Abstract:
:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the engulfment of apoptotic cells, the molecular bases by which an apoptotic cell is recognized are not entirely understood. Among the several potential mechanisms by which a macrophage can identify a cell as apoptotic, the data reported in the present paper support the idea that Kupffer cells phagocytose apoptotic cells by means of lectin-like receptors. Human Kupffer cells, which possess galactose-specific binding sites, can recognize and phagocytose peripheral blood lymphocytes undergoing apoptosis after heat shock (43 degrees C) or cycloheximide treatment, but not normal living peripheral blood lymphocytes. The putative structure by which apoptotic peripheral blood lymphocytes are targeted as "edible" could be the molecular changes in the plasma membrane, In fact, our experiments indicate that the membranes of apoptotic peripheral blood lymphocytes express increased amounts of N-acetylgalactosamine, D-galactose, and mannose residues when compared with membranes of normal PBL. Phagocytosis was inhibited by adding to the culture medium sugar cocktail solution (glucose, N-acetylgalactosamine, methyl mannopyranoside, fucose, 80 mM final concentration) or to a lower extent by desialylated glycoproteins (lactosylated bovine serum albumin, asialofetuin, 2 mg/ml final concentration), but not by nondesialylated glycoproteins (fetuin, 2 mg/ml final concentration, bovine serum albumin, 20% final concentration). In addition, phagocytosis of apoptotic peripheral blood lymphocytes by human Kupffer cells was a very rapid process, being almost entirely completed within 15 min of incubation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Falasca L,Bergamini A,Serafino A,Balabaud C,Dini Ldoi
10.1006/excr.1996.0123subject
Has Abstractpub_date
1996-04-10 00:00:00pages
152-62issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(96)90123-8journal_volume
224pub_type
杂志文章abstract:BACKGROUND:Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absen...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.09.015
更新日期:2012-01-15 00:00:00
abstract::A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxicall...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90204-2
更新日期:1988-04-01 00:00:00
abstract::The long non-coding RNAs (lncRNAs) have gained much attention due to its essential roles in molecular regulation. As one of the classic lncRNAs, H19 is strongly expressed during embryogenesis and plays a crucial biological function during development. Mesenchymal stem cells (MSCs) are an ideal cell source for tissue e...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2017.09.007
更新日期:2017-11-15 00:00:00
abstract::The bone-related transcription factor Runx2 (Cbfa1) has been extensively shown to regulate osteoblast differentiation and function. Recent studies demonstrate that Runx2 is also a positive regulator of chondrocyte maturation and vascular invasion in cartilage. Runx2 activity can be modulated in several ways, including...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.05.025
更新日期:2004-09-10 00:00:00
abstract::In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90117-0
更新日期:1987-05-01 00:00:00
abstract::Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT,...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(02)00055-1
更新日期:2003-02-15 00:00:00
abstract::Mammalian enabled (MENA), usually known as a direct regulator of microfilament polymerization and bundling, promotes metastasis in various cancers. Here we focus on the role of MENA in hepatocellular carcinoma (HCC) metastasis and the relevant mechanism from the view of RhoA activity regulation. By HCC tissue microarr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.05.010
更新日期:2014-09-10 00:00:00
abstract::Rat glomerular epithelial cells were grown to confluency on semipermeable tissue culture inserts and the plasminogen system of these cells was analyzed using enzyme assays, Western analysis, zymography, and reverse transcriptase-PCR. The glomerular epithelial cells are capable of activating exogenous plasminogen to pl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1191
更新日期:1995-06-01 00:00:00
abstract::Transferrin (Tf), an iron-binding protein, was investigated in the cultured human fibroblast which is a major cell type in connective tissues. Tf is a major iron-transporting protein and has an important role in iron metabolism. Using two-dimensional gel electrophoresis, we demonstrated the eight subtypes of Tf produc...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4080
更新日期:1998-07-10 00:00:00
abstract::In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3631
更新日期:1997-08-01 00:00:00
abstract::Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.02.032
更新日期:2004-07-15 00:00:00
abstract::Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subse...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.03.009
更新日期:2009-07-01 00:00:00
abstract::Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.03.002
更新日期:2013-05-01 00:00:00
abstract::Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4681
更新日期:1999-12-15 00:00:00
abstract::Abdominal aortic aneurysms (AAA) are characterized by matrix remodeling, elastin degradation, absence of nitric oxide (NO) signaling, and inflammation, influencing smooth muscle cell (SMC) phenotype and gene expression. Little is known about the biomolecular release and intrinsic biomechanics of human AAA-SMCs. NO del...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.111589
更新日期:2019-11-01 00:00:00
abstract::Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.12.027
更新日期:2013-03-10 00:00:00
abstract::Considering that stimulation of melanogenesis may lead to alterations of cellular responses, besides melanin production, our main goal was to study the cellular effects of melanogenesis stimulation of B16-F10 melanoma cells. Our results show increased levels of the reactive oxygen species after 15 h of melanogenesis s...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.05.019
更新日期:2012-09-10 00:00:00
abstract::The progression of cancer cells to highly malignant phenotypes that ultimately invade and metastasize to near and distant sites requires that metastatic cells respond to mitogenic signals in new microenvironments. The successful growth of metastases is due to their ability to respond to local paracrine growth factors ...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1006/excr.1993.1022
更新日期:1993-02-01 00:00:00
abstract::Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.02.020
更新日期:2010-07-15 00:00:00
abstract::Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2009.07.023
更新日期:2009-11-15 00:00:00
abstract::Among different cancers, incidence and mortality of colorectal cancer (CRC) is one of the highest. KRAS mutation is one of the underlying features in the pathogenesis of CRC with CRC tumors harboring mutant KRAS exhibiting a more aggressive behavior compared to CRC tumors with wild type KRAS. We had earlier shown that...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2018.08.016
更新日期:2018-12-15 00:00:00
abstract::Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfl...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(87)90176-5
更新日期:1987-08-01 00:00:00
abstract::The macrophages have different subtypes with different functions in immune response and disease. It has been generally accepted that M1 macrophages are responsible for stimulation of immune system and inflammation while M2 macrophages play a role in tissue repair. Irrespective of the type, macrophage functions depend ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2016.02.004
更新日期:2016-02-15 00:00:00
abstract::Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90431-8
更新日期:1985-05-01 00:00:00
abstract::Prior studies have shown that synthetic peptides representing the domain of thrombin responsible for high-affinity binding to fibroblasts stimulate chemotactic and cell proliferative signals through a nonproteolytic mechanism. One of these peptides, TP508, has recently been shown to be chemotactic for neutrophils, to ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1998.4372
更新日期:1999-03-15 00:00:00
abstract::It is known that DNA fragmentation during apoptosis is controlled by a number of factors, a crucial step being the caspase-operated cleavage of ICAD, the DNase inhibitor. We have previously demonstrated that hydrogen peroxide-treated lymphocytes undergo apoptosis without formation of a DNA ladder; however, the use of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5222
更新日期:2001-06-10 00:00:00
abstract::Alexander disease (AxD) is a rare neurodegenerative disorder characterized by large cytoplasmic aggregates in astrocytes and myelin abnormalities and caused by dominant mutations in the gene encoding glial fibrillary acidic protein (GFAP), the main intermediate filament protein in astrocytes. We tested the effects of ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.04.035
更新日期:2007-08-01 00:00:00
abstract::We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2007.01.003
更新日期:2007-04-15 00:00:00
abstract::NIH3T3 cells carrying a dominant negative H-ras mutant 116Y acquired resistance to transformation by some PTK oncogenes, i.e., v-fes, v-abl, and v-fms, but were sensitive to viral ras and serine threonine kinase oncogenes, v-raf and v-mos. One clone, designated 1-20, infected with v-fes (1-20 fes) exhibited flat morph...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1263
更新日期:1993-10-01 00:00:00
abstract::Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4641
更新日期:1999-11-01 00:00:00