ml final concentration), but not by nondesialylated glycoproteins (fetuin, 2 mg/ml final concentration, bovine serum albumin, 20% final concentration). In addition, phagocytosis of apoptotic peripheral blood lymphocytes by human Kupffer cells was a very rapid process, being almost entirely completed within 15 min of incubation.


Exp Cell Res


Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L




Has Abstract


1996-04-10 00:00:00














  • Mevalonate is essential for growth activation of human fibroblasts: evidence for a critical role of protein glycosylation in the prereplicative period.

    abstract::Human diploid fibroblasts, arrested following serum or mevalonate depletion, were restimulated to a maximal rate of DNA synthesis within 24 h after the addition of serum or mevalonate, respectively. In both cases the initiation of DNA synthesis was preceded by a 12-h prereplicative phase. Upon the stimulation with ser...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Carlberg M,Hjertman M,Wejde J,Larsson O

    更新日期:1994-06-01 00:00:00

  • R5, a neutralizing antibody to Robo1, suppresses breast cancer growth and metastasis by inhibiting angiogenesis via down-regulating filamin A.

    abstract::Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

    更新日期:2020-02-01 00:00:00

  • Hydrocortisone-induced increase of PDGF beta-receptor expression in a human malignant mesothelioma cell line.

    abstract::The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-recepto...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Versnel MA,Bouts MJ,Langerak AW,van der Kwast TH,Hoogsteden HC,Hagemeijer A,Heldin CH

    更新日期:1992-05-01 00:00:00

  • Cleavage of Bax enhances its cell death function.

    abstract::Members of the Bcl-2 family of proteins are key regulators of apoptosis. Some of these proteins undergo posttranslational modification, such as phosphorylation or proteolysis, that serves to alter their function. Caspases are known to cleave Bid, a proapoptotic family member, as well as Bcl-2 and Bcl-X(L), two prosurv...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wood DE,Newcomb EW

    更新日期:2000-05-01 00:00:00

  • Simultaneous cell death and desquamation of the embryonic diffusion barrier during epidermal development.

    abstract::The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Saathoff M,Blum B,Quast T,Kirfel G,Herzog V

    更新日期:2004-10-01 00:00:00

  • AP4A-hydrolysing activity in sea urchin embryos.

    abstract::The enzyme activity hydrolysing diadenosine 5,5'-P1, P4-tetraphosphate (AP4A) was demonstrated in the embryonic extract of sea urchin. The enzyme activity was preferentially inhibited by ZnCl2 and by high concentrations of isobutylmethylxanthine, indicating that two types of the enzyme, (AP4A) hydrolase and non-specif...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Morioka M,Shimada H

    更新日期:1987-03-01 00:00:00

  • Cultured astrocytes form a syncytium after maturation.

    abstract::The formation of functional gap junctions between astrocytes was investigated during differentiation of these cells in culture. Precursor cells of GFA (glial fibrillary acidic) protein-positive astrocytes were cultured in a chemically defined medium as a homogeneous population. These cells were rarely coupled to one n...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fischer G,Kettenmann H

    更新日期:1985-08-01 00:00:00

  • High expression levels of the "erythroid/brain" type glucose transporter (GLUT1) in the basal cells of human eye conjunctiva and oral mucosa reconstituted in culture.

    abstract::The expression of the "erythroid/brain" type glucose transporter (GLUT1) seems to be a feature of "barrier" tissues, at least in humans. Recently, we reported that GLUT1 is highly expressed in the basal layers of either "authentic" human epidermis or human epidermis reconstituted in culture and that its expression see...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gherzi R,Melioli G,De Luca M,D'Agostino A,Guastella M,Traverso CE,D'Anna F,Franzi AT,Cancedda R

    更新日期:1991-07-01 00:00:00

  • A melanoma cell line sensitive to expression of a fusion protein binding the retinoblastoma protein family pocket domain.

    abstract::An established melanoma cell line (MM96L) was transfected with selectable plasmid constructs encoding either whole SV40 large T antigen, or beta-galactosidase fusions with the retinoblastoma protein (Rb)-binding region of SV40 large T antigen and a nonbinding mutant derivative of it. Both of the beta-galactosidase fus...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Khan S,Collins RJ,Dunn IS

    更新日期:1996-02-25 00:00:00

  • Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes.

    abstract::The calcium/calmodulin-dependent protein phosphatase calcineurin is required for the induction of transcriptional events that initiate and promote myogenic differentiation. An important effector for calcineurin in striated muscle is the transcription factor myocyte enhancer factor 2 (MEF2). The targeting of the enzyme...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Li J,Vargas MA,Kapiloff MS,Dodge-Kafka KL

    更新日期:2013-02-15 00:00:00

  • Hypomethylation of human heterochromatin detected by restriction enzyme nick translation.

    abstract::Using the restriction enzymes MspI and HpaII in the nick translation procedure it has been shown that decondensation of the paracentric heterochromatin of chromosome 9 during human spermatogenesis is associated with hypomethylation of the DNA sequences in this domain. Somatic cells treated with 5'-azacytidine also sho...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mitchell AR

    更新日期:1992-09-01 00:00:00

  • Recent progress in protocadherin research.

    abstract::Protocadherins constitute a large family belonging to the cadherin superfamily and function in different tissues of a wide variety of multicellular organisms. Protocadherins have unique features that are not found in classic cadherins. Expression of protocadherins is spatiotemporally regulated and they are localized a...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Suzuki ST

    更新日期:2000-11-25 00:00:00

  • Corneodesmosin, a corneodesmosome-specific basic protein, is expressed in the cornified epithelia of the pig, guinea pig, rat, and mouse.

    abstract::Proteolysis of corneodesmosin, a 52- to 56-kDa basic protein located in the extracellular part of the modified desmosomes (corneodesmosomes) of human cornified epithelia, is thought to be a key event of desquamation. Three monoclonal antibodies specific for human corneodesmosin were used to search for the expression o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Montézin M,Simon M,Guerrin M,Serre G

    更新日期:1997-02-25 00:00:00

  • Sendai virus and herpes virus type 1 induce apoptosis in human peripheral blood mononuclear cells.

    abstract::Recent reports suggest that several viruses, besides human immunodeficiency virus, induce apoptosis in infected cells. We report here that Sendai virus or Herpes simplex virus type 1 (HSV-1), two potent inducers of interferon-alpha, caused cell death in a consistent number of human peripheral blood mononuclear cells. ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Tropea F,Troiano L,Monti D,Lovato E,Malorni W,Rainaldi G,Mattana P,Viscomi G,Ingletti MC,Portolani M

    更新日期:1995-05-01 00:00:00

  • Telomere reduction in human liver tissues with age and chronic inflammation.

    abstract::Telomere shortening in human liver with aging and chronic inflammation was examined by hybridization protection assay using telomere and Alu probes. The reduction rate of telomere repeats in normal liver (23 samples from patients 17-81 years old) was 120 bp per year, which is in good agreement with the reported reduct...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Aikata H,Takaishi H,Kawakami Y,Takahashi S,Kitamoto M,Nakanishi T,Nakamura Y,Shimamoto F,Kajiyama G,Ide T

    更新日期:2000-05-01 00:00:00

  • Glucocorticoid hormones may partially substitute for adenovirus E1A in cooperation with ras.

    abstract::The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

    更新日期:1991-05-01 00:00:00

  • Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase.

    abstract::Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ek-Rylander B,Andersson G

    更新日期:2010-02-01 00:00:00

  • Internalization of the hyaluronan receptor CD44 by chondrocytes.

    abstract::Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Aguiar DJ,Knudson W,Knudson CB

    更新日期:1999-11-01 00:00:00

  • Negative cooperativity between alpha 3 beta 1 and alpha 2 beta 1 integrins in human mammary carcinoma MDA MB 231 cells.

    abstract::The alpha 3 beta 1 integrin has been implicated as a receptor for several matrix components, including collagen, fibronectin, and laminins. The function of alpha 3 beta 1 seems to be very versatile involving cell adhesion to or migration on ECM, establishment of cell-cell contacts in aggregates, as well as linkage to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lichtner RB,Howlett AR,Lerch M,Xuan JA,Brink J,Langton-Webster B,Schneider MR

    更新日期:1998-05-01 00:00:00

  • Down-regulation of UDP-glucose dehydrogenase affects glycosaminoglycans synthesis and motility in HCT-8 colorectal carcinoma cells.

    abstract::UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wang TP,Pan YR,Fu CY,Chang HY

    更新日期:2010-10-15 00:00:00

  • Chondrocyte and chondrosarcoma cell integrins with affinity for collagen type II and their response to mechanical stress.

    abstract::Mechanical stress is an important regulator of chondrocyte functions but the mechanisms by which chondrocytes sense mechanical signals are unknown. Receptors for matrix molecules are likely involved in the mechanical signaling. In the first part of this study we identified integrins with affinity for the cartilage-spe...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Holmvall K,Camper L,Johansson S,Kimura JH,Lundgren-Akerlund E

    更新日期:1995-12-01 00:00:00

  • Resistance to DNA denaturation in irradiated Chinese hamster V79 fibroblasts is linked to cell shape.

    abstract::Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Olive PL,Vanderbyl S,MacPhail SH

    更新日期:1991-04-01 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract::NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Durkin JP,Whitfield JF

    更新日期:1985-04-01 00:00:00

  • Bacterial heat shock protein-60 increases epithelial cell proliferation through the ERK1/2 MAP kinases.

    abstract::Heat shock proteins (hsp) have important roles in the regulation and protection of both prokaryotic and eukaryotic cells, especially during environmental stress. Hsps are also important bacterial virulence factors. We investigated whether bacterial hsp60 can alter epithelial cell mitogen-activated protein kinase (MAPK...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhang L,Pelech SL,Mayrand D,Grenier D,Heino J,Uitto VJ

    更新日期:2001-05-15 00:00:00

  • A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes.

    abstract::It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

    更新日期:2006-08-01 00:00:00

  • Establishment of medakafish as a model for stem cell-based gene therapy: efficient gene delivery and potential chromosomal integration by baculoviral vectors.

    abstract::Viral vectors hold promise and challenges in gene therapy. Specifically, we have previously shown that baculoviral (BV) vectors have a high efficiency of gene delivery in human embryonic stem (ES) cells. Here we report the development of a complementary system to further our evaluation by utilizing the laboratory fish...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yan Y,Du J,Chen T,Yi M,Li M,Wang S,Li CM,Hong Y

    更新日期:2009-08-01 00:00:00

  • Long-term culture and differentiation of CNS precursors derived from anterior human neural rosettes following exposure to ventralizing factors.

    abstract::In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proli...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Colleoni S,Galli C,Giannelli SG,Armentero MT,Blandini F,Broccoli V,Lazzari G

    更新日期:2010-04-15 00:00:00

  • MZF-1 and DbpA interact with DNase I hypersensitive sites that correlate with expression of the human MUC1 mucin gene.

    abstract::The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

    更新日期:2005-08-01 00:00:00

  • Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes.

    abstract::TLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-α, IL-6, IL-10 and MCP-1 expres...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grabulosa CC,Manfredi SR,Canziani ME,Quinto BMR,Barbosa RB,Rebello JF,Batista MC,Cendoroglo M,Dalboni MA

    更新日期:2018-04-15 00:00:00

  • Overexpression of glial cell line-derived neurotrophic factor induces genes regulating migration and differentiation of neuronal progenitor cells.

    abstract::The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

    更新日期:2004-07-15 00:00:00